Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis
In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca e...
| Autores principales: | , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
Mary Ann Liebert, Inc.
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2177 https://doi.org/10.1089/bio.2018.0021 |
| _version_ | 1855028859931459584 |
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| author | Mamani Mango, Guiulfo Moina Gonzales, Milagros Ramos Hidalgo, Martín Mendoza Mallma, José Ruiz Béjar, Jaime Rivas Palma, Victoria Esther Mellisho Salas, Edwin |
| author_browse | Mamani Mango, Guiulfo Mellisho Salas, Edwin Mendoza Mallma, José Moina Gonzales, Milagros Ramos Hidalgo, Martín Rivas Palma, Victoria Esther Ruiz Béjar, Jaime |
| author_facet | Mamani Mango, Guiulfo Moina Gonzales, Milagros Ramos Hidalgo, Martín Mendoza Mallma, José Ruiz Béjar, Jaime Rivas Palma, Victoria Esther Mellisho Salas, Edwin |
| author_sort | Mamani Mango, Guiulfo |
| collection | Repositorio INIA |
| description | In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca epididymis with two methods (flushing and mincing), and to evaluate the in vitro fertilization (IVF) capacity of frozen sperm selected with two different selection methods (washing and swim-up). Sperm samples were processed with Tris-egg yolk or Bioxcell® extenders and frozen with slow freezing and fast freezing. The oocytes were coincubated with spermatozoa for 72 hours, and cleavage rates were recorded afterward. The results indicated that the recovery method did not influence sperm quality (∼70%). However, total sperm recovery was significantly lower for the flushing method than the mincing method. The sperm quality was influenced by the freezing extender (23.3% vs. 33.2%) and freezing rate (20.9% vs. 35.7%). When comparing different methods of sperm selection for IVF, no differences were observed on cleavage rate except for the fact that the concentration of sperm from swim-up method (20.6%) was significantly lower than the one obtained from the washing method (78.7%). The recovery technique of sperm does not affect sperm quality and the method of fast freezing was shown to be the most effective for cryopreservation of alpaca sperm. |
| format | info:eu-repo/semantics/article |
| id | INIA2177 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Mary Ann Liebert, Inc. |
| publisherStr | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | INIA21772023-09-22T03:06:29Z Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis Mamani Mango, Guiulfo Moina Gonzales, Milagros Ramos Hidalgo, Martín Mendoza Mallma, José Ruiz Béjar, Jaime Rivas Palma, Victoria Esther Mellisho Salas, Edwin Cryopreservation Alpaca Spermatozoa Epididymis https://purl.org/pe-repo/ocde/ford#4.02.01 Cryopreservation Alpaca Epididymis Criopreservación Alpacas Spermatozoa Espermatozoo Epididimo In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca epididymis with two methods (flushing and mincing), and to evaluate the in vitro fertilization (IVF) capacity of frozen sperm selected with two different selection methods (washing and swim-up). Sperm samples were processed with Tris-egg yolk or Bioxcell® extenders and frozen with slow freezing and fast freezing. The oocytes were coincubated with spermatozoa for 72 hours, and cleavage rates were recorded afterward. The results indicated that the recovery method did not influence sperm quality (∼70%). However, total sperm recovery was significantly lower for the flushing method than the mincing method. The sperm quality was influenced by the freezing extender (23.3% vs. 33.2%) and freezing rate (20.9% vs. 35.7%). When comparing different methods of sperm selection for IVF, no differences were observed on cleavage rate except for the fact that the concentration of sperm from swim-up method (20.6%) was significantly lower than the one obtained from the washing method (78.7%). The recovery technique of sperm does not affect sperm quality and the method of fast freezing was shown to be the most effective for cryopreservation of alpaca sperm. 2023-06-05T17:14:33Z 2023-06-05T17:14:33Z 2019-02-13 info:eu-repo/semantics/article Mamani-Mango, G.; Moina Gonzales, M.; Ramos Hidalgo, M.; Mendoza Mallma, J.; Ruiz Bejar, J.; Rivas Palma, V.; & Mellisho Salas, E. (2019). Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis. Biopreservation and biobanking, 17(1), 39-45. doi: 10.1089/bio.2018.0021 1947-5543 https://hdl.handle.net/20.500.12955/2177 https://doi.org/10.1089/bio.2018.0021 eng Biopreservation and Biobanking info:eu-repo/semantics/restrictedAccess application/pdf Mary Ann Liebert, Inc. US Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Cryopreservation Alpaca Spermatozoa Epididymis https://purl.org/pe-repo/ocde/ford#4.02.01 Cryopreservation Alpaca Epididymis Criopreservación Alpacas Spermatozoa Espermatozoo Epididimo Mamani Mango, Guiulfo Moina Gonzales, Milagros Ramos Hidalgo, Martín Mendoza Mallma, José Ruiz Béjar, Jaime Rivas Palma, Victoria Esther Mellisho Salas, Edwin Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis |
| title | Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis |
| title_full | Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis |
| title_fullStr | Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis |
| title_full_unstemmed | Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis |
| title_short | Effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis |
| title_sort | effect of extender and freezing rate on quality parameters and in vitro fertilization capacity of alpaca spermatozoa recovered from cauda epididymis |
| topic | Cryopreservation Alpaca Spermatozoa Epididymis https://purl.org/pe-repo/ocde/ford#4.02.01 Cryopreservation Alpaca Epididymis Criopreservación Alpacas Spermatozoa Espermatozoo Epididimo |
| url | https://hdl.handle.net/20.500.12955/2177 https://doi.org/10.1089/bio.2018.0021 |
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