Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru
Eighty bacterial strains were isolated from root nodules of cowpea (Vigna unguiculata) plants agriculturally grown in Northern Peru. After isolation, fifty of them reinfected cowpea and eight showed better symbiotic performance than control plants treated with nitrate both under greenhouse and field...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:eu-repo/semantics/conferenceObject |
| Lenguaje: | Inglés |
| Publicado: |
Springer International Publishing
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2146 https://doi.org/10.1007/978-3-319-32528-6_17 |
| _version_ | 1855028605208231936 |
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| author | Valdez, Renzo Soriano, Bertha Prado, Gardenia Zavaleta, David Matsubara, Minoru Zúñiga Dávila, Doris Elizabeth Dion, Patricie Valladolid, Ángel Ruesta Campoverde, Nelson Asdrúbal Castellano Hinojosa, Antonio Correa Galeote, David Bedmar, Eulogio J. |
| author_browse | Bedmar, Eulogio J. Castellano Hinojosa, Antonio Correa Galeote, David Dion, Patricie Matsubara, Minoru Prado, Gardenia Ruesta Campoverde, Nelson Asdrúbal Soriano, Bertha Valdez, Renzo Valladolid, Ángel Zavaleta, David Zúñiga Dávila, Doris Elizabeth |
| author_facet | Valdez, Renzo Soriano, Bertha Prado, Gardenia Zavaleta, David Matsubara, Minoru Zúñiga Dávila, Doris Elizabeth Dion, Patricie Valladolid, Ángel Ruesta Campoverde, Nelson Asdrúbal Castellano Hinojosa, Antonio Correa Galeote, David Bedmar, Eulogio J. |
| author_sort | Valdez, Renzo |
| collection | Repositorio INIA |
| description | Eighty bacterial strains were isolated from root nodules of cowpea (Vigna unguiculata) plants agriculturally grown in Northern Peru. After isolation, fifty of them reinfected cowpea and eight showed better symbiotic performance than control plants treated with nitrate both under greenhouse and field. Repetitive extragenic palindromic polymerase chain reaction (REP-PCR) clustered the strains into 3 REP-PCR groups. The partial sequence of the 16S rRNA gene from a representative strain of each REP-PCR pattern showed that the strains were closely related to members of genus Bradyrhizobium of the Alphaproteobacteria, but affiliation at the species level was not clear. Pairwise alignments among globally aligned sequences of the 16S rRNA genes, the sequencing of the housekeeping genes atpD, glnII, and recA, and their concatenated phylogenetic analysis showed that strains Rc-391-01 and Rc-458-01 grouped with B. yuanmingense B071T and that strain Rc-352-01 clustered with Bradyrhizobium liaoningense 2281T. Examination of the three representative strains to behave as plant growth-promoting rhizobacteria (PGPRs) showed that none of them solubilized calcium triphosphate and that they all three produced siderophores and IAA-related compounds and had ACC-deaminase activity. |
| format | info:eu-repo/semantics/conferenceObject |
| id | INIA2146 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer International Publishing |
| publisherStr | Springer International Publishing |
| record_format | dspace |
| spelling | INIA21462025-05-25T23:56:50Z Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru Valdez, Renzo Soriano, Bertha Prado, Gardenia Zavaleta, David Matsubara, Minoru Zúñiga Dávila, Doris Elizabeth Dion, Patricie Valladolid, Ángel Ruesta Campoverde, Nelson Asdrúbal Castellano Hinojosa, Antonio Correa Galeote, David Bedmar, Eulogio J. Bradyrhizobium Cowpea Symbiosis 16S rRNA Housekeeping genes PGPRs https://purl.org/pe-repo/ocde/ford#4.01.06 Bradyrhizobium Caupí Cowpeas Eighty bacterial strains were isolated from root nodules of cowpea (Vigna unguiculata) plants agriculturally grown in Northern Peru. After isolation, fifty of them reinfected cowpea and eight showed better symbiotic performance than control plants treated with nitrate both under greenhouse and field. Repetitive extragenic palindromic polymerase chain reaction (REP-PCR) clustered the strains into 3 REP-PCR groups. The partial sequence of the 16S rRNA gene from a representative strain of each REP-PCR pattern showed that the strains were closely related to members of genus Bradyrhizobium of the Alphaproteobacteria, but affiliation at the species level was not clear. Pairwise alignments among globally aligned sequences of the 16S rRNA genes, the sequencing of the housekeeping genes atpD, glnII, and recA, and their concatenated phylogenetic analysis showed that strains Rc-391-01 and Rc-458-01 grouped with B. yuanmingense B071T and that strain Rc-352-01 clustered with Bradyrhizobium liaoningense 2281T. Examination of the three representative strains to behave as plant growth-promoting rhizobacteria (PGPRs) showed that none of them solubilized calcium triphosphate and that they all three produced siderophores and IAA-related compounds and had ACC-deaminase activity. 2023-04-27T00:49:47Z 2023-04-27T00:49:47Z 2016-06-03 info:eu-repo/semantics/conferenceObject Valdez, R., Soriano, B., Prado, G., Zavaleta, D., Matsubara, M., Zúñiga, D., ... & Bedmar, E. (2016). Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru. In Biological nitrogen fixation and beneficial plant-microbe interaction, 195-212. doi: 10.1007/978-3-319-32528-6_17 978-3-319-32528-6 https://hdl.handle.net/20.500.12955/2146 https://doi.org/10.1007/978-3-319-32528-6_17 eng urn:isbn:978-3-319-32528-6 Biological Nitrogen Fixation and Beneficial Plant-Microbe Interaction info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf Springer International Publishing CH Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Bradyrhizobium Cowpea Symbiosis 16S rRNA Housekeeping genes PGPRs https://purl.org/pe-repo/ocde/ford#4.01.06 Bradyrhizobium Caupí Cowpeas Valdez, Renzo Soriano, Bertha Prado, Gardenia Zavaleta, David Matsubara, Minoru Zúñiga Dávila, Doris Elizabeth Dion, Patricie Valladolid, Ángel Ruesta Campoverde, Nelson Asdrúbal Castellano Hinojosa, Antonio Correa Galeote, David Bedmar, Eulogio J. Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru |
| title | Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru |
| title_full | Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru |
| title_fullStr | Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru |
| title_full_unstemmed | Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru |
| title_short | Symbiotic and agronomic characterization of Bradyrhizobial strains nodulating cowpea in Northern Peru |
| title_sort | symbiotic and agronomic characterization of bradyrhizobial strains nodulating cowpea in northern peru |
| topic | Bradyrhizobium Cowpea Symbiosis 16S rRNA Housekeeping genes PGPRs https://purl.org/pe-repo/ocde/ford#4.01.06 Bradyrhizobium Caupí Cowpeas |
| url | https://hdl.handle.net/20.500.12955/2146 https://doi.org/10.1007/978-3-319-32528-6_17 |
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