Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because o...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Longdom Publishing
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10000 https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+ https://doi.org/10.35248/2165-8056.19.9.160 |
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| author | Arias, Renee S. Cazon, Luis Ignacio Massa, Alicia N. Scheffler, Brian E. Sobolev, Victor S. Lamb, Marshall C. Duke, Mary V. Simpson, Sheron A. Conforto, Erica Cinthia Paredes, Juan Andrés Soave, Juan H. Buteler, Mario I. Rago, Alejandro Mario |
| author_browse | Arias, Renee S. Buteler, Mario I. Cazon, Luis Ignacio Conforto, Erica Cinthia Duke, Mary V. Lamb, Marshall C. Massa, Alicia N. Paredes, Juan Andrés Rago, Alejandro Mario Scheffler, Brian E. Simpson, Sheron A. Soave, Juan H. Sobolev, Victor S. |
| author_facet | Arias, Renee S. Cazon, Luis Ignacio Massa, Alicia N. Scheffler, Brian E. Sobolev, Victor S. Lamb, Marshall C. Duke, Mary V. Simpson, Sheron A. Conforto, Erica Cinthia Paredes, Juan Andrés Soave, Juan H. Buteler, Mario I. Rago, Alejandro Mario |
| author_sort | Arias, Renee S. |
| collection | INTA Digital |
| description | Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to
provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes.
Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides.
Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10000 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Longdom Publishing |
| publisherStr | Longdom Publishing |
| record_format | dspace |
| spelling | INTA100002021-08-11T11:25:53Z Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut Arias, Renee S. Cazon, Luis Ignacio Massa, Alicia N. Scheffler, Brian E. Sobolev, Victor S. Lamb, Marshall C. Duke, Mary V. Simpson, Sheron A. Conforto, Erica Cinthia Paredes, Juan Andrés Soave, Juan H. Buteler, Mario I. Rago, Alejandro Mario Pathogens Groundnuts Fungicides Fungicidas Organismos Patógenos Cacahuete Arachis hypogaea Mitochondrial Genome Carbón del Mani Thecaphora Frezii Maní Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci. Instituto de Patología Vegetal Fil: Arias, Renee S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Massa, Alicia N. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Scheffler, Brian E. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil: Sobolev, Victor S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Lamb, Marshall C. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Duke, Mary V. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil: Simpson, Sheron A. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Soave, Juan H. El Carmen S.A. General Cabrera; Argentina Fil: Buteler, Mario I. El Carmen S.A. General Cabrera; Argentina Fil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2021-08-11T11:08:42Z 2021-08-11T11:08:42Z 2019-08-28 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10000 https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+ 2165-8056 https://doi.org/10.35248/2165-8056.19.9.160 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Longdom Publishing Fungal Genomics & Biology 9 (160) : 1-8 (2019) |
| spellingShingle | Pathogens Groundnuts Fungicides Fungicidas Organismos Patógenos Cacahuete Arachis hypogaea Mitochondrial Genome Carbón del Mani Thecaphora Frezii Maní Arias, Renee S. Cazon, Luis Ignacio Massa, Alicia N. Scheffler, Brian E. Sobolev, Victor S. Lamb, Marshall C. Duke, Mary V. Simpson, Sheron A. Conforto, Erica Cinthia Paredes, Juan Andrés Soave, Juan H. Buteler, Mario I. Rago, Alejandro Mario Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
| title | Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
| title_full | Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
| title_fullStr | Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
| title_full_unstemmed | Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
| title_short | Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
| title_sort | mitogenome and nuclear encoded fungicide target genes of thecaphora frezii causal agent of peanut smut |
| topic | Pathogens Groundnuts Fungicides Fungicidas Organismos Patógenos Cacahuete Arachis hypogaea Mitochondrial Genome Carbón del Mani Thecaphora Frezii Maní |
| url | http://hdl.handle.net/20.500.12123/10000 https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+ https://doi.org/10.35248/2165-8056.19.9.160 |
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