The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1001 http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 https://doi.org/10.1016/j.vaccine.2016.04.056 |
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| author | Rabinovitz, Bettina Carol Larzabal, Mariano Vilte, Daniel Alejandro Cataldi, Angel Adrian Mercado, Elsa Cristina |
| author_browse | Cataldi, Angel Adrian Larzabal, Mariano Mercado, Elsa Cristina Rabinovitz, Bettina Carol Vilte, Daniel Alejandro |
| author_facet | Rabinovitz, Bettina Carol Larzabal, Mariano Vilte, Daniel Alejandro Cataldi, Angel Adrian Mercado, Elsa Cristina |
| author_sort | Rabinovitz, Bettina Carol |
| collection | INTA Digital |
| description | Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. |
| format | Artículo |
| id | INTA1001 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10012018-02-23T15:44:22Z The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection Rabinovitz, Bettina Carol Larzabal, Mariano Vilte, Daniel Alejandro Cataldi, Angel Adrian Mercado, Elsa Cristina Escherichia coli Vaccination Mice Infection Vacunación Ratón Infección Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2017-08-22T13:22:37Z 2017-08-22T13:22:37Z 2016-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1001 http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 0264-410X https://doi.org/10.1016/j.vaccine.2016.04.056 eng info:eu-repo/semantics/restrictedAccess application/pdf Vaccine 34 (25) : 2793-2797. (May 2016) |
| spellingShingle | Escherichia coli Vaccination Mice Infection Vacunación Ratón Infección Rabinovitz, Bettina Carol Larzabal, Mariano Vilte, Daniel Alejandro Cataldi, Angel Adrian Mercado, Elsa Cristina The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
| title | The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
| title_full | The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
| title_fullStr | The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
| title_full_unstemmed | The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
| title_short | The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
| title_sort | intranasal vaccination of pregnant dams with intimin and espbconfers protection in neonatal mice from escherichia coli ehec o157 h7 infection |
| topic | Escherichia coli Vaccination Mice Infection Vacunación Ratón Infección |
| url | http://hdl.handle.net/20.500.12123/1001 http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 https://doi.org/10.1016/j.vaccine.2016.04.056 |
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