Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids
10 páginas
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
El Sevier
2022
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/2005 https://doi.org/10.1016/j.funbio.2022.02.005 |
| _version_ | 1855490312380612608 |
|---|---|
| author | Pacheco Tapia, R. Vásquez Ocmín, Pedro Duthen, S. Ortíz, S. Jargeat, P. Amasifuen Guerra, Carlos Alberto Haddad, Mohamed Vansteelandt, Marieke |
| author_browse | Amasifuen Guerra, Carlos Alberto Duthen, S. Haddad, Mohamed Jargeat, P. Ortíz, S. Pacheco Tapia, R. Vansteelandt, Marieke Vásquez Ocmín, Pedro |
| author_facet | Pacheco Tapia, R. Vásquez Ocmín, Pedro Duthen, S. Ortíz, S. Jargeat, P. Amasifuen Guerra, Carlos Alberto Haddad, Mohamed Vansteelandt, Marieke |
| author_sort | Pacheco Tapia, R. |
| collection | Repositorio INIA |
| description | 10 páginas |
| format | Artículo |
| id | INIA2005 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | El Sevier |
| publisherStr | El Sevier |
| record_format | dspace |
| spelling | INIA20052023-12-19T16:44:14Z Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids Pacheco Tapia, R. Vásquez Ocmín, Pedro Duthen, S. Ortíz, S. Jargeat, P. Amasifuen Guerra, Carlos Alberto Haddad, Mohamed Vansteelandt, Marieke Cophinforma mamane Diketopiperazines Endophytic fungi Epigenetic modifiers Metabolomics https://purl.org/pe-repo/ocde/ford#4.04.00 10 páginas Endophytic fungi are capable of producing a great diversity of bioactive metabolites. However, the presence of silent and lowly expressed genes represents a main challenge for the discovery of novel secondary metabolites with different potential uses. Epigenetic modifiers have shown to perturb the production of fungal metabolites through the induction of silent biosynthetic pathways leading to an enhanced chemical diversity. Moreover, the addition of bioprecursors to the culture medium has been described as a useful strategy to induce specific biosynthetic pathways. The aim of this study was to assess the effects of different chemical modulators on the metabolic profiles of an endophytic fungal strain of Cophinforma mamane (Botryosphaeriaceae), known to produce 3 thiodiketopiperazine (TDKP) alkaloids (botryosulfuranols A-C), previously isolated and characterized by our team. Four epigenetic modifiers, 5-azacytidine (AZA), sodium butyrate (SB), nicotinamide (NIC), homoserine lactone (HSL) as well as 2 amino acids, L-phenylalanine and L-tryptophan, as bioprecursors of TDKPs, were used. The metabolic profiles were analysed by UHPLC-HRMS/MS under an untargeted metabolomics approach. Our results show that the addition of the two amino acids in C. mamane culture and the treatment with AZA significantly reduced the production of the TDKPs botryosulfuranols A, B and C. Interestingly, the treatment with HSL significantly induced the production of different classes of diketopiperazines (DKPs). The treatment with AZA resulted as the most effective epigenetic modifier for the alteration of the secondary metabolite profile of C. mamane by promoting the expression of cryptic genes. 2022-11-30T17:01:16Z 2022-11-30T17:01:16Z 2022-05 info:eu-repo/semantics/article Pacheco, R.; Vásquez, P.; Duthen, S. et al. (2022). Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids. Fungal Biology, 126(5), 385-394. doi: 10.1016/j.funbio.2022.02.005 https://hdl.handle.net/20.500.12955/2005 https://doi.org/10.1016/j.funbio.2022.02.005 eng Fungal Biology https://doi.org/10.1016/j.funbio.2022.02.005 info:eu-repo/semantics/restrictedAccess Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ application/pdf application/pdf El Sevier CH Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Cophinforma mamane Diketopiperazines Endophytic fungi Epigenetic modifiers Metabolomics https://purl.org/pe-repo/ocde/ford#4.04.00 Pacheco Tapia, R. Vásquez Ocmín, Pedro Duthen, S. Ortíz, S. Jargeat, P. Amasifuen Guerra, Carlos Alberto Haddad, Mohamed Vansteelandt, Marieke Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids |
| title | Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids |
| title_full | Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids |
| title_fullStr | Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids |
| title_full_unstemmed | Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids |
| title_short | Chemical modulation of the metabolism of an endophytic fungal strain of Cophinforma mamane using epigenetic modifiers and amino-acids |
| title_sort | chemical modulation of the metabolism of an endophytic fungal strain of cophinforma mamane using epigenetic modifiers and amino acids |
| topic | Cophinforma mamane Diketopiperazines Endophytic fungi Epigenetic modifiers Metabolomics https://purl.org/pe-repo/ocde/ford#4.04.00 |
| url | https://hdl.handle.net/20.500.12955/2005 https://doi.org/10.1016/j.funbio.2022.02.005 |
| work_keys_str_mv | AT pachecotapiar chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids AT vasquezocminpedro chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids AT duthens chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids AT ortizs chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids AT jargeatp chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids AT amasifuenguerracarlosalberto chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids AT haddadmohamed chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids AT vansteelandtmarieke chemicalmodulationofthemetabolismofanendophyticfungalstrainofcophinformamamaneusingepigeneticmodifiersandaminoacids |