Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles

The lily stands out as one of the most economically relevant flowers worldwide and enjoys great popularity as a cut flower in the florist industry. This prominent position underlines the need to implement and continuously improve sustainable protocols that ensure its efficient production, multiplica...

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Main Authors: Tomanguilla Llanos, Oscar, Meléndez Mori, Jegnes Benjamín, Tejada Alvarado, José Jesús, Lapiz Culqui, Yoiner K., Huaman Huaman, Eyner, Zuta Puscan, Marileydi, Mego Pérez, Reyna Stefani, Oliva Cruz, Manuel
Format: Artículo
Language:Inglés
Published: Springer Nature 2025
Subjects:
Online Access:http://hdl.handle.net/20.500.12955/2817
https://doi.org/10.1038/s41598-025-06078-7
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author Tomanguilla Llanos, Oscar
Meléndez Mori, Jegnes Benjamín
Tejada Alvarado, José Jesús
Lapiz Culqui, Yoiner K.
Huaman Huaman, Eyner
Zuta Puscan, Marileydi
Mego Pérez, Reyna Stefani
Oliva Cruz, Manuel
author_browse Huaman Huaman, Eyner
Lapiz Culqui, Yoiner K.
Mego Pérez, Reyna Stefani
Meléndez Mori, Jegnes Benjamín
Oliva Cruz, Manuel
Tejada Alvarado, José Jesús
Tomanguilla Llanos, Oscar
Zuta Puscan, Marileydi
author_facet Tomanguilla Llanos, Oscar
Meléndez Mori, Jegnes Benjamín
Tejada Alvarado, José Jesús
Lapiz Culqui, Yoiner K.
Huaman Huaman, Eyner
Zuta Puscan, Marileydi
Mego Pérez, Reyna Stefani
Oliva Cruz, Manuel
author_sort Tomanguilla Llanos, Oscar
collection Repositorio INIA
description The lily stands out as one of the most economically relevant flowers worldwide and enjoys great popularity as a cut flower in the florist industry. This prominent position underlines the need to implement and continuously improve sustainable protocols that ensure its efficient production, multiplication and conservation. In this context, the present study aimed to investigate the potential biostimulant effect of Silver Nanoparticles (AgNPs) and 6-Benzylaminopurine (BAP) on two lily cultivars (Lilium LA Hybrid ‘Hyde Park’ and Lilium LA Hybrid ‘Yellow Diamond’), through their individual and combined application. The research focused on evaluating the influence of these treatments on two key aspects: (1) regeneration capacity and bulblet development from bulb scales, and (2) morpho-physiological growth parameters in regenerated plants. Analysis of the results revealed several significant effects of the interaction between AgNPs and BAP on different regeneration and growth parameters in two lily cultivars. In cv. ‘Hyde Park’, the combination of AgNPs at 50 mg L ⁻¹ with BAP at 200 mg L ⁻¹ increased bulblet number to an average of 5.6. In cv. ‘Yellow Diamond’, the application of AgNPs at 50 mg L ⁻¹ without BAP and the combined treatment of AgNPs at 100 mg L ⁻¹ with BAP at 200 mg L ⁻¹ produced a higher number of bulblets (3.2). The highest bulblet weight in both cultivars was obtained with the combination of AgNPs at 100 mg L ⁻¹ and BAP at 50 mg L ⁻¹. Plant height also varied considerably; in cv. ‘Hyde Park’ the tallest plants (greater than 140 mm) were recorded under treatment with BAP at 50 mg L ⁻¹ without AgNPs, while in cv. ‘Yellow Diamond’, the largest plants (greater than 100 mm) were observed with the application of AgNPs at 100 mg L ⁻¹ without BAP. In terms of physiology, the SPAD index did not show significant improvements compared to the control group. In conclusion, the results indicate that the impact of the interaction between AgNPs and BAP is concentration, cultivar, and measured variable dependent, suggesting a varietalspecific response and the possibility of both beneficial and adverse effects on different aspects of lily development. This underscores the importance of carefully adjusting doses and combinations to optimize regeneration and plant growth.
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spelling INIA28172025-08-14T16:43:30Z Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles Tomanguilla Llanos, Oscar Meléndez Mori, Jegnes Benjamín Tejada Alvarado, José Jesús Lapiz Culqui, Yoiner K. Huaman Huaman, Eyner Zuta Puscan, Marileydi Mego Pérez, Reyna Stefani Oliva Cruz, Manuel bulb scale cytokinin growth regulators nanotechnology propagation escama del bulbo citoquinina reguladores del crecimiento nanotecnología propagación https://purl.org/pe-repo/ocde/ford#4.04.00 Lilie; lirio; Bulb; bulbo; Nanoparticle; nanopartícula; plant biotechnology; biotecnología vegetal; ornamental plant; planta ornamental The lily stands out as one of the most economically relevant flowers worldwide and enjoys great popularity as a cut flower in the florist industry. This prominent position underlines the need to implement and continuously improve sustainable protocols that ensure its efficient production, multiplication and conservation. In this context, the present study aimed to investigate the potential biostimulant effect of Silver Nanoparticles (AgNPs) and 6-Benzylaminopurine (BAP) on two lily cultivars (Lilium LA Hybrid ‘Hyde Park’ and Lilium LA Hybrid ‘Yellow Diamond’), through their individual and combined application. The research focused on evaluating the influence of these treatments on two key aspects: (1) regeneration capacity and bulblet development from bulb scales, and (2) morpho-physiological growth parameters in regenerated plants. Analysis of the results revealed several significant effects of the interaction between AgNPs and BAP on different regeneration and growth parameters in two lily cultivars. In cv. ‘Hyde Park’, the combination of AgNPs at 50 mg L ⁻¹ with BAP at 200 mg L ⁻¹ increased bulblet number to an average of 5.6. In cv. ‘Yellow Diamond’, the application of AgNPs at 50 mg L ⁻¹ without BAP and the combined treatment of AgNPs at 100 mg L ⁻¹ with BAP at 200 mg L ⁻¹ produced a higher number of bulblets (3.2). The highest bulblet weight in both cultivars was obtained with the combination of AgNPs at 100 mg L ⁻¹ and BAP at 50 mg L ⁻¹. Plant height also varied considerably; in cv. ‘Hyde Park’ the tallest plants (greater than 140 mm) were recorded under treatment with BAP at 50 mg L ⁻¹ without AgNPs, while in cv. ‘Yellow Diamond’, the largest plants (greater than 100 mm) were observed with the application of AgNPs at 100 mg L ⁻¹ without BAP. In terms of physiology, the SPAD index did not show significant improvements compared to the control group. In conclusion, the results indicate that the impact of the interaction between AgNPs and BAP is concentration, cultivar, and measured variable dependent, suggesting a varietalspecific response and the possibility of both beneficial and adverse effects on different aspects of lily development. This underscores the importance of carefully adjusting doses and combinations to optimize regeneration and plant growth. 2025-08-14T16:43:30Z 2025-08-14T16:43:30Z 2025-07-01 info:eu-repo/semantics/article Tomanguilla-Llanos, O., Meléndez-Mori, J. B., Tejada-Alvarado, J. J., Lapiz-Culqui, Y. K., Huaman-Huaman, E., Zuta-Puscan, M., Mego-Pérez, R. S., & Oliva-Cruz, M. (2025). Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles. Scientific Reports, 15, 22099. https://doi.org/10.1038/s41598-025-06078-7 2045-2322 http://hdl.handle.net/20.500.12955/2817 https://doi.org/10.1038/s41598-025-06078-7 eng urn:issn:2045-2322 Scientific Reports info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf application/pdf Springer Nature GB Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA
spellingShingle bulb scale
cytokinin
growth regulators
nanotechnology
propagation
escama del bulbo
citoquinina
reguladores del crecimiento
nanotecnología
propagación
https://purl.org/pe-repo/ocde/ford#4.04.00
Lilie; lirio; Bulb; bulbo; Nanoparticle; nanopartícula; plant biotechnology; biotecnología vegetal; ornamental plant; planta ornamental
Tomanguilla Llanos, Oscar
Meléndez Mori, Jegnes Benjamín
Tejada Alvarado, José Jesús
Lapiz Culqui, Yoiner K.
Huaman Huaman, Eyner
Zuta Puscan, Marileydi
Mego Pérez, Reyna Stefani
Oliva Cruz, Manuel
Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles
title Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles
title_full Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles
title_fullStr Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles
title_full_unstemmed Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles
title_short Boosting lily bulblets production: a study on the effects of 6-benzylaminopurine and silver nanoparticles
title_sort boosting lily bulblets production a study on the effects of 6 benzylaminopurine and silver nanoparticles
topic bulb scale
cytokinin
growth regulators
nanotechnology
propagation
escama del bulbo
citoquinina
reguladores del crecimiento
nanotecnología
propagación
https://purl.org/pe-repo/ocde/ford#4.04.00
Lilie; lirio; Bulb; bulbo; Nanoparticle; nanopartícula; plant biotechnology; biotecnología vegetal; ornamental plant; planta ornamental
url http://hdl.handle.net/20.500.12955/2817
https://doi.org/10.1038/s41598-025-06078-7
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