Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing
The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large p...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media S.A
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/1675 https://doi.org/10.3389/fvets.2021.611301 |
| _version_ | 1855490606461091840 |
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| author | Aisen, Eduardo G. Huanca López, Wilfredo Pérez Durand, Manuel G. Torres Mamani, Edita Villanueva Mori, Juan C. Ousset, María J. Medina, Víctor H. Pérez Guerra, Uri Harold Huanca Mamani, Teodosio |
| author_browse | Aisen, Eduardo G. Huanca López, Wilfredo Huanca Mamani, Teodosio Medina, Víctor H. Ousset, María J. Pérez Durand, Manuel G. Pérez Guerra, Uri Harold Torres Mamani, Edita Villanueva Mori, Juan C. |
| author_facet | Aisen, Eduardo G. Huanca López, Wilfredo Pérez Durand, Manuel G. Torres Mamani, Edita Villanueva Mori, Juan C. Ousset, María J. Medina, Víctor H. Pérez Guerra, Uri Harold Huanca Mamani, Teodosio |
| author_sort | Aisen, Eduardo G. |
| collection | Repositorio INIA |
| description | The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted vas deferens. The samples were diluted with a Tris-based extender, packaged in straws, and frozen. At thawing, each straw was divided into two post-thawing conditions: with the addition of 10% of PBS (control) or with 10% SP (treatment). The sperm cells were evaluated using dynamic parameters, sperm cell morphology, and morphometry. Fertility was assessed by an artificial insemination trial. All in vitro parameters were analyzed by ANOVA. A heterogeneity test was scheduled for the fertility trial. After the freezing-thawing process, motility and plasma membrane functionality was improved when SP was added. No differences were found for post-thaw viability between the control and treatment samples. The percentage of normal cells was higher with SP at post-thawing, and a decrease of the presence of bent tailed spermatozoa with a droplet in the SP group was observed. The length of the head spermatozoa was 3.4% higher in the samples with PBS compared to those in which SP was added. Females pregnant at day 25 post-insemination were 0/12 (with SP inside the straw) and 1/10 (without SP inside the straw). In conclusion, the presence of 10% SP at post-thawing improves sperm cells' motility, functionality, and morphology, indicating that it would be beneficial to improve the frozen-thawed alpaca's physiology spermatozoa. More fertility trials must be developed to increase this knowledge. |
| format | Artículo |
| id | INIA1675 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media S.A |
| publisherStr | Frontiers Media S.A |
| record_format | dspace |
| spelling | INIA16752024-12-02T20:21:09Z Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing Aisen, Eduardo G. Huanca López, Wilfredo Pérez Durand, Manuel G. Torres Mamani, Edita Villanueva Mori, Juan C. Ousset, María J. Medina, Víctor H. Pérez Guerra, Uri Harold Huanca Mamani, Teodosio Seminal plasma Alpaca spermatozoa Sperm cryopreservation Vas deferens South American camelids https://purl.org/pe-repo/ocde/ford#4.04.02 The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted vas deferens. The samples were diluted with a Tris-based extender, packaged in straws, and frozen. At thawing, each straw was divided into two post-thawing conditions: with the addition of 10% of PBS (control) or with 10% SP (treatment). The sperm cells were evaluated using dynamic parameters, sperm cell morphology, and morphometry. Fertility was assessed by an artificial insemination trial. All in vitro parameters were analyzed by ANOVA. A heterogeneity test was scheduled for the fertility trial. After the freezing-thawing process, motility and plasma membrane functionality was improved when SP was added. No differences were found for post-thaw viability between the control and treatment samples. The percentage of normal cells was higher with SP at post-thawing, and a decrease of the presence of bent tailed spermatozoa with a droplet in the SP group was observed. The length of the head spermatozoa was 3.4% higher in the samples with PBS compared to those in which SP was added. Females pregnant at day 25 post-insemination were 0/12 (with SP inside the straw) and 1/10 (without SP inside the straw). In conclusion, the presence of 10% SP at post-thawing improves sperm cells' motility, functionality, and morphology, indicating that it would be beneficial to improve the frozen-thawed alpaca's physiology spermatozoa. More fertility trials must be developed to increase this knowledge. Introduction. Materials and methods. Results. Discussion. References. 2022-05-16T17:07:11Z 2022-05-16T17:07:11Z 2021-02-10 info:eu-repo/semantics/article Aisen EG, Huanca López W, Pérez Durand MG, Torres Mamani E, Villanueva Mori JC, Ousset MJ, Medina VH, Pérez Guerra UH and Huanca Mamani T (2021) Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing. Front. Vet. Sci. 8:611301. doi: 10.3389/fvets.2021.611301 https://hdl.handle.net/20.500.12955/1675 Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2021.611301 eng Frontiers in Veterinary Science 8:611301. February 2021 https://doi.org/10.3389/fvets.2021.611301 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Perú Frontiers Media S.A Suiza Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Seminal plasma Alpaca spermatozoa Sperm cryopreservation Vas deferens South American camelids https://purl.org/pe-repo/ocde/ford#4.04.02 Aisen, Eduardo G. Huanca López, Wilfredo Pérez Durand, Manuel G. Torres Mamani, Edita Villanueva Mori, Juan C. Ousset, María J. Medina, Víctor H. Pérez Guerra, Uri Harold Huanca Mamani, Teodosio Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing |
| title | Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing |
| title_full | Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing |
| title_fullStr | Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing |
| title_full_unstemmed | Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing |
| title_short | Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing |
| title_sort | spermatozoa obtained from alpaca vas deferens effects of seminal plasma added at post thawing |
| topic | Seminal plasma Alpaca spermatozoa Sperm cryopreservation Vas deferens South American camelids https://purl.org/pe-repo/ocde/ford#4.04.02 |
| url | https://hdl.handle.net/20.500.12955/1675 https://doi.org/10.3389/fvets.2021.611301 |
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