Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas
Low motility and low sperm concentration are characteristics of alpaca semen. Thus, the intracytoplasmic sperm injection (ICSI) technique represents an alternative to improve the reproductive capacity of the male. However, the effect of post-ICSI activation in alpaca is not yet known. The aim of the...
| Autores principales: | , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
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Elsevier
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2471 https://doi.org/10.1016/j.anireprosci.2024.107432 |
| _version_ | 1855028895433097216 |
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| author | Bellido Quispe, Dionet Keny Mujica Lengua, Fidel Rodolfo Contreras Huamani, Mijaíl Palomino, J. Manuel |
| author_browse | Bellido Quispe, Dionet Keny Contreras Huamani, Mijaíl Mujica Lengua, Fidel Rodolfo Palomino, J. Manuel |
| author_facet | Bellido Quispe, Dionet Keny Mujica Lengua, Fidel Rodolfo Contreras Huamani, Mijaíl Palomino, J. Manuel |
| author_sort | Bellido Quispe, Dionet Keny |
| collection | Repositorio INIA |
| description | Low motility and low sperm concentration are characteristics of alpaca semen. Thus, the intracytoplasmic sperm injection (ICSI) technique represents an alternative to improve the reproductive capacity of the male. However, the effect of post-ICSI activation in alpaca is not yet known. The aim of the present study was to compare the effect of chemical activators on alpaca embryo development after ICSI. Alpaca ovaries were collected from a local slaughterhouse and transported to the laboratory. Category I, II and III oocytes were matured for 30 h at 38.5 °C. After ICSI, injected oocytes were randomly divided and activated as follows: i) 5 μM ionomycin for 5 min, ii) 7% ethanol for 4 min, iii) 5 μM ionomycin for 5 min, window period 3 h plus 7% ethanol for 4 min, iv) 5 μM ionomycin for 5 min, window period 3 h, a second ionomycin treatment for 5 min, followed by 1.9 mM 6-DMAP for 3 h, v) 10 mM SrCl2 for 3 h. Culture was carried out for 5 days in SOFaa at 38.5 °C. The cleavage rate was the lowest in the SrCl2 group, morula development was the lowest in the SrCl2 and without activation groups, and blastocyst stage was not different between groups (P<0.05). The rates with SrCl2 were lower in total embryos produced, whereas in transferable embryos they were lower with 2Io/6-DMAP and with SrCl2 (P<0.05). In conclusion, alpaca oocyte activation is more efficient with ionomycin and ethanol to produce transferable embryos. |
| format | info:eu-repo/semantics/article |
| id | INIA2471 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INIA24712024-04-02T17:01:50Z Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas Bellido Quispe, Dionet Keny Mujica Lengua, Fidel Rodolfo Contreras Huamani, Mijaíl Palomino, J. Manuel Oocyte activation Intracytoplasmic sperm injection (ICSI) Embryo development In vitro embryos Alpacas https://purl.org/pe-repo/ocde/ford#4.02.01 Oocytes Ovocitos In vitro embryo production Producción de embriones in vitro Alpacas Alpaca Low motility and low sperm concentration are characteristics of alpaca semen. Thus, the intracytoplasmic sperm injection (ICSI) technique represents an alternative to improve the reproductive capacity of the male. However, the effect of post-ICSI activation in alpaca is not yet known. The aim of the present study was to compare the effect of chemical activators on alpaca embryo development after ICSI. Alpaca ovaries were collected from a local slaughterhouse and transported to the laboratory. Category I, II and III oocytes were matured for 30 h at 38.5 °C. After ICSI, injected oocytes were randomly divided and activated as follows: i) 5 μM ionomycin for 5 min, ii) 7% ethanol for 4 min, iii) 5 μM ionomycin for 5 min, window period 3 h plus 7% ethanol for 4 min, iv) 5 μM ionomycin for 5 min, window period 3 h, a second ionomycin treatment for 5 min, followed by 1.9 mM 6-DMAP for 3 h, v) 10 mM SrCl2 for 3 h. Culture was carried out for 5 days in SOFaa at 38.5 °C. The cleavage rate was the lowest in the SrCl2 group, morula development was the lowest in the SrCl2 and without activation groups, and blastocyst stage was not different between groups (P<0.05). The rates with SrCl2 were lower in total embryos produced, whereas in transferable embryos they were lower with 2Io/6-DMAP and with SrCl2 (P<0.05). In conclusion, alpaca oocyte activation is more efficient with ionomycin and ethanol to produce transferable embryos. 2024-04-02T17:01:48Z 2024-04-02T17:01:48Z 2024-02-14 info:eu-repo/semantics/article Bellido-Quispe, D. K.; Lengua, F. R. M.; Huamani, M. C.; & Palomino, J. M. (2024). Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas. Animal Reproduction Science, 263, 107432. doi: 10.1016/j.anireprosci.2024.107432 1873-2232 https://hdl.handle.net/20.500.12955/2471 https://doi.org/10.1016/j.anireprosci.2024.107432 eng urn:issn:1873-2232 Animal Reproduction Science info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf application/pdf Elsevier NL Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Oocyte activation Intracytoplasmic sperm injection (ICSI) Embryo development In vitro embryos Alpacas https://purl.org/pe-repo/ocde/ford#4.02.01 Oocytes Ovocitos In vitro embryo production Producción de embriones in vitro Alpacas Alpaca Bellido Quispe, Dionet Keny Mujica Lengua, Fidel Rodolfo Contreras Huamani, Mijaíl Palomino, J. Manuel Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas |
| title | Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas |
| title_full | Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas |
| title_fullStr | Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas |
| title_full_unstemmed | Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas |
| title_short | Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas |
| title_sort | effect of chemical activators after intracytoplasmic sperm injection icsi on embryo development in alpacas |
| topic | Oocyte activation Intracytoplasmic sperm injection (ICSI) Embryo development In vitro embryos Alpacas https://purl.org/pe-repo/ocde/ford#4.02.01 Oocytes Ovocitos In vitro embryo production Producción de embriones in vitro Alpacas Alpaca |
| url | https://hdl.handle.net/20.500.12955/2471 https://doi.org/10.1016/j.anireprosci.2024.107432 |
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