Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
The alpaca is of greatest economic importance in the Peruvian High Andes. This study aimed to determine the genetic diversity of three Peruvian alpaca farms, as well as, to validate a microsatellite markers panel for paternity testing. In this study, 247 samples of Huacaya alpacas were taken from th...
| Main Authors: | , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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El Sevier
2022
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| Online Access: | https://hdl.handle.net/20.500.12955/1951 https://doi.org/10.1016/j.smallrumres.2020.106246 |
| _version_ | 1855028437830336512 |
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| author | Morón, J. A. Veli Rivera, Eudosio Amancio Membrillo, A. Paredes, M. M. Gutiérrez Reynoso, Gustavo Augusto |
| author_browse | Gutiérrez Reynoso, Gustavo Augusto Membrillo, A. Morón, J. A. Paredes, M. M. Veli Rivera, Eudosio Amancio |
| author_facet | Morón, J. A. Veli Rivera, Eudosio Amancio Membrillo, A. Paredes, M. M. Gutiérrez Reynoso, Gustavo Augusto |
| author_sort | Morón, J. A. |
| collection | Repositorio INIA |
| description | The alpaca is of greatest economic importance in the Peruvian High Andes. This study aimed to determine the genetic diversity of three Peruvian alpaca farms, as well as, to validate a microsatellite markers panel for paternity testing. In this study, 247 samples of Huacaya alpacas were taken from three different localities (Sanjo, San Pedro de Raco and Cachipampa) from Pasco Region in Peru. DNA was obtained from hair follicles and genotyped for 15 microsatellites markers in multiplex electrophoresis runs.A total of 225 alleles were detected across the 15 loci investigated. The polymorphism information content considering all loci was 0.82, which indicated that the microsatellite panel was very polymorphic and highly informative. The estimated diversity parameter showed that farms have high levels of genetic diversity (HE = 0.826), and revealed the existence of genetic differentiation among the farms (FST = 2.8 %). The highest inbreeding coefficient was in the Sanjo farm (FIS = 0.303). The results of the parentage testing indicated that all loci showed values greater than 70 % probability of discrimination. However, the highest values found were 94 % (YWLL08) and 90 % (YWLL36). The average of the probability of exclusion obtained was 0.999994 if the genotype for one alleged parents is known, and 0.99999 if the genotypes for both alleged parents are known. The results obtained show that there is a high genetic diversity and validate the panel of microsatellite markers, that would help to improve the identification system and genealogical data collection. |
| format | info:eu-repo/semantics/article |
| id | INIA1951 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | El Sevier |
| publisherStr | El Sevier |
| record_format | dspace |
| spelling | INIA19512023-12-19T16:40:20Z Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms Morón, J. A. Veli Rivera, Eudosio Amancio Membrillo, A. Paredes, M. M. Gutiérrez Reynoso, Gustavo Augusto Microsatellite Alpaca Genetic diversity Parentage testing https://purl.org/pe-repo/ocde/ford#4.04.02 The alpaca is of greatest economic importance in the Peruvian High Andes. This study aimed to determine the genetic diversity of three Peruvian alpaca farms, as well as, to validate a microsatellite markers panel for paternity testing. In this study, 247 samples of Huacaya alpacas were taken from three different localities (Sanjo, San Pedro de Raco and Cachipampa) from Pasco Region in Peru. DNA was obtained from hair follicles and genotyped for 15 microsatellites markers in multiplex electrophoresis runs.A total of 225 alleles were detected across the 15 loci investigated. The polymorphism information content considering all loci was 0.82, which indicated that the microsatellite panel was very polymorphic and highly informative. The estimated diversity parameter showed that farms have high levels of genetic diversity (HE = 0.826), and revealed the existence of genetic differentiation among the farms (FST = 2.8 %). The highest inbreeding coefficient was in the Sanjo farm (FIS = 0.303). The results of the parentage testing indicated that all loci showed values greater than 70 % probability of discrimination. However, the highest values found were 94 % (YWLL08) and 90 % (YWLL36). The average of the probability of exclusion obtained was 0.999994 if the genotype for one alleged parents is known, and 0.99999 if the genotypes for both alleged parents are known. The results obtained show that there is a high genetic diversity and validate the panel of microsatellite markers, that would help to improve the identification system and genealogical data collection. 2022-10-26T21:27:54Z 2022-10-26T21:27:54Z 2020-09-22 info:eu-repo/semantics/article Morón, J.; Veli, E.; Memebrillo, A. et al. (2020). Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms. Small Ruminant Research, 193. doi: 10.1016/j.smallrumres.2020.106246 https://hdl.handle.net/20.500.12955/1951 https://doi.org/10.1016/j.smallrumres.2020.106246 eng Small Ruminant Research https://doi.org/10.1016/j.smallrumres.2020.106246 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Perú El Sevier CH Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Microsatellite Alpaca Genetic diversity Parentage testing https://purl.org/pe-repo/ocde/ford#4.04.02 Morón, J. A. Veli Rivera, Eudosio Amancio Membrillo, A. Paredes, M. M. Gutiérrez Reynoso, Gustavo Augusto Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms |
| title | Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms |
| title_full | Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms |
| title_fullStr | Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms |
| title_full_unstemmed | Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms |
| title_short | Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms |
| title_sort | genetic diversity and validation of a microsatellite panel for parentage testing for alpacas vicugna pacos on three peruvian farms |
| topic | Microsatellite Alpaca Genetic diversity Parentage testing https://purl.org/pe-repo/ocde/ford#4.04.02 |
| url | https://hdl.handle.net/20.500.12955/1951 https://doi.org/10.1016/j.smallrumres.2020.106246 |
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