Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings
Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or...
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| Format: | Artículo |
| Language: | Inglés |
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Universidad Nacional de Trujillo
2023
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| Online Access: | https://hdl.handle.net/20.500.12955/2235 https://doi.org/10.17268/sci.agropecu.2023.023 |
| _version_ | 1855490628644765696 |
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| author | Hernández Amasifuen, Angel David Rivadeneyra Chisquipama, Lenin Padilla Dominguez, Amner Paredes Espinosa, Richard Guerrero Abad, Juan Carlos Torres Flores, Elías Peláez Rivera, Jorge Luis Carvajal Vallejos, Fernando Marcelo Gutiérrez Reynoso, Dina Lida Corazon Guivin, Mike Anderson |
| author_browse | Carvajal Vallejos, Fernando Marcelo Corazon Guivin, Mike Anderson Guerrero Abad, Juan Carlos Gutiérrez Reynoso, Dina Lida Hernández Amasifuen, Angel David Padilla Dominguez, Amner Paredes Espinosa, Richard Peláez Rivera, Jorge Luis Rivadeneyra Chisquipama, Lenin Torres Flores, Elías |
| author_facet | Hernández Amasifuen, Angel David Rivadeneyra Chisquipama, Lenin Padilla Dominguez, Amner Paredes Espinosa, Richard Guerrero Abad, Juan Carlos Torres Flores, Elías Peláez Rivera, Jorge Luis Carvajal Vallejos, Fernando Marcelo Gutiérrez Reynoso, Dina Lida Corazon Guivin, Mike Anderson |
| author_sort | Hernández Amasifuen, Angel David |
| collection | Repositorio INIA |
| description | Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the multiplication process of H. vastatrix uredospores, using controlled conditions (23±1 °C, ≈ 80% relative humidity and photoperiod of 16 hours of light) under an innovative system, the first symptoms of chlorosis were observed on coffee leaves 20 days after inoculation. Our findings show a 100% incidence of inoculated coffee plants with an average of 54 mg of H. vastatrix uredospores per plant and a 1.38 x 10-1 mg/cm2 sporulation rate. Finally, here we report a protocol that allows mass multiplication of H. vastatrix uredospores under controlled conditions, that would be useful in any time for coffee leaf rust bioassays. |
| format | Artículo |
| id | INIA2235 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Universidad Nacional de Trujillo |
| publisherStr | Universidad Nacional de Trujillo |
| record_format | dspace |
| spelling | INIA22352023-08-21T21:39:11Z Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings Hernández Amasifuen, Angel David Rivadeneyra Chisquipama, Lenin Padilla Dominguez, Amner Paredes Espinosa, Richard Guerrero Abad, Juan Carlos Torres Flores, Elías Peláez Rivera, Jorge Luis Carvajal Vallejos, Fernando Marcelo Gutiérrez Reynoso, Dina Lida Corazon Guivin, Mike Anderson Hemileia vastatrix Coffea arabica Coffee leaf rust Sporulation Uredospores Viability Inoculation https://purl.org/pe-repo/ocde/ford#4.01.07 Hemileia vastatrix Coffea arabica Coffee Café Seedlings Plántulas Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the multiplication process of H. vastatrix uredospores, using controlled conditions (23±1 °C, ≈ 80% relative humidity and photoperiod of 16 hours of light) under an innovative system, the first symptoms of chlorosis were observed on coffee leaves 20 days after inoculation. Our findings show a 100% incidence of inoculated coffee plants with an average of 54 mg of H. vastatrix uredospores per plant and a 1.38 x 10-1 mg/cm2 sporulation rate. Finally, here we report a protocol that allows mass multiplication of H. vastatrix uredospores under controlled conditions, that would be useful in any time for coffee leaf rust bioassays. 2023-07-20T18:00:01Z 2023-07-20T18:00:01Z 2023-07-10 info:eu-repo/semantics/article Hernández-Amasifuen, A. D.; Rivadeneyra-Chisquipama, L.; Padilla-Dominguez, A.; Paredes-Espinosa, R.; Guerrero-Abad, J. C.; Torres-Flores, E.; Peláez-Rivera, J. L.; Carvajal-Vallejos, F. M.; Gutiérrez-Reynoso, D. L.; & Corazon-Guivin, M. A. (2023). Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings. Scientia Agropecuaria, 14(2), 259-267. doi: 10.17268/sci.agropecu.2023.023 2306-6741 https://hdl.handle.net/20.500.12955/2235 https://doi.org/10.17268/sci.agropecu.2023.023 eng urn:issn:2306-6741 Scientia agropecuaria info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/4.0/ application/pdf application/pdf Universidad Nacional de Trujillo PE Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Hemileia vastatrix Coffea arabica Coffee leaf rust Sporulation Uredospores Viability Inoculation https://purl.org/pe-repo/ocde/ford#4.01.07 Hemileia vastatrix Coffea arabica Coffee Café Seedlings Plántulas Hernández Amasifuen, Angel David Rivadeneyra Chisquipama, Lenin Padilla Dominguez, Amner Paredes Espinosa, Richard Guerrero Abad, Juan Carlos Torres Flores, Elías Peláez Rivera, Jorge Luis Carvajal Vallejos, Fernando Marcelo Gutiérrez Reynoso, Dina Lida Corazon Guivin, Mike Anderson Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings |
| title | Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings |
| title_full | Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings |
| title_fullStr | Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings |
| title_full_unstemmed | Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings |
| title_short | Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings |
| title_sort | massive production of hemileia vastatrix uredospores for infection of coffea arabica seedlings |
| topic | Hemileia vastatrix Coffea arabica Coffee leaf rust Sporulation Uredospores Viability Inoculation https://purl.org/pe-repo/ocde/ford#4.01.07 Hemileia vastatrix Coffea arabica Coffee Café Seedlings Plántulas |
| url | https://hdl.handle.net/20.500.12955/2235 https://doi.org/10.17268/sci.agropecu.2023.023 |
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