Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru
Potato (Solanum tuberosum L.) is a key staple crop in the Peruvian Andes, but its productivity is threatened by fungal pathogens such as Rhizoctonia solani and Alternaria alternata. In this study, 71 native bacterial strains (39 from phyllosphere and 32 from rhizosphere) were isolated from potato pl...
| Autores principales: | , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
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MDPI
2026
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12955/2987 https://doi.org/10.3390/applmicrobiol6010002 |
| _version_ | 1855028794413285376 |
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| author | Mamani Rojas, Lizbeth Margarita Rengifo Sánchez, Raihil Rabindranath Velarde Apaza, Leslie Diana Ramírez Rojas, Max Cántaro Segura, Hector |
| author_browse | Cántaro Segura, Hector Mamani Rojas, Lizbeth Margarita Ramírez Rojas, Max Rengifo Sánchez, Raihil Rabindranath Velarde Apaza, Leslie Diana |
| author_facet | Mamani Rojas, Lizbeth Margarita Rengifo Sánchez, Raihil Rabindranath Velarde Apaza, Leslie Diana Ramírez Rojas, Max Cántaro Segura, Hector |
| author_sort | Mamani Rojas, Lizbeth Margarita |
| collection | Repositorio INIA |
| description | Potato (Solanum tuberosum L.) is a key staple crop in the Peruvian Andes, but its productivity is threatened by fungal pathogens such as Rhizoctonia solani and Alternaria alternata. In this study, 71 native bacterial strains (39 from phyllosphere and 32 from rhizosphere) were isolated from potato plants across five agroecological zones of Peru and characterized for their plant growth-promoting (PGPR) and antagonistic traits. Actinomycetes demonstrated broader enzymatic profiles, with 2ACPP4 and 2ACPP8 showing high proteolytic (68.4%, 63.4%), lipolytic (59.5%, 60.6%), chitinolytic (32.7%, 35.5%) and amylolytic activity (76.3%, 71.5%). Strain 5ACPP5 (Streptomyces decoyicus) produced 42.8% chitinase and solubilized both dicalcium (120.6%) and tricalcium phosphate (122.3%). The highest IAA production was recorded in Bacillus strain 2BPP8 (95.4 µg/mL), while 5ACPP6 was the highest among Actinomycetes (83.4 µg/mL). Siderophore production was highest in 5ACPP5 (412.4%) and 2ACPP4 (406.8%). In vitro antagonism assays showed that 5ACPP5 inhibited R. solani and A. alternata by 86.4% and 68.9%, respectively, while Bacillus strain BPP4 reached 51.0% inhibition against A. alternata. In greenhouse trials, strain 4BPP8 significantly increased fresh tuber weight (11.91 g), while 5ACPP5 enhanced root biomass and reduced stem canker severity. Molecular identification confirmed BPP4 as Bacillus halotolerans and 5ACPP5 as Streptomyces decoyicus. These strains represent promising candidates for the development of bioinoculants for sustainable potato cultivation in Andean systems. |
| format | info:eu-repo/semantics/article |
| id | INIA2987 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INIA29872026-01-15T21:43:13Z Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru Mamani Rojas, Lizbeth Margarita Rengifo Sánchez, Raihil Rabindranath Velarde Apaza, Leslie Diana Ramírez Rojas, Max Cántaro Segura, Hector Solanum tuberosum Bacillus Actinomycetes Plant growth-promoting rhizobacteria Rhizoctonia Alternaria Sustainable agriculture Microbiome Actinomicetos Rizobacterias promotoras del crecimiento vegetal Agricultura sostenible Microbioma https://purl.org/pe-repo/ocde/ford#4.04.01 Papa; Potatoes; Rizosfera; Rhizosphere; Control biológico; Biological control; Agricultura sostenible; Sustainable agriculture Potato (Solanum tuberosum L.) is a key staple crop in the Peruvian Andes, but its productivity is threatened by fungal pathogens such as Rhizoctonia solani and Alternaria alternata. In this study, 71 native bacterial strains (39 from phyllosphere and 32 from rhizosphere) were isolated from potato plants across five agroecological zones of Peru and characterized for their plant growth-promoting (PGPR) and antagonistic traits. Actinomycetes demonstrated broader enzymatic profiles, with 2ACPP4 and 2ACPP8 showing high proteolytic (68.4%, 63.4%), lipolytic (59.5%, 60.6%), chitinolytic (32.7%, 35.5%) and amylolytic activity (76.3%, 71.5%). Strain 5ACPP5 (Streptomyces decoyicus) produced 42.8% chitinase and solubilized both dicalcium (120.6%) and tricalcium phosphate (122.3%). The highest IAA production was recorded in Bacillus strain 2BPP8 (95.4 µg/mL), while 5ACPP6 was the highest among Actinomycetes (83.4 µg/mL). Siderophore production was highest in 5ACPP5 (412.4%) and 2ACPP4 (406.8%). In vitro antagonism assays showed that 5ACPP5 inhibited R. solani and A. alternata by 86.4% and 68.9%, respectively, while Bacillus strain BPP4 reached 51.0% inhibition against A. alternata. In greenhouse trials, strain 4BPP8 significantly increased fresh tuber weight (11.91 g), while 5ACPP5 enhanced root biomass and reduced stem canker severity. Molecular identification confirmed BPP4 as Bacillus halotolerans and 5ACPP5 as Streptomyces decoyicus. These strains represent promising candidates for the development of bioinoculants for sustainable potato cultivation in Andean systems. The National Institute of Agrarian Innovation (INIA), Peru, funded this research within the framework of the investment project “Improvement of agricultural research and technology transfer services at the El Chira—Marcavelica Agricultural Experimental Station in the Marcavelica District, Sullana Province, Piura Department”, with CUI No. 2472190. 2026-01-09T16:12:55Z 2026-01-09T16:12:55Z 2025-12-23 info:eu-repo/semantics/article Mamani-Rojas, L., Rengifo, R., Velarde-Apaza, L., Ramírez-Rojas, M., & Cántaro-Segura, H. (2025). Biocontrol and plant growth-promoting potential of Bacillus and Actinomycetes isolated from the rhizosphere and phyllosphere of potato (Solanum tuberosum L.) from different agroecological zones of Peru. Applied Microbiology, 6(1), 2. https://doi.org/10.3390/applmicrobiol6010002 2673-8007 http://hdl.handle.net/20.500.12955/2987 https://doi.org/10.3390/applmicrobiol6010002 eng urn:issn:2673-8007 Applied Microbiology info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf MDPI CH Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Solanum tuberosum Bacillus Actinomycetes Plant growth-promoting rhizobacteria Rhizoctonia Alternaria Sustainable agriculture Microbiome Actinomicetos Rizobacterias promotoras del crecimiento vegetal Agricultura sostenible Microbioma https://purl.org/pe-repo/ocde/ford#4.04.01 Papa; Potatoes; Rizosfera; Rhizosphere; Control biológico; Biological control; Agricultura sostenible; Sustainable agriculture Mamani Rojas, Lizbeth Margarita Rengifo Sánchez, Raihil Rabindranath Velarde Apaza, Leslie Diana Ramírez Rojas, Max Cántaro Segura, Hector Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru |
| title | Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru |
| title_full | Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru |
| title_fullStr | Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru |
| title_full_unstemmed | Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru |
| title_short | Biocontrol and Plant Growth-Promoting Potential of Bacillus and Actinomycetes Isolated from the Rhizosphere and Phyllosphere of Potato (Solanum tuberosum L.) from Different Agroecological Zones of Peru |
| title_sort | biocontrol and plant growth promoting potential of bacillus and actinomycetes isolated from the rhizosphere and phyllosphere of potato solanum tuberosum l from different agroecological zones of peru |
| topic | Solanum tuberosum Bacillus Actinomycetes Plant growth-promoting rhizobacteria Rhizoctonia Alternaria Sustainable agriculture Microbiome Actinomicetos Rizobacterias promotoras del crecimiento vegetal Agricultura sostenible Microbioma https://purl.org/pe-repo/ocde/ford#4.04.01 Papa; Potatoes; Rizosfera; Rhizosphere; Control biológico; Biological control; Agricultura sostenible; Sustainable agriculture |
| url | http://hdl.handle.net/20.500.12955/2987 https://doi.org/10.3390/applmicrobiol6010002 |
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