Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster
Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despit...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | https://hdl.handle.net/20.500.12955/2090 https://doi.org/10.3390/vaccines11010111 |
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| author | Maia Gonçalves, Ana Alice Ottino, Jennifer Costa Leite, Jaqueline Aparecida Resende, Lucilene Melo Júnior, Otoni Alves Silveira, Patrícia Santos Cardoso, Mariana Toshio Fujiwara, Ricardo Lacerda Bueno, Lilian Lima Santos, Renato Furtado de Carvalho, Tatiane Martins Garcia, Giani de Oliveira Paes, Paulo Ricardo Sobreira Galdino, Alexsandro Chávez Fumagalli, Miguel Angel Martins Melo, Marília Silveira Lemos, Denise Martins Filho, Olindo Assis Ornelas Dutra, Walderez Furtado Mosqueira, Vanessa Carla Cordeiro Giunchetti, Rodolfo |
| author_browse | Aparecida Resende, Lucilene Chávez Fumagalli, Miguel Angel Cordeiro Giunchetti, Rodolfo Costa Leite, Jaqueline Furtado Mosqueira, Vanessa Carla Furtado de Carvalho, Tatiane Lacerda Bueno, Lilian Lima Santos, Renato Maia Gonçalves, Ana Alice Martins Filho, Olindo Assis Martins Garcia, Giani Martins Melo, Marília Melo Júnior, Otoni Alves Ornelas Dutra, Walderez Ottino, Jennifer Santos Cardoso, Mariana Silveira Lemos, Denise Silveira, Patrícia Sobreira Galdino, Alexsandro Toshio Fujiwara, Ricardo de Oliveira Paes, Paulo Ricardo |
| author_facet | Maia Gonçalves, Ana Alice Ottino, Jennifer Costa Leite, Jaqueline Aparecida Resende, Lucilene Melo Júnior, Otoni Alves Silveira, Patrícia Santos Cardoso, Mariana Toshio Fujiwara, Ricardo Lacerda Bueno, Lilian Lima Santos, Renato Furtado de Carvalho, Tatiane Martins Garcia, Giani de Oliveira Paes, Paulo Ricardo Sobreira Galdino, Alexsandro Chávez Fumagalli, Miguel Angel Martins Melo, Marília Silveira Lemos, Denise Martins Filho, Olindo Assis Ornelas Dutra, Walderez Furtado Mosqueira, Vanessa Carla Cordeiro Giunchetti, Rodolfo |
| author_sort | Maia Gonçalves, Ana Alice |
| collection | Repositorio INIA |
| description | Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading Leishmania amazonensis antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-Leishmania IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups showed an intense reduction in liver and spleen parasitic load by qPCR. The LAPSmP and LAPSmG vaccines showed exceptional results, indicating that they may be promising candidates as a VL vaccine. |
| format | Artículo |
| id | INIA2090 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INIA20902023-05-02T23:32:55Z Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster Maia Gonçalves, Ana Alice Ottino, Jennifer Costa Leite, Jaqueline Aparecida Resende, Lucilene Melo Júnior, Otoni Alves Silveira, Patrícia Santos Cardoso, Mariana Toshio Fujiwara, Ricardo Lacerda Bueno, Lilian Lima Santos, Renato Furtado de Carvalho, Tatiane Martins Garcia, Giani de Oliveira Paes, Paulo Ricardo Sobreira Galdino, Alexsandro Chávez Fumagalli, Miguel Angel Martins Melo, Marília Silveira Lemos, Denise Martins Filho, Olindo Assis Ornelas Dutra, Walderez Furtado Mosqueira, Vanessa Carla Cordeiro Giunchetti, Rodolfo Visceral leishmaniasis Nanotechnology Vaccine https://purl.org/pe-repo/ocde/ford#4.03.01 Leishmaniasis Nanotechnology Vaccines Leishmania Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading Leishmania amazonensis antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-Leishmania IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups showed an intense reduction in liver and spleen parasitic load by qPCR. The LAPSmP and LAPSmG vaccines showed exceptional results, indicating that they may be promising candidates as a VL vaccine. 2023-02-23T15:26:20Z 2023-02-23T15:26:20Z 2023-01-02 info:eu-repo/semantics/article González, M.; Gonçalves, A.; Ottino, J.; Leite, J.; Resende, L.; Melo, O.; Silveira, P.; Cardoso, M.; Fujiwara, R.; Bueno, L.; Santos, R.; Carvalho, T.; Garcia, G.; Paes.; Galdino, A.; Chávez, M.; Melo, M.; Silveira, D.; Martins, O.; … Giunchetti, R. (2023). Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster. Vaccines, 11(1), 111. doi: 10.3390/vaccines11010111 2076-393X https://hdl.handle.net/20.500.12955/2090 https://doi.org/10.3390/vaccines11010111 eng urn:issn:2076-393X Vaccines info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf application/pdf MDPI CH Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Visceral leishmaniasis Nanotechnology Vaccine https://purl.org/pe-repo/ocde/ford#4.03.01 Leishmaniasis Nanotechnology Vaccines Leishmania Maia Gonçalves, Ana Alice Ottino, Jennifer Costa Leite, Jaqueline Aparecida Resende, Lucilene Melo Júnior, Otoni Alves Silveira, Patrícia Santos Cardoso, Mariana Toshio Fujiwara, Ricardo Lacerda Bueno, Lilian Lima Santos, Renato Furtado de Carvalho, Tatiane Martins Garcia, Giani de Oliveira Paes, Paulo Ricardo Sobreira Galdino, Alexsandro Chávez Fumagalli, Miguel Angel Martins Melo, Marília Silveira Lemos, Denise Martins Filho, Olindo Assis Ornelas Dutra, Walderez Furtado Mosqueira, Vanessa Carla Cordeiro Giunchetti, Rodolfo Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster |
| title | Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster |
| title_full | Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster |
| title_fullStr | Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster |
| title_full_unstemmed | Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster |
| title_short | Vaccination with formulation of nanoparticles loaded with Leishmania amazonensis antigens confers protection against experimental visceral Leishmaniasis in hamster |
| title_sort | vaccination with formulation of nanoparticles loaded with leishmania amazonensis antigens confers protection against experimental visceral leishmaniasis in hamster |
| topic | Visceral leishmaniasis Nanotechnology Vaccine https://purl.org/pe-repo/ocde/ford#4.03.01 Leishmaniasis Nanotechnology Vaccines Leishmania |
| url | https://hdl.handle.net/20.500.12955/2090 https://doi.org/10.3390/vaccines11010111 |
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