Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas?
In alpacas (Vicugna pacos), the high cost of in vitro embryo production is also a consequence of the use of several substances in the culture medium. In addition, embryo production rates in this species are still considered low. Thus, in attempt to reduce the cost and to improve the in vitro embryo...
| Autores principales: | , , , , , |
|---|---|
| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Español |
| Publicado: |
Springer
2023
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2234 https://doi.org/10.1007/s11250-023-03646-9 |
| _version_ | 1855028879619522560 |
|---|---|
| author | Contreras Huamani, Mijail Guillén Palomino, Crissthel Yverlin Laines Arcce, Irving Mitchell Silva Chaves, Maiana Magalhães Melo, Luciana De Figueirêdo Freitas, Vicente José |
| author_browse | Contreras Huamani, Mijail De Figueirêdo Freitas, Vicente José Guillén Palomino, Crissthel Yverlin Laines Arcce, Irving Mitchell Magalhães Melo, Luciana Silva Chaves, Maiana |
| author_facet | Contreras Huamani, Mijail Guillén Palomino, Crissthel Yverlin Laines Arcce, Irving Mitchell Silva Chaves, Maiana Magalhães Melo, Luciana De Figueirêdo Freitas, Vicente José |
| author_sort | Contreras Huamani, Mijail |
| collection | Repositorio INIA |
| description | In alpacas (Vicugna pacos), the high cost of in vitro embryo production is also a consequence of the use of several substances in the culture medium. In addition, embryo production rates in this species are still considered low. Thus, in attempt to reduce the cost and to improve the in vitro embryo production rates, this study evaluates the effect of adding follicular fluid (FF) in the in vitro maturation medium on oocyte maturation and subsequent embryo production. After ovary collection at the local slaughterhouse, the oocytes were recovered, selected, and allocated in experimental groups: standard maturation medium (G1) and simplified medium added by 10% FF (G2). The FF was acquired from follicles between 7- and 12-mm diameter. The cumulus cell expansion and the embryo production rates were analyzed by chi-square with p < 0.05. No differences (p > 0.05) were observed in maturation rate between G1 (66.36%) and G2 (63.12%) groups. Likewise, no significant difference (p > 0.05) was verified between G1 and G2 for morula (40.85 vs 38.45%), blastocyst (7.01 vs 6.93%), and total number of embryos (47.87 vs 45.38%). In conclusion, it was possible to simplify the medium used for in vitro maturation of alpaca oocytes resulting in embryo production rates similar to the standard medium. |
| format | info:eu-repo/semantics/article |
| id | INIA2234 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Español |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INIA22342024-02-28T17:30:53Z Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? Contreras Huamani, Mijail Guillén Palomino, Crissthel Yverlin Laines Arcce, Irving Mitchell Silva Chaves, Maiana Magalhães Melo, Luciana De Figueirêdo Freitas, Vicente José Alpaca Cumulus–oocyte complex Oocyte maturation Embryo production https://purl.org/pe-repo/ocde/ford#4.02.01 Oocytes Biological development Ovocitos Lama pacos Vicugna pacos Maduración In alpacas (Vicugna pacos), the high cost of in vitro embryo production is also a consequence of the use of several substances in the culture medium. In addition, embryo production rates in this species are still considered low. Thus, in attempt to reduce the cost and to improve the in vitro embryo production rates, this study evaluates the effect of adding follicular fluid (FF) in the in vitro maturation medium on oocyte maturation and subsequent embryo production. After ovary collection at the local slaughterhouse, the oocytes were recovered, selected, and allocated in experimental groups: standard maturation medium (G1) and simplified medium added by 10% FF (G2). The FF was acquired from follicles between 7- and 12-mm diameter. The cumulus cell expansion and the embryo production rates were analyzed by chi-square with p < 0.05. No differences (p > 0.05) were observed in maturation rate between G1 (66.36%) and G2 (63.12%) groups. Likewise, no significant difference (p > 0.05) was verified between G1 and G2 for morula (40.85 vs 38.45%), blastocyst (7.01 vs 6.93%), and total number of embryos (47.87 vs 45.38%). In conclusion, it was possible to simplify the medium used for in vitro maturation of alpaca oocytes resulting in embryo production rates similar to the standard medium. 2023-07-18T19:48:01Z 2023-07-18T19:48:01Z 2023-06-07 info:eu-repo/semantics/article 0049-4747 https://hdl.handle.net/20.500.12955/2234 https://doi.org/10.1007/s11250-023-03646-9 spa Tropical Animal Health and Production info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Springer GB Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Alpaca Cumulus–oocyte complex Oocyte maturation Embryo production https://purl.org/pe-repo/ocde/ford#4.02.01 Oocytes Biological development Ovocitos Lama pacos Vicugna pacos Maduración Contreras Huamani, Mijail Guillén Palomino, Crissthel Yverlin Laines Arcce, Irving Mitchell Silva Chaves, Maiana Magalhães Melo, Luciana De Figueirêdo Freitas, Vicente José Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? |
| title | Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? |
| title_full | Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? |
| title_fullStr | Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? |
| title_full_unstemmed | Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? |
| title_short | Does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas? |
| title_sort | does the addition of follicular fluid in the in vitro maturation medium increase the oocyte maturation and embryo production in alpacas |
| topic | Alpaca Cumulus–oocyte complex Oocyte maturation Embryo production https://purl.org/pe-repo/ocde/ford#4.02.01 Oocytes Biological development Ovocitos Lama pacos Vicugna pacos Maduración |
| url | https://hdl.handle.net/20.500.12955/2234 https://doi.org/10.1007/s11250-023-03646-9 |
| work_keys_str_mv | AT contrerashuamanimijail doestheadditionoffollicularfluidintheinvitromaturationmediumincreasetheoocytematurationandembryoproductioninalpacas AT guillenpalominocrissthelyverlin doestheadditionoffollicularfluidintheinvitromaturationmediumincreasetheoocytematurationandembryoproductioninalpacas AT lainesarcceirvingmitchell doestheadditionoffollicularfluidintheinvitromaturationmediumincreasetheoocytematurationandembryoproductioninalpacas AT silvachavesmaiana doestheadditionoffollicularfluidintheinvitromaturationmediumincreasetheoocytematurationandembryoproductioninalpacas AT magalhaesmeloluciana doestheadditionoffollicularfluidintheinvitromaturationmediumincreasetheoocytematurationandembryoproductioninalpacas AT defigueiredofreitasvicentejose doestheadditionoffollicularfluidintheinvitromaturationmediumincreasetheoocytematurationandembryoproductioninalpacas |