Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders
The aim of this study was to evaluate alpaca pregnancy outcomes and birth rates of females inseminated with frozen semen using two commercial extenders. A total of 18 ejaculates from 8 adult alpaca males were obtained with artificial vagina, and macroscopic and microscopic semen characteristics were...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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John Wiley & Sons Inc.
2024
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| Online Access: | https://hdl.handle.net/20.500.12955/2416 https://doi.org/10.1111/rda.14514 |
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| author | Contreras Huamani, Mijaíl Olaguivel Flores, C. A. Guillén Palomino, Crissthel Yverlin Carretero, M. I. Fumuso, F. G. Laines Arcce, I. M. |
| author_browse | Carretero, M. I. Contreras Huamani, Mijaíl Fumuso, F. G. Guillén Palomino, Crissthel Yverlin Laines Arcce, I. M. Olaguivel Flores, C. A. |
| author_facet | Contreras Huamani, Mijaíl Olaguivel Flores, C. A. Guillén Palomino, Crissthel Yverlin Carretero, M. I. Fumuso, F. G. Laines Arcce, I. M. |
| author_sort | Contreras Huamani, Mijaíl |
| collection | Repositorio INIA |
| description | The aim of this study was to evaluate alpaca pregnancy outcomes and birth rates of females inseminated with frozen semen using two commercial extenders. A total of 18 ejaculates from 8 adult alpaca males were obtained with artificial vagina, and macroscopic and microscopic semen characteristics were assessed. Afterwards, samples were divided into two aliquots, diluted with Biladyl® B or AndroMed®, and cooled for 2 h at 5°C. At that moment, sperm motility was evaluated, and samples were frozen through a gradual descent of temperature using a liquid nitrogen tank. To analyse frozen sperm quality, samples were thawed at 38°C for 30 s. Even though a significant decrease in sperm motility and viability was detected when thawed (p < .05), no superiority was found between the two commercial extenders (Biladyl® B vs. AndroMed®). A total of 36 alpaca females were artificially inseminated (AI) between 30 and 34 h post-injection of a GnRH analogue, administered when a growing dominant follicle was detected through transrectal palpation and ultrasonography. Obtained pregnancy rates were similar between Biladyl® B (33.3%, 6/18) and AndroMed® (22.2%, 4/18). No significant differences were detected in birth rates between the two tested extenders, obtaining 4 and 3 births for Biladyl® and AndroMed®, respectively. In conclusion, alpaca pregnancies and alive offspring can be obtained through AI with frozen semen at similar efficiency rates using commercial diluents, Biladyl® B or AndroMed®. |
| format | info:eu-repo/semantics/article |
| id | INIA2416 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | John Wiley & Sons Inc. |
| publisherStr | John Wiley & Sons Inc. |
| record_format | dspace |
| spelling | INIA24162024-02-28T19:12:47Z Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders Contreras Huamani, Mijaíl Olaguivel Flores, C. A. Guillén Palomino, Crissthel Yverlin Carretero, M. I. Fumuso, F. G. Laines Arcce, I. M. Alpaca AndroMed® Biladyl® B Birth rates Pregnancy Semen cryopreservation https://purl.org/pe-repo/ocde/ford#4.02.01 Alpaca Tasa de natalidad Gestación Conservación del semen Alpacas Birth rate Pregnancy Semen preservation The aim of this study was to evaluate alpaca pregnancy outcomes and birth rates of females inseminated with frozen semen using two commercial extenders. A total of 18 ejaculates from 8 adult alpaca males were obtained with artificial vagina, and macroscopic and microscopic semen characteristics were assessed. Afterwards, samples were divided into two aliquots, diluted with Biladyl® B or AndroMed®, and cooled for 2 h at 5°C. At that moment, sperm motility was evaluated, and samples were frozen through a gradual descent of temperature using a liquid nitrogen tank. To analyse frozen sperm quality, samples were thawed at 38°C for 30 s. Even though a significant decrease in sperm motility and viability was detected when thawed (p < .05), no superiority was found between the two commercial extenders (Biladyl® B vs. AndroMed®). A total of 36 alpaca females were artificially inseminated (AI) between 30 and 34 h post-injection of a GnRH analogue, administered when a growing dominant follicle was detected through transrectal palpation and ultrasonography. Obtained pregnancy rates were similar between Biladyl® B (33.3%, 6/18) and AndroMed® (22.2%, 4/18). No significant differences were detected in birth rates between the two tested extenders, obtaining 4 and 3 births for Biladyl® and AndroMed®, respectively. In conclusion, alpaca pregnancies and alive offspring can be obtained through AI with frozen semen at similar efficiency rates using commercial diluents, Biladyl® B or AndroMed®. 2024-01-24T20:18:42Z 2024-01-24T20:18:42Z 2023-12-06 info:eu-repo/semantics/article Huamani, M. C.; Olaguivel, C. A.; Palomino, C. G.; Carretero, M. I.; Fumuso, F. G.; & Arcce, I. L. (2023). Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders. Reproduction in Domestic Animals, 00, 1-7. doi: 10.1111/rda.14514 1439-0531 https://hdl.handle.net/20.500.12955/2416 https://doi.org/10.1111/rda.14514 eng Reproduction in Domestic Animals info:eu-repo/semantics/restrictedAccess application/pdf John Wiley & Sons Inc. GB Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Alpaca AndroMed® Biladyl® B Birth rates Pregnancy Semen cryopreservation https://purl.org/pe-repo/ocde/ford#4.02.01 Alpaca Tasa de natalidad Gestación Conservación del semen Alpacas Birth rate Pregnancy Semen preservation Contreras Huamani, Mijaíl Olaguivel Flores, C. A. Guillén Palomino, Crissthel Yverlin Carretero, M. I. Fumuso, F. G. Laines Arcce, I. M. Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders |
| title | Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders |
| title_full | Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders |
| title_fullStr | Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders |
| title_full_unstemmed | Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders |
| title_short | Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders |
| title_sort | pregnancy and birth rate outcomes in alpacas vicugna pacos inseminated with frozen semen using two commercial extenders |
| topic | Alpaca AndroMed® Biladyl® B Birth rates Pregnancy Semen cryopreservation https://purl.org/pe-repo/ocde/ford#4.02.01 Alpaca Tasa de natalidad Gestación Conservación del semen Alpacas Birth rate Pregnancy Semen preservation |
| url | https://hdl.handle.net/20.500.12955/2416 https://doi.org/10.1111/rda.14514 |
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