Predictive characteristics of male fertility in alpacas with special reference to seminal NGF
Recent studies document the LH-releasing pathway of nerve growth factor (NGF) in male camelids and that the LH response to seminal NGF is associated with elevated plasma testosterone concentration. Results provide rationale for the hypothesis that NGF in semen is associated with male fertility. In E...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/workingPaper |
| Language: | Inglés |
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Elsevier
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12955/2430 https://doi.org/10.1016/j.theriogenology.2023.12.033 |
| _version_ | 1855028827552481280 |
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| author | Carrasco, R. A. Bogle, O. A. Ratto, M. H. Ulloa Leal, C. Mamani Cato, Rubén Herberht Adams, Gregg Patrick |
| author_browse | Adams, Gregg Patrick Bogle, O. A. Carrasco, R. A. Mamani Cato, Rubén Herberht Ratto, M. H. Ulloa Leal, C. |
| author_facet | Carrasco, R. A. Bogle, O. A. Ratto, M. H. Ulloa Leal, C. Mamani Cato, Rubén Herberht Adams, Gregg Patrick |
| author_sort | Carrasco, R. A. |
| collection | Repositorio INIA |
| description | Recent studies document the LH-releasing pathway of nerve growth factor (NGF) in male camelids and that the LH response to seminal NGF is associated with elevated plasma testosterone concentration. Results provide rationale for the hypothesis that NGF in semen is associated with male fertility. In Experiment 1, the association between the amount of NGF in the ejaculate and characteristics of the male reproductive system was examined in alpacas. The concentration of NGF was measured by radioimmunoassay in semen samples collected from male alpacas (n = 47) and correlated with sperm morphology and motility, and measurements of the male reproductive anatomy. Most ejaculates had NGF concentrations that, based on previous studies, triggered ovulation in female camelids, however, we only found a positive correlation between NGF concentration with sperm concentration, thread formation and total NGF, and a negative correlation with pH. In Experiment 2, a retrospective analysis was carried out to determine if breeding performance during the previous season was related to recent concentrations of seminal NGF in male alpacas (n = 22). Birth rates tended to be correlated with sperm concentration and total amount of NGF in the ejaculate (P = 0.09). Experiment 3 was a prospective study to determine the relationship between seminal NGF (n = 8 male alpacas) and ovulation and pregnancy rates in a breeding trial. No association was detected between seminal NGF concentration and ovulation rate, pregnancy rate, or LH response in the female. We conclude that among the breeding males used in our study, the abundance of seminal NGF was correlated with sperm concentration and thread formation, however, it was not predictive of male fertility in alpacas. Examination of males not previously selected as breeding stock may be expected to include a broader range of seminal NGF and provide a more comprehensive understanding of the relationship between seminal NGF and male fertility. |
| format | info:eu-repo/semantics/workingPaper |
| id | INIA2430 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INIA24302024-11-29T03:34:54Z Predictive characteristics of male fertility in alpacas with special reference to seminal NGF Carrasco, R. A. Bogle, O. A. Ratto, M. H. Ulloa Leal, C. Mamani Cato, Rubén Herberht Adams, Gregg Patrick Alpaca Semen NGF Fertility https://purl.org/pe-repo/ocde/ford#4.02.01 Alpaca Alpacas Semen Fertility Fertilidad Recent studies document the LH-releasing pathway of nerve growth factor (NGF) in male camelids and that the LH response to seminal NGF is associated with elevated plasma testosterone concentration. Results provide rationale for the hypothesis that NGF in semen is associated with male fertility. In Experiment 1, the association between the amount of NGF in the ejaculate and characteristics of the male reproductive system was examined in alpacas. The concentration of NGF was measured by radioimmunoassay in semen samples collected from male alpacas (n = 47) and correlated with sperm morphology and motility, and measurements of the male reproductive anatomy. Most ejaculates had NGF concentrations that, based on previous studies, triggered ovulation in female camelids, however, we only found a positive correlation between NGF concentration with sperm concentration, thread formation and total NGF, and a negative correlation with pH. In Experiment 2, a retrospective analysis was carried out to determine if breeding performance during the previous season was related to recent concentrations of seminal NGF in male alpacas (n = 22). Birth rates tended to be correlated with sperm concentration and total amount of NGF in the ejaculate (P = 0.09). Experiment 3 was a prospective study to determine the relationship between seminal NGF (n = 8 male alpacas) and ovulation and pregnancy rates in a breeding trial. No association was detected between seminal NGF concentration and ovulation rate, pregnancy rate, or LH response in the female. We conclude that among the breeding males used in our study, the abundance of seminal NGF was correlated with sperm concentration and thread formation, however, it was not predictive of male fertility in alpacas. Examination of males not previously selected as breeding stock may be expected to include a broader range of seminal NGF and provide a more comprehensive understanding of the relationship between seminal NGF and male fertility. 2024-02-09T17:47:39Z 2024-02-09T17:47:39Z 2023-12-29 2024-12-29 info:eu-repo/semantics/workingPaper Carrasco, R.; Bogle, O.; Ratto, M.; Ulloa-Leal, C.; Mamani-Cato, R.; & Adams, G. (2024). Predictive characteristics of male fertility in alpacas with special reference to seminal NGF. Theriogenology. doi: 10.1016/j.theriogenology.2023.12.033. 1879-3231 https://hdl.handle.net/20.500.12955/2430 https://doi.org/10.1016/j.theriogenology.2023.12.033 eng urn:issn:1879-3231 Theriogenology info:eu-repo/semantics/embargoedAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf application/pdf Elsevier US Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Alpaca Semen NGF Fertility https://purl.org/pe-repo/ocde/ford#4.02.01 Alpaca Alpacas Semen Fertility Fertilidad Carrasco, R. A. Bogle, O. A. Ratto, M. H. Ulloa Leal, C. Mamani Cato, Rubén Herberht Adams, Gregg Patrick Predictive characteristics of male fertility in alpacas with special reference to seminal NGF |
| title | Predictive characteristics of male fertility in alpacas with special reference to seminal NGF |
| title_full | Predictive characteristics of male fertility in alpacas with special reference to seminal NGF |
| title_fullStr | Predictive characteristics of male fertility in alpacas with special reference to seminal NGF |
| title_full_unstemmed | Predictive characteristics of male fertility in alpacas with special reference to seminal NGF |
| title_short | Predictive characteristics of male fertility in alpacas with special reference to seminal NGF |
| title_sort | predictive characteristics of male fertility in alpacas with special reference to seminal ngf |
| topic | Alpaca Semen NGF Fertility https://purl.org/pe-repo/ocde/ford#4.02.01 Alpaca Alpacas Semen Fertility Fertilidad |
| url | https://hdl.handle.net/20.500.12955/2430 https://doi.org/10.1016/j.theriogenology.2023.12.033 |
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