Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa

A study was designed with the objective of evaluating the effect of seminal plasma on the response to the hypoosmotic swelling test (HOST) in alpaca spermatozoa, for which three experimental groups were organized as follows: Group 1(n=15) plasma free sperm seminal (obtained from the vas deferens, as...

Descripción completa

Detalles Bibliográficos
Autores principales: Pacheco Curie, Joel I., Mamani Cato, Rubén Herberht, Vélez, V. M.
Formato: Artículo
Lenguaje:Inglés
Publicado: MedCrave 2022
Materias:
Acceso en línea:https://hdl.handle.net/20.500.12955/1652
https://doi.org/10.15406/mojbm.2017.02.00059
_version_ 1855490408735309824
author Pacheco Curie, Joel I.
Mamani Cato, Rubén Herberht
Vélez, V. M.
author_browse Mamani Cato, Rubén Herberht
Pacheco Curie, Joel I.
Vélez, V. M.
author_facet Pacheco Curie, Joel I.
Mamani Cato, Rubén Herberht
Vélez, V. M.
author_sort Pacheco Curie, Joel I.
collection Repositorio INIA
description A study was designed with the objective of evaluating the effect of seminal plasma on the response to the hypoosmotic swelling test (HOST) in alpaca spermatozoa, for which three experimental groups were organized as follows: Group 1(n=15) plasma free sperm seminal (obtained from the vas deferens, aspirated in PBS), Group 2(n=15) free seminal plasma sperm reconstituted with seminal plasma (obtained from the vas deferens, aspirated in PBS, mixed in 50/50% with seminal plasma) and Group 3(n=15) whole semen (obtained by artificial vagina), The samples were incubated in a hypoosmotic solution adjusted to 100mOsmol (sodium citrate+fructose+2H2Ocsp 100mL). 0.1mL of semen+0.9mL of hypoosmotic solution was mixed, incubated for 30minutes in a water bath at 37°C and the reaction was stopped with 0.1mL of 4% formaldehyde. A count of at least 200 spermatozoa was performed per sample, using an optical microscope with immersion objective (100X), the vitality was evaluated by supravital eosin staining (0.7%)-nigrosin(1%), the results indicate that it does not exist a detrimental effect of the seminal plasma on the endosmotic response, being, on the contrary, superior in the whole semen; the vitality of the spermatozoa with and without seminal plasma is similar, however it decreases when it is reconstituted with seminal plasma, possibly due to the seminal plasma of another animal; there is no positive correlation between endosmosis and vitality, indicating that the latter would not necessarily reflect the integrity of the membrane, which is why it is recommended to perform this test routinely in alpaca semen exams.
format Artículo
id INIA1652
institution Institucional Nacional de Innovación Agraria
language Inglés
publishDate 2022
publishDateRange 2022
publishDateSort 2022
publisher MedCrave
publisherStr MedCrave
record_format dspace
spelling INIA16522022-11-21T20:07:18Z Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa Pacheco Curie, Joel I. Mamani Cato, Rubén Herberht Vélez, V. M. Endosmosis Seminal plasma Spermatozoon Alpaca https://purl.org/pe-repo/ocde/ford#4.04.02 A study was designed with the objective of evaluating the effect of seminal plasma on the response to the hypoosmotic swelling test (HOST) in alpaca spermatozoa, for which three experimental groups were organized as follows: Group 1(n=15) plasma free sperm seminal (obtained from the vas deferens, aspirated in PBS), Group 2(n=15) free seminal plasma sperm reconstituted with seminal plasma (obtained from the vas deferens, aspirated in PBS, mixed in 50/50% with seminal plasma) and Group 3(n=15) whole semen (obtained by artificial vagina), The samples were incubated in a hypoosmotic solution adjusted to 100mOsmol (sodium citrate+fructose+2H2Ocsp 100mL). 0.1mL of semen+0.9mL of hypoosmotic solution was mixed, incubated for 30minutes in a water bath at 37°C and the reaction was stopped with 0.1mL of 4% formaldehyde. A count of at least 200 spermatozoa was performed per sample, using an optical microscope with immersion objective (100X), the vitality was evaluated by supravital eosin staining (0.7%)-nigrosin(1%), the results indicate that it does not exist a detrimental effect of the seminal plasma on the endosmotic response, being, on the contrary, superior in the whole semen; the vitality of the spermatozoa with and without seminal plasma is similar, however it decreases when it is reconstituted with seminal plasma, possibly due to the seminal plasma of another animal; there is no positive correlation between endosmosis and vitality, indicating that the latter would not necessarily reflect the integrity of the membrane, which is why it is recommended to perform this test routinely in alpaca semen exams. Summary. Introduction. Methodology. Results and discussion. References. 2022-03-28T14:10:05Z 2022-03-28T14:10:05Z 2017-12-22 info:eu-repo/semantics/article Pacheco JI, Cato MRH, Vélez VM. Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa. MOJ Biol Med. 2017;2(4):291–292. doi: 10.15406/mojbm.2017.02.00059 https://hdl.handle.net/20.500.12955/1652 MOJ Biology and Medicine https://doi.org/10.15406/mojbm.2017.02.00059 eng MOJ Biol Med. 2017;2(4):291–292 https://doi.org/10.15406/mojbm.2017.02.00059 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf application/pdf Perú MedCrave Hungría Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA
spellingShingle Endosmosis
Seminal plasma
Spermatozoon
Alpaca
https://purl.org/pe-repo/ocde/ford#4.04.02
Pacheco Curie, Joel I.
Mamani Cato, Rubén Herberht
Vélez, V. M.
Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa
title Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa
title_full Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa
title_fullStr Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa
title_full_unstemmed Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa
title_short Effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa
title_sort effect of seminal plasma on hipoosmotic swelling test in fresh alpaca spermatozoa
topic Endosmosis
Seminal plasma
Spermatozoon
Alpaca
https://purl.org/pe-repo/ocde/ford#4.04.02
url https://hdl.handle.net/20.500.12955/1652
https://doi.org/10.15406/mojbm.2017.02.00059
work_keys_str_mv AT pachecocuriejoeli effectofseminalplasmaonhipoosmoticswellingtestinfreshalpacaspermatozoa
AT mamanicatorubenherberht effectofseminalplasmaonhipoosmoticswellingtestinfreshalpacaspermatozoa
AT velezvm effectofseminalplasmaonhipoosmoticswellingtestinfreshalpacaspermatozoa