Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders
Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. Methods: Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each co...
| Autores principales: | , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
Wolters Kluwer Medknow Publications
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2172 https://doi.org/10.1111/j.1745-7262.2005.00021.x |
| _version_ | 1855028584469495808 |
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| author | Santiani, Alexei Huanca López, Wilfredo Sapana Valdivia, Rómulo Huanca Mamani, Teodosio Sepúlveda, Néstor Sánchez, Raúl |
| author_browse | Huanca López, Wilfredo Huanca Mamani, Teodosio Santiani, Alexei Sapana Valdivia, Rómulo Sepúlveda, Néstor Sánchez, Raúl |
| author_facet | Santiani, Alexei Huanca López, Wilfredo Sapana Valdivia, Rómulo Huanca Mamani, Teodosio Sepúlveda, Néstor Sánchez, Raúl |
| author_sort | Santiani, Alexei |
| collection | Repositorio INIA |
| description | Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. Methods: Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender I-G; 2) extender I-EG; 3) extender II-G; and 4) extender II-EG. Semen was diluted in a two-step process: for cooling to 5 °C (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results: When compared, the motility after thawing was higher (P < 0.05) in groups II-EG (20.0 % ± 6.7 %) and II-G (15.3 % ± 4.1 %) than that in groups I-G (4.0 % ± 1.1 %) and I-EG (1.0 % ± 1.4 %). Viable spermatozoa with intact acrosomes in groups II-EG (18.7 % ± 2.9 %) and II-G (12.7 % ± 5.9 %) were higher than that in groups I-G (5.7 % ± 1.5 %) and I-EG (4.0 % ± 1.0 %). Conclusion: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders. |
| format | info:eu-repo/semantics/article |
| id | INIA2172 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wolters Kluwer Medknow Publications |
| publisherStr | Wolters Kluwer Medknow Publications |
| record_format | dspace |
| spelling | INIA21722023-09-22T01:49:47Z Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders Santiani, Alexei Huanca López, Wilfredo Sapana Valdivia, Rómulo Huanca Mamani, Teodosio Sepúlveda, Néstor Sánchez, Raúl Cryopreservation Glycerol Ethylene glycol Extenders Alpaca spermatozoa https://purl.org/pe-repo/ocde/ford#4.02.01 Cryopreservation Criopreservación Ethylene glicol Etilenoglicol Alpacas Alpaca Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. Methods: Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender I-G; 2) extender I-EG; 3) extender II-G; and 4) extender II-EG. Semen was diluted in a two-step process: for cooling to 5 °C (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results: When compared, the motility after thawing was higher (P < 0.05) in groups II-EG (20.0 % ± 6.7 %) and II-G (15.3 % ± 4.1 %) than that in groups I-G (4.0 % ± 1.1 %) and I-EG (1.0 % ± 1.4 %). Viable spermatozoa with intact acrosomes in groups II-EG (18.7 % ± 2.9 %) and II-G (12.7 % ± 5.9 %) were higher than that in groups I-G (5.7 % ± 1.5 %) and I-EG (4.0 % ± 1.0 %). Conclusion: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders. 2023-06-05T17:10:22Z 2023-06-05T17:10:22Z 2005-09-01 info:eu-repo/semantics/article Santiani, A.; Huanca, W.; Sapana, R.; Huanca, T.; Sepúlveda, N.; & Sánchez, R. (2005). Effects on the quality of frozen‐thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders. Asian journal of Andrology, 7(3), 303-309. doi: 10.1111/j.1745-7262.2005.00021.x 1008-682X https://hdl.handle.net/20.500.12955/2172 https://doi.org/10.1111/j.1745-7262.2005.00021.x eng Asian Journal of Andrology info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf application/pdf Wolters Kluwer Medknow Publications IN Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Cryopreservation Glycerol Ethylene glycol Extenders Alpaca spermatozoa https://purl.org/pe-repo/ocde/ford#4.02.01 Cryopreservation Criopreservación Ethylene glicol Etilenoglicol Alpacas Alpaca Santiani, Alexei Huanca López, Wilfredo Sapana Valdivia, Rómulo Huanca Mamani, Teodosio Sepúlveda, Néstor Sánchez, Raúl Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders |
| title | Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders |
| title_full | Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders |
| title_fullStr | Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders |
| title_full_unstemmed | Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders |
| title_short | Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders |
| title_sort | effects on the quality of frozen thawed alpaca lama pacos semen using two different cryoprotectants and extenders |
| topic | Cryopreservation Glycerol Ethylene glycol Extenders Alpaca spermatozoa https://purl.org/pe-repo/ocde/ford#4.02.01 Cryopreservation Criopreservación Ethylene glicol Etilenoglicol Alpacas Alpaca |
| url | https://hdl.handle.net/20.500.12955/2172 https://doi.org/10.1111/j.1745-7262.2005.00021.x |
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