Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene
9 páginas
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
El Sevier
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/1987 http://doi.org/10.1016/j.virusres.2017.03.019 |
| _version_ | 1855490448999579648 |
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| author | Lozano, Ivan Leiva, Ana M. Jimenez, Jenyfer Fernández Huaytalla, Elizabeth Carvajal Yepes, Monica Cuervo, Maritza Cuellar, Wilmer J. |
| author_browse | Carvajal Yepes, Monica Cuellar, Wilmer J. Cuervo, Maritza Fernández Huaytalla, Elizabeth Jimenez, Jenyfer Leiva, Ana M. Lozano, Ivan |
| author_facet | Lozano, Ivan Leiva, Ana M. Jimenez, Jenyfer Fernández Huaytalla, Elizabeth Carvajal Yepes, Monica Cuervo, Maritza Cuellar, Wilmer J. |
| author_sort | Lozano, Ivan |
| collection | Repositorio INIA |
| description | 9 páginas |
| format | Artículo |
| id | INIA1987 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | El Sevier |
| publisherStr | El Sevier |
| record_format | dspace |
| spelling | INIA19872024-06-19T06:34:48Z Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene Lozano, Ivan Leiva, Ana M. Jimenez, Jenyfer Fernández Huaytalla, Elizabeth Carvajal Yepes, Monica Cuervo, Maritza Cuellar, Wilmer J. Alphaflexiviridae Potexvirus Triple gene block Manihot esculenta Virus diagnostics. https://purl.org/pe-repo/ocde/ford#4.04.00 9 páginas Several potexviruses (Family Alphaflexiviridae) have been reported infecting cassava (Manihot esculenta Crantz) in the Americas. They were isolated from severely diseased plants during the last 30–40 years and include: Cassava common mosaic virus (CsCMV), Cassava Caribbean mosaic virus (CsCaMV), Cassava Colombian symptomless virus (CsCSV) and Cassava virus X (CsVX). However, their definitive classification as distinct species remains unresolved for several reasons, including the lack of sequence data and unavailability of samples from original isolates. This complicates disease diagnostics, cassava germplasm exchange certification, evaluation of virus cleaning protocols and epidemiological studies. Furthermore, a recently detected novel alphaflexivirus, indicates that cassava-infecting potexviruses may be more diverse. To solve the identity of these viruses, we started indexing samples from different parts of Colombia using different sets of PCR primers, antisera available and inoculation to indicator plants. Results show that there are three major phylogenetic groups of potexviruses infecting cassava, and they correspond to CsCMV, CsVX and the newly identified Cassava new alphaflexivirus (CsNAV). Bioassays and sequence analysis established that isolates of CsNAV and CsVX cause latent infections in different cassava landraces, they are not efficiently transmitted to the indicator plant Nicotiana benthamiana and they lack the gene 3 of the conserved potexviral ‘triple gene block’ (TGB). In contrast, all isolates of CsCMV (which have a characteristic potexvirus genome arrangement) caused Cassava Common Mosaic Disease (CCMD) in single infections and were efficiently transmitted to N. benthamiana. Although phylogenetic analysis of the replicase sequence placed CsNAV and CsVX as members of the Potexvirus genus, their distinct genome arrangement and biological characteristics suggest they can be considered as members of a separate taxonomic group. 2022-11-30T16:56:57Z 2022-11-30T16:56:57Z 2017-05-30 info:eu-repo/semantics/article Lozano, I.; Leiva, A.; Jimenez, J. et al. (2017). Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene. Virus Research, 241(15), 53-61. doi: 10.1016/j.virusres.2017.03.019 https://hdl.handle.net/20.500.12955/1987 http://doi.org/10.1016/j.virusres.2017.03.019 eng Virus Research https://pgc-snia.inia.gob.pe:8443/jspui/cris/project/pj00213 http://doi.org/10.1016/j.virusres.2017.03.019 Identificación de los virus que afectan al cultivo de yuca (Manihot esculenta crantz) en el banco de germoplasma del INIA y en campos de agricultores de las regiones de Loreto, Ucayali y Lima info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by/4.0/ application/pdf El Sevier CH Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Alphaflexiviridae Potexvirus Triple gene block Manihot esculenta Virus diagnostics. https://purl.org/pe-repo/ocde/ford#4.04.00 Lozano, Ivan Leiva, Ana M. Jimenez, Jenyfer Fernández Huaytalla, Elizabeth Carvajal Yepes, Monica Cuervo, Maritza Cuellar, Wilmer J. Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene |
| title | Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene |
| title_full | Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene |
| title_fullStr | Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene |
| title_full_unstemmed | Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene |
| title_short | Resolution of cassava-infecting alphaflexiviruses: Molecular and biological characterization of a novel group of potexviruses lacking the TGB3 gene |
| title_sort | resolution of cassava infecting alphaflexiviruses molecular and biological characterization of a novel group of potexviruses lacking the tgb3 gene |
| topic | Alphaflexiviridae Potexvirus Triple gene block Manihot esculenta Virus diagnostics. https://purl.org/pe-repo/ocde/ford#4.04.00 |
| url | https://hdl.handle.net/20.500.12955/1987 http://doi.org/10.1016/j.virusres.2017.03.019 |
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