Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction
Microbial interactions between filamentous fungi and yeast are still not fully understood. To evaluate a potential antifungal activity of a filamentous fungus while highlighting metabolomic changes, co-cultures between an endophytic strain of Cophinforma mamane (CM) and Candida albicans (CA) were pe...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2087 https://doi.org/10.1007/s13659-022-00365-w |
| _version_ | 1855490253882654720 |
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| author | Triastuti, Asih Vansteelandt, Marieke Barakat, Fatima Amasifuen Guerra, Carlos Alberto Jargeat, Patricia Haddad, Mohamed |
| author_browse | Amasifuen Guerra, Carlos Alberto Barakat, Fatima Haddad, Mohamed Jargeat, Patricia Triastuti, Asih Vansteelandt, Marieke |
| author_facet | Triastuti, Asih Vansteelandt, Marieke Barakat, Fatima Amasifuen Guerra, Carlos Alberto Jargeat, Patricia Haddad, Mohamed |
| author_sort | Triastuti, Asih |
| collection | Repositorio INIA |
| description | Microbial interactions between filamentous fungi and yeast are still not fully understood. To evaluate a potential antifungal activity of a filamentous fungus while highlighting metabolomic changes, co-cultures between an endophytic strain of Cophinforma mamane (CM) and Candida albicans (CA) were performed. The liquid cultures were incubated under static conditions and metabolite alterations during the course were investigated by ultra-performance liquid chromatography–tandem mass spectrophotometry (UPLC–MS/MS). Results were analyzed using MS-DIAL, MS-FINDER, METLIN, Xcalibur, SciFinder, and MetaboAnalyst metabolomics platforms. The metabolites associated with catabolic processes, including the metabolism of branched-chain amino acids, carnitine, and phospholipids were upregulated both in the mono and co-cultures, indicating fungal adaptability to environmental stress. Several metabolites, including C20 sphinganine 1-phosphate, myo-inositol, farnesol, gamma-undecalactone, folinic acid, palmitoleic acid, and MG (12:/0:0/0:0) were not produced by CA during co-culture with CM, demonstrating the antifungal mechanism of CM. Our results highlight the crucial roles of metabolomics studies to provide essential information regarding the antifungal mechanism of C. mamane against C. albicans, especially when the lost/undetected metabolites are involved in fungal survival and pathogenicity. |
| format | Artículo |
| id | INIA2087 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INIA20872023-05-02T21:51:35Z Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction Triastuti, Asih Vansteelandt, Marieke Barakat, Fatima Amasifuen Guerra, Carlos Alberto Jargeat, Patricia Haddad, Mohamed Metabolomics Fungal co-culture Anti-fungal Virulence https://purl.org/pe-repo/ocde/ford#1.06.05 Metabolomics Virulence Candida albicans Fungicides Microbial interactions between filamentous fungi and yeast are still not fully understood. To evaluate a potential antifungal activity of a filamentous fungus while highlighting metabolomic changes, co-cultures between an endophytic strain of Cophinforma mamane (CM) and Candida albicans (CA) were performed. The liquid cultures were incubated under static conditions and metabolite alterations during the course were investigated by ultra-performance liquid chromatography–tandem mass spectrophotometry (UPLC–MS/MS). Results were analyzed using MS-DIAL, MS-FINDER, METLIN, Xcalibur, SciFinder, and MetaboAnalyst metabolomics platforms. The metabolites associated with catabolic processes, including the metabolism of branched-chain amino acids, carnitine, and phospholipids were upregulated both in the mono and co-cultures, indicating fungal adaptability to environmental stress. Several metabolites, including C20 sphinganine 1-phosphate, myo-inositol, farnesol, gamma-undecalactone, folinic acid, palmitoleic acid, and MG (12:/0:0/0:0) were not produced by CA during co-culture with CM, demonstrating the antifungal mechanism of CM. Our results highlight the crucial roles of metabolomics studies to provide essential information regarding the antifungal mechanism of C. mamane against C. albicans, especially when the lost/undetected metabolites are involved in fungal survival and pathogenicity. 2023-02-23T15:25:30Z 2023-02-23T15:25:30Z 2023-01-03 info:eu-repo/semantics/article Triastuti, A., Vansteelandt, M., Barakat, F., Amasifuen, C., Jargeat, P., & Haddad, M. (2023). Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction. Natural Products and Bioprospecting, 13(1), 1. doi: 10.1007/s13659-022-00365-w 2192-2209 https://hdl.handle.net/20.500.12955/2087 https://doi.org/10.1007/s13659-022-00365-w eng urn:issn:2192-2209 Natural products and bioprospecting info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Springer DE Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Metabolomics Fungal co-culture Anti-fungal Virulence https://purl.org/pe-repo/ocde/ford#1.06.05 Metabolomics Virulence Candida albicans Fungicides Triastuti, Asih Vansteelandt, Marieke Barakat, Fatima Amasifuen Guerra, Carlos Alberto Jargeat, Patricia Haddad, Mohamed Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction |
| title | Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction |
| title_full | Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction |
| title_fullStr | Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction |
| title_full_unstemmed | Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction |
| title_short | Untargeted metabolomics to evaluate antifungal mechanism: a study of Cophinforma mamane and Candida albicans interaction |
| title_sort | untargeted metabolomics to evaluate antifungal mechanism a study of cophinforma mamane and candida albicans interaction |
| topic | Metabolomics Fungal co-culture Anti-fungal Virulence https://purl.org/pe-repo/ocde/ford#1.06.05 Metabolomics Virulence Candida albicans Fungicides |
| url | https://hdl.handle.net/20.500.12955/2087 https://doi.org/10.1007/s13659-022-00365-w |
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