Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation

The objective of this study was to assess the effects of saponins derived from Chenopodium quinoa by-products on methane (CH4) production in a ruminal fermentation system under in vitro conditions. Additionally, the study included the analysis of short-chain fatty acids (SCFAs) and true dry matter d...

Descripción completa

Detalles Bibliográficos
Autores principales: Alegría Arnedo, Maria Cecilia, Gomez Bravo, Carlos Alfredo, Quispe Jacobo, Fredy Enrique
Formato: info:eu-repo/semantics/article
Lenguaje:Inglés
Publicado: GB 2025
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12955/2672
_version_ 1855028819428114432
author Alegría Arnedo, Maria Cecilia
Gomez Bravo, Carlos Alfredo
Quispe Jacobo, Fredy Enrique
author_browse Alegría Arnedo, Maria Cecilia
Gomez Bravo, Carlos Alfredo
Quispe Jacobo, Fredy Enrique
author_facet Alegría Arnedo, Maria Cecilia
Gomez Bravo, Carlos Alfredo
Quispe Jacobo, Fredy Enrique
author_sort Alegría Arnedo, Maria Cecilia
collection Repositorio INIA
description The objective of this study was to assess the effects of saponins derived from Chenopodium quinoa by-products on methane (CH4) production in a ruminal fermentation system under in vitro conditions. Additionally, the study included the analysis of short-chain fatty acids (SCFAs) and true dry matter digestibility (TDMD). The saponin extracts obtained from three quinoa varieties were Altiplano (AS), Quillahuaman (QS), and Salcedo (SS), and were tested at six different doses (0, 0.2, 0.4, 0.6, 0.8, and 1.0mg/mL) each. Rumen fluid was obtained from two fistulated Junín breed sheep, with alfalfa hay (AH) and a 1:1 mixture of maize and bran (MB) was used as basal substrates. The data were processed in a completely randomized design and replicated three times. The CH4 per dry matter (mL/g DM), after 24h of incubation significantly decreased, acetate molar concentration decreased (p<0.05) with AS (3.6%) and QS (6.4%), propionate values increased (P<0.05) to 14.4% (AS), 15.7% (QS), and 15.5% (SS), the acetate-to-propionate ratio decreased (P<0.05) across all saponins treatments compared to the control and irrespective the substrates. The CH4 to TDMD ratio decreased (P<0.05) with all saponins. Methane inhibition (mL CH4/100mgTDMD) was higher by up 25.7% in the presence of AS, followed by QS at 18.7% and SS at 14.6%. The results indicate that saponins extracted from quinoa possess significant potential as feed additives for ruminants. Their application could contribute to the reduction of methane production, which would be advantageous for both livestock efficiency and environmental sustainability.
format info:eu-repo/semantics/article
id INIA2672
institution Institucional Nacional de Innovación Agraria
language Inglés
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher GB
publisherStr GB
record_format dspace
spelling INIA26722025-03-12T20:00:17Z Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation Alegría Arnedo, Maria Cecilia Gomez Bravo, Carlos Alfredo Quispe Jacobo, Fredy Enrique Extracts Quinoa CH4 Ruminal fermentation Substrates https://purl.org/pe-repo/ocde/ford#4.01.01 Chenopodium quinoa | Saponinas | Metano | Fermentación ruminal | Extractos | Substratos | Alimentos para animales The objective of this study was to assess the effects of saponins derived from Chenopodium quinoa by-products on methane (CH4) production in a ruminal fermentation system under in vitro conditions. Additionally, the study included the analysis of short-chain fatty acids (SCFAs) and true dry matter digestibility (TDMD). The saponin extracts obtained from three quinoa varieties were Altiplano (AS), Quillahuaman (QS), and Salcedo (SS), and were tested at six different doses (0, 0.2, 0.4, 0.6, 0.8, and 1.0mg/mL) each. Rumen fluid was obtained from two fistulated Junín breed sheep, with alfalfa hay (AH) and a 1:1 mixture of maize and bran (MB) was used as basal substrates. The data were processed in a completely randomized design and replicated three times. The CH4 per dry matter (mL/g DM), after 24h of incubation significantly decreased, acetate molar concentration decreased (p<0.05) with AS (3.6%) and QS (6.4%), propionate values increased (P<0.05) to 14.4% (AS), 15.7% (QS), and 15.5% (SS), the acetate-to-propionate ratio decreased (P<0.05) across all saponins treatments compared to the control and irrespective the substrates. The CH4 to TDMD ratio decreased (P<0.05) with all saponins. Methane inhibition (mL CH4/100mgTDMD) was higher by up 25.7% in the presence of AS, followed by QS at 18.7% and SS at 14.6%. The results indicate that saponins extracted from quinoa possess significant potential as feed additives for ruminants. Their application could contribute to the reduction of methane production, which would be advantageous for both livestock efficiency and environmental sustainability. 2025-03-12T20:00:16Z 2025-03-12T20:00:16Z 2024-11-01 info:eu-repo/semantics/article Alegría-Arnedo MC, Gómez-Bravo CA and Quispe-Jacobo FE, 2025. Effect of saponins from Chenopodium quinoa willd. on methane production, short chain fatty acids and digestibility in vitro ruminal fermentation. International Journal of Veterinary Science 14(2): 341-350. https://doi.org/10.47278/journal.ijvs/2024.248 http://hdl.handle.net/20.500.12955/2672 10.47278/journal.ijvs/2024.248 eng 2304-3075 International Journal of Veterinary Science info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf GB Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA
spellingShingle Extracts
Quinoa
CH4
Ruminal fermentation
Substrates
https://purl.org/pe-repo/ocde/ford#4.01.01
Chenopodium quinoa | Saponinas | Metano | Fermentación ruminal | Extractos | Substratos | Alimentos para animales
Alegría Arnedo, Maria Cecilia
Gomez Bravo, Carlos Alfredo
Quispe Jacobo, Fredy Enrique
Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation
title Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation
title_full Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation
title_fullStr Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation
title_full_unstemmed Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation
title_short Effect of Saponins from Chenopodium quinoa Willd. on Methane Production, Short Chain Fatty Acids and Digestibility in vitro Ruminal Fermentation
title_sort effect of saponins from chenopodium quinoa willd on methane production short chain fatty acids and digestibility in vitro ruminal fermentation
topic Extracts
Quinoa
CH4
Ruminal fermentation
Substrates
https://purl.org/pe-repo/ocde/ford#4.01.01
Chenopodium quinoa | Saponinas | Metano | Fermentación ruminal | Extractos | Substratos | Alimentos para animales
url http://hdl.handle.net/20.500.12955/2672
work_keys_str_mv AT alegriaarnedomariacecilia effectofsaponinsfromchenopodiumquinoawilldonmethaneproductionshortchainfattyacidsanddigestibilityinvitroruminalfermentation
AT gomezbravocarlosalfredo effectofsaponinsfromchenopodiumquinoawilldonmethaneproductionshortchainfattyacidsanddigestibilityinvitroruminalfermentation
AT quispejacobofredyenrique effectofsaponinsfromchenopodiumquinoawilldonmethaneproductionshortchainfattyacidsanddigestibilityinvitroruminalfermentation