Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru

Background: Colibacillosis caused by Escherichia coli causes significant economic losses in the livestock sector worldwide and is one of the calves’ leading causes of diarrea Aim: This study aimed to identify the most frequent E. coli molecularly pathotypes in calves with diarrhea in six provinces o...

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Autores principales: Cabrera González, Marco, Quilcate Pairazamán, Carlos, Alvarez García, Wuesley Yusmein, Cabrera Hoyos, Héctor, Tayca Saldaña, Antony, Aliaga Tambo, Fernando, Rojas Valdez, Deisy, Cueva Rodríguez, Medali
Formato: info:eu-repo/semantics/article
Lenguaje:Inglés
Publicado: Faculty of Veterinary Medicine, University of Tripoli 2024
Materias:
Acceso en línea:https://hdl.handle.net/20.500.12955/2591
https://doi.org/10.5455/OVJ.2024.v14.i9.6
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author Cabrera González, Marco
Quilcate Pairazamán, Carlos
Alvarez García, Wuesley Yusmein
Cabrera Hoyos, Héctor
Tayca Saldaña, Antony
Aliaga Tambo, Fernando
Rojas Valdez, Deisy
Cueva Rodríguez, Medali
author_browse Aliaga Tambo, Fernando
Alvarez García, Wuesley Yusmein
Cabrera González, Marco
Cabrera Hoyos, Héctor
Cueva Rodríguez, Medali
Quilcate Pairazamán, Carlos
Rojas Valdez, Deisy
Tayca Saldaña, Antony
author_facet Cabrera González, Marco
Quilcate Pairazamán, Carlos
Alvarez García, Wuesley Yusmein
Cabrera Hoyos, Héctor
Tayca Saldaña, Antony
Aliaga Tambo, Fernando
Rojas Valdez, Deisy
Cueva Rodríguez, Medali
author_sort Cabrera González, Marco
collection Repositorio INIA
description Background: Colibacillosis caused by Escherichia coli causes significant economic losses in the livestock sector worldwide and is one of the calves’ leading causes of diarrea Aim: This study aimed to identify the most frequent E. coli molecularly pathotypes in calves with diarrhea in six provinces of the Cajamarca region in the northern highlands of Peru. Methods: Twenty-eight herds of dairy cattle under a semi-intensive rearing system were evaluated; 95 samples were isolated from calves with diarrhea up to the first month of life, 62 males and 33 females, during the rainy season. Results: The presence of virulence genes of E. coli strains was more prevalent in males; the astA (89.47%), st (83.15%), and f5 (57.89%) genes were more expressed, and the lt (17.89%) and stx2 (1.05%) genes were less expressed. The eae gene (21.05%) was more present in females. Conclusion: When E. coli strains express virulence genes astA, st, and f5 and their atypical double, triple, and quadruple combination between different observed pathotypes, they give rise to the formation of several pathotypes by the horizontal transfer of virulence genes, which can cause colibacillosis processes in more virulent calves, which is one of the most important causes of diarrhea in calves in the region of Cajamarca, compromising the sanitary viability in the herds.
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spelling INIA25912025-03-04T21:41:00Z Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru Cabrera González, Marco Quilcate Pairazamán, Carlos Alvarez García, Wuesley Yusmein Cabrera Hoyos, Héctor Tayca Saldaña, Antony Aliaga Tambo, Fernando Rojas Valdez, Deisy Cueva Rodríguez, Medali Calves Colibacillosis E. coli Pathotypes https://purl.org/pe-repo/ocde/ford#4.03.01 Calves Ternero Colibacillosis Colibacilosis Escherichia coli Pathotypes Patotipo Background: Colibacillosis caused by Escherichia coli causes significant economic losses in the livestock sector worldwide and is one of the calves’ leading causes of diarrea Aim: This study aimed to identify the most frequent E. coli molecularly pathotypes in calves with diarrhea in six provinces of the Cajamarca region in the northern highlands of Peru. Methods: Twenty-eight herds of dairy cattle under a semi-intensive rearing system were evaluated; 95 samples were isolated from calves with diarrhea up to the first month of life, 62 males and 33 females, during the rainy season. Results: The presence of virulence genes of E. coli strains was more prevalent in males; the astA (89.47%), st (83.15%), and f5 (57.89%) genes were more expressed, and the lt (17.89%) and stx2 (1.05%) genes were less expressed. The eae gene (21.05%) was more present in females. Conclusion: When E. coli strains express virulence genes astA, st, and f5 and their atypical double, triple, and quadruple combination between different observed pathotypes, they give rise to the formation of several pathotypes by the horizontal transfer of virulence genes, which can cause colibacillosis processes in more virulent calves, which is one of the most important causes of diarrhea in calves in the region of Cajamarca, compromising the sanitary viability in the herds. This research was partially funded by the “Proyecto de Mejoramiento Genético Nacional-PROMEG CUI 2432072”. 2024-10-03T14:01:00Z 2024-10-03T14:01:00Z 2024-09-30 info:eu-repo/semantics/article Cabrera-González, M.; Quilcate-Pairazamán, C.; Álvarez-García, W.; Cabrera-Hoyos, H.; Tayca-Saldaña, A., Aliaga-Tambo, F.; Rojas-Valdez, D. & Cueva-Rodríguez, M. (2024). Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru. Open Veterinary Journal, 14 (9), 2170-2180. doi:10.5455/OVJ.2024.v14.i9.6 2218-6050 https://hdl.handle.net/20.500.12955/2591 https://doi.org/10.5455/OVJ.2024.v14.i9.6 eng urn:issn:2218-6050 Open Veterinary Journal info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/4.0/ application/pdf application/pdf Faculty of Veterinary Medicine, University of Tripoli LY Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA
spellingShingle Calves
Colibacillosis
E. coli
Pathotypes
https://purl.org/pe-repo/ocde/ford#4.03.01
Calves
Ternero
Colibacillosis
Colibacilosis
Escherichia coli
Pathotypes
Patotipo
Cabrera González, Marco
Quilcate Pairazamán, Carlos
Alvarez García, Wuesley Yusmein
Cabrera Hoyos, Héctor
Tayca Saldaña, Antony
Aliaga Tambo, Fernando
Rojas Valdez, Deisy
Cueva Rodríguez, Medali
Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru
title Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru
title_full Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru
title_fullStr Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru
title_full_unstemmed Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru
title_short Molecular identification of the most frequent pathotypes of Escherichia coli in calves with diarrhoea in the Cajamarca region of Peru
title_sort molecular identification of the most frequent pathotypes of escherichia coli in calves with diarrhoea in the cajamarca region of peru
topic Calves
Colibacillosis
E. coli
Pathotypes
https://purl.org/pe-repo/ocde/ford#4.03.01
Calves
Ternero
Colibacillosis
Colibacilosis
Escherichia coli
Pathotypes
Patotipo
url https://hdl.handle.net/20.500.12955/2591
https://doi.org/10.5455/OVJ.2024.v14.i9.6
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