Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro
This study evaluated alpaca oocytes that developed to metaphase II (MII), using different concentrations of follicle stimulating hormone (FSH) with and without equine chorionic gonadotropin (eCG) during in vitro maturation. Oocytes were obtained from ovaries of slaughtered alpacas. Oocytes were matu...
| Autores principales: | , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
Unique Scientific Publishers
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2133 https://doi.org/10.47278/journal.ijvs/2021.050 |
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| author | Quispe Gutiérrez, US Olivera Marocho, LV Ccopa Ccallata, Jhunior Pahuara Farfan, Lariza Evelyn Barragán Condori, M Berndtson, JL |
| author_browse | Barragán Condori, M Berndtson, JL Ccopa Ccallata, Jhunior Olivera Marocho, LV Pahuara Farfan, Lariza Evelyn Quispe Gutiérrez, US |
| author_facet | Quispe Gutiérrez, US Olivera Marocho, LV Ccopa Ccallata, Jhunior Pahuara Farfan, Lariza Evelyn Barragán Condori, M Berndtson, JL |
| author_sort | Quispe Gutiérrez, US |
| collection | Repositorio INIA |
| description | This study evaluated alpaca oocytes that developed to metaphase II (MII), using different concentrations of follicle stimulating hormone (FSH) with and without equine chorionic gonadotropin (eCG) during in vitro maturation. Oocytes were obtained from ovaries of slaughtered alpacas. Oocytes were matured in vitro for 36h in TCM-99 and supplemented in groups with different doses of FSH: 0.5, 0.25, 0μg.mL-1 and eCG: 15, 5, 0IU.mL-1. Oocytes were stained with 2% lacmoid, and examined for their nuclear status. The parameter for comparisons between groups was the percentage of oocytes in MII. There was an interaction between FSH and eCG (P<0.05). A higher percentage of oocytes in MII were obtained when using 0.5μg.mL-1 of FSH with any concentration of 15, 5 or 0IU.mL-1 of eCG [58.4±1.94% (n=78); 59.5±1.94% (n=85); 54.3±0.56% (n=81); P<0.05]. A similar percentage was also found using 0.25μg.mL-1 of FSH plus 15IU.mL-1 of eCG [52.3±1.66% (n=86); P>0.05] followed by 0.25μg.mL-1 of FSH with 5 or 0IU.mL-1 of eCG [47.3±1.94% (n=82); 45.3±1.94% (n=86); P<0.05]. The lowest maturation percentages were found using any concentration of eCG without FSH [8.2±0.73% (n=84); 8.0±0.66% (n=74); 11.4±1.23% (n=71); P<0.05]. In conclusion, the addition of eCG to the maturation medium would reduce the amount of FSH required in the in vitro maturation of alpaca oocytes. |
| format | info:eu-repo/semantics/article |
| id | INIA2133 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Unique Scientific Publishers |
| publisherStr | Unique Scientific Publishers |
| record_format | dspace |
| spelling | INIA21332025-05-25T23:55:51Z Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro Quispe Gutiérrez, US Olivera Marocho, LV Ccopa Ccallata, Jhunior Pahuara Farfan, Lariza Evelyn Barragán Condori, M Berndtson, JL Camelid Equine chorionic gonadotropin Follicle stimulating hormone Metaphase II https://purl.org/pe-repo/ocde/ford#4.02.01 Camelidae PMSG FSH Alpacas This study evaluated alpaca oocytes that developed to metaphase II (MII), using different concentrations of follicle stimulating hormone (FSH) with and without equine chorionic gonadotropin (eCG) during in vitro maturation. Oocytes were obtained from ovaries of slaughtered alpacas. Oocytes were matured in vitro for 36h in TCM-99 and supplemented in groups with different doses of FSH: 0.5, 0.25, 0μg.mL-1 and eCG: 15, 5, 0IU.mL-1. Oocytes were stained with 2% lacmoid, and examined for their nuclear status. The parameter for comparisons between groups was the percentage of oocytes in MII. There was an interaction between FSH and eCG (P<0.05). A higher percentage of oocytes in MII were obtained when using 0.5μg.mL-1 of FSH with any concentration of 15, 5 or 0IU.mL-1 of eCG [58.4±1.94% (n=78); 59.5±1.94% (n=85); 54.3±0.56% (n=81); P<0.05]. A similar percentage was also found using 0.25μg.mL-1 of FSH plus 15IU.mL-1 of eCG [52.3±1.66% (n=86); P>0.05] followed by 0.25μg.mL-1 of FSH with 5 or 0IU.mL-1 of eCG [47.3±1.94% (n=82); 45.3±1.94% (n=86); P<0.05]. The lowest maturation percentages were found using any concentration of eCG without FSH [8.2±0.73% (n=84); 8.0±0.66% (n=74); 11.4±1.23% (n=71); P<0.05]. In conclusion, the addition of eCG to the maturation medium would reduce the amount of FSH required in the in vitro maturation of alpaca oocytes. 2023-04-10T16:10:44Z 2023-04-10T16:10:44Z 2021-07-15 info:eu-repo/semantics/article Quispe Gutiérrez, U. S., Olivera Marocho, L. V., Ccopa Ccallata, J., Pahuara Farfán, L. E., Barragán Condori, M., & Berndtson, J. L. (2021). Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro. International Journal of Veterinary Science, 10(3), 156-161. doi: 10.47278/journal.ijvs/2021.050 2304-3075 https://hdl.handle.net/20.500.12955/2133 https://doi.org/10.47278/journal.ijvs/2021.050 eng urn:issn:2304-3075 International Journal of Veterinary Science info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/4.0/ application/pdf application/pdf Unique Scientific Publishers PK Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Camelid Equine chorionic gonadotropin Follicle stimulating hormone Metaphase II https://purl.org/pe-repo/ocde/ford#4.02.01 Camelidae PMSG FSH Alpacas Quispe Gutiérrez, US Olivera Marocho, LV Ccopa Ccallata, Jhunior Pahuara Farfan, Lariza Evelyn Barragán Condori, M Berndtson, JL Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro |
| title | Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro |
| title_full | Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro |
| title_fullStr | Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro |
| title_full_unstemmed | Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro |
| title_short | Effect of FSH and eCG on alpaca (Vicugna pacos) oocyte maturation in vitro |
| title_sort | effect of fsh and ecg on alpaca vicugna pacos oocyte maturation in vitro |
| topic | Camelid Equine chorionic gonadotropin Follicle stimulating hormone Metaphase II https://purl.org/pe-repo/ocde/ford#4.02.01 Camelidae PMSG FSH Alpacas |
| url | https://hdl.handle.net/20.500.12955/2133 https://doi.org/10.47278/journal.ijvs/2021.050 |
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