Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae
Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an economically important pest of variety of crops, including maize (Zea mays L.), rice (Oryza sativa L.), and cotton (Gossypium spp.). Conventional chemical insecticides are usually used for management of this pest; however, a viable al...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Georgia Entomological Society
2023
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| Acceso en línea: | https://hdl.handle.net/20.500.12955/2294 https://doi.org/10.18474/JES22-48 |
| _version_ | 1855490590631788544 |
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| author | Pérez Tesén, Edgar Darwin Rodas Adrianzén, Jennifher Elizabeth Chávez Cabrera, Alexander Neira de Perales, María Elena |
| author_browse | Chávez Cabrera, Alexander Neira de Perales, María Elena Pérez Tesén, Edgar Darwin Rodas Adrianzén, Jennifher Elizabeth |
| author_facet | Pérez Tesén, Edgar Darwin Rodas Adrianzén, Jennifher Elizabeth Chávez Cabrera, Alexander Neira de Perales, María Elena |
| author_sort | Pérez Tesén, Edgar Darwin |
| collection | Repositorio INIA |
| description | Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an economically important pest of variety of crops, including maize (Zea mays L.), rice (Oryza sativa L.), and cotton (Gossypium spp.). Conventional chemical insecticides are usually used for management of this pest; however, a viable alternative is the use of microbial agents or their biological products, such as entomopathogenic nematodes. Their efficacy, however, is affected by abiotic conditions including ultraviolet (UV) light and desiccation. It is therefore necessary to develop formulations that preserve or extend the viability of the agent or product while facilitating ease of application. This laboratory study was conducted to determine the pathogenicity of the nematode Heterorhabditis bacteriophora (Poinar) against S. frugiperda third-instar larvae. Infective juveniles (IJs) of the nematode were tested at a concentration of 350 IJs/ml in two substrates (water and 0.1% [w/v] agar suspension) with four periods of exposure (0, 5, 10 and 15 min) to UV radiation at 253.7 nm. Each of the eight treatments included 40 larvae, with each treatment replicated three times in a completely randomized design (two factors of type of medium and UV exposure time). We determined that the 0.1% (w/v) agar suspension, compared with the water substrate, protected the IJs from harmful UV light and thus improved the survival and pathogenicity of the IJs against third-instar S. frugiperda. |
| format | Artículo |
| id | INIA2294 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Georgia Entomological Society |
| publisherStr | Georgia Entomological Society |
| record_format | dspace |
| spelling | INIA22942023-10-02T17:04:02Z Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae Pérez Tesén, Edgar Darwin Rodas Adrianzén, Jennifher Elizabeth Chávez Cabrera, Alexander Neira de Perales, María Elena Biological control Entomopathogenic nematode Spodoptera frugiperda Ultraviolet radiation https://purl.org/pe-repo/ocde/ford#4.01.06 Biological control Control biológico Entomopathogenic nematode Nematodos entomófilos Spodoptera frugiperda Ultraviolet radiation Radiación ultravioleta Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an economically important pest of variety of crops, including maize (Zea mays L.), rice (Oryza sativa L.), and cotton (Gossypium spp.). Conventional chemical insecticides are usually used for management of this pest; however, a viable alternative is the use of microbial agents or their biological products, such as entomopathogenic nematodes. Their efficacy, however, is affected by abiotic conditions including ultraviolet (UV) light and desiccation. It is therefore necessary to develop formulations that preserve or extend the viability of the agent or product while facilitating ease of application. This laboratory study was conducted to determine the pathogenicity of the nematode Heterorhabditis bacteriophora (Poinar) against S. frugiperda third-instar larvae. Infective juveniles (IJs) of the nematode were tested at a concentration of 350 IJs/ml in two substrates (water and 0.1% [w/v] agar suspension) with four periods of exposure (0, 5, 10 and 15 min) to UV radiation at 253.7 nm. Each of the eight treatments included 40 larvae, with each treatment replicated three times in a completely randomized design (two factors of type of medium and UV exposure time). We determined that the 0.1% (w/v) agar suspension, compared with the water substrate, protected the IJs from harmful UV light and thus improved the survival and pathogenicity of the IJs against third-instar S. frugiperda. 2023-10-02T17:04:00Z 2023-10-02T17:04:00Z 2023-09-08 info:eu-repo/semantics/article Perez, E., Rodas, J.; Cabrera, A.; Neira, M. (2023). Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae. Journal of Entomological Science, 58(4), 412-422. doi: 10.18474/JES22-48 0749-8004 https://hdl.handle.net/20.500.12955/2294 https://doi.org/10.18474/JES22-48 eng urn:issn:0749-8004 Journal of Entomological Science info:eu-repo/semantics/restrictedAccess application/pdf Georgia Entomological Society US Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Biological control Entomopathogenic nematode Spodoptera frugiperda Ultraviolet radiation https://purl.org/pe-repo/ocde/ford#4.01.06 Biological control Control biológico Entomopathogenic nematode Nematodos entomófilos Spodoptera frugiperda Ultraviolet radiation Radiación ultravioleta Pérez Tesén, Edgar Darwin Rodas Adrianzén, Jennifher Elizabeth Chávez Cabrera, Alexander Neira de Perales, María Elena Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae |
| title | Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae |
| title_full | Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae |
| title_fullStr | Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae |
| title_full_unstemmed | Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae |
| title_short | Pathogenicity of Heterorhabditis bacteriophora in ultraviolet light–irradiated agar suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae |
| title_sort | pathogenicity of heterorhabditis bacteriophora in ultraviolet light irradiated agar suspension on spodoptera frugiperda lepidoptera noctuidae larvae |
| topic | Biological control Entomopathogenic nematode Spodoptera frugiperda Ultraviolet radiation https://purl.org/pe-repo/ocde/ford#4.01.06 Biological control Control biológico Entomopathogenic nematode Nematodos entomófilos Spodoptera frugiperda Ultraviolet radiation Radiación ultravioleta |
| url | https://hdl.handle.net/20.500.12955/2294 https://doi.org/10.18474/JES22-48 |
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