Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results
The present study aims to use behavioral responses of the freshwater planarian Girardia tigrina to assess the impact of anthropogenic activities on the aquatic ecosystem of the watershed Araguaia-Tocantins (Tocantins, Brazil). Behavioral responses are integrative and cumulative tools that reflect ch...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2021
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| Online Access: | https://hdl.handle.net/20.500.12955/1475 https://doi.org/10.3390/w13081077 |
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| author | Córdova López, Ana M. De Souza Saraiva, Althiéris Gravato, Carlos Soares, Amadeu M. V. M. Almeida Sarmento, Renato |
| author_browse | Almeida Sarmento, Renato Córdova López, Ana M. De Souza Saraiva, Althiéris Gravato, Carlos Soares, Amadeu M. V. M. |
| author_facet | Córdova López, Ana M. De Souza Saraiva, Althiéris Gravato, Carlos Soares, Amadeu M. V. M. Almeida Sarmento, Renato |
| author_sort | Córdova López, Ana M. |
| collection | Repositorio INIA |
| description | The present study aims to use behavioral responses of the freshwater planarian Girardia tigrina to assess the impact of anthropogenic activities on the aquatic ecosystem of the watershed Araguaia-Tocantins (Tocantins, Brazil). Behavioral responses are integrative and cumulative tools that reflect changes in energy allocation in organisms. Thus, feeding rate and locomotion velocity (pLMV) were determined to assess the effects induced by the laboratory exposure of adult planarians to water samples collected in the region of Tocantins-Araguaia, identifying the sampling points affected by contaminants. Furthermore, physicochemical and microbiological parameters, as well as the presence of inorganic compounds (dissolved aluminum, total barium, total chloride, dissolved iron, total fluoride, total manganese, nitrates, nitric nitrogen, total sulfate, total zinc) and surfactants, were determined on each specific sampling point. The behavioral biomarkers (feeding rate and pLMV) of the freshwater planarians were significantly decreased when organisms were exposed to water samples from four municipalities (Formoso do Araguaia, Lagoa da Confusão, Gurupi and Porto Nacional), sites of the Tocantins-Araguaia hydrographic region—TAHR. Both behavioral biomarkers decreased up to ~37–39% compared to organisms in ASTM medium only. Our results showed that these behavioral biomarkers can be used for fast screening monitoring of environmental samples of freshwater ecosystems, since a decrease in feeding rate and locomotor activity was observed in sites impacted by anthropogenic activities. However, the absence of effects observed in some sampling points does not represent the absence of contamination, since several other classes of contaminants were not determined. In these negative results, the absence of deleterious effects on behavioral biomarkers might only be indicative that the potential presence of contaminants on such sites does not significantly affect the performance of planarians. This fast screening approach seems to be useful to determine contaminated sites in freshwater ecosystems for biomonitoring purposes. This knowledge will help to develop biomonitoring programs and to decide appropriate sampling sites and analysis. |
| format | Artículo |
| id | INIA1475 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INIA14752022-11-21T20:15:02Z Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results Córdova López, Ana M. De Souza Saraiva, Althiéris Gravato, Carlos Soares, Amadeu M. V. M. Almeida Sarmento, Renato Biomonitoring Planarians Locomotion Feeding rate https://purl.org/pe-repo/ocde/ford#4.05.00 The present study aims to use behavioral responses of the freshwater planarian Girardia tigrina to assess the impact of anthropogenic activities on the aquatic ecosystem of the watershed Araguaia-Tocantins (Tocantins, Brazil). Behavioral responses are integrative and cumulative tools that reflect changes in energy allocation in organisms. Thus, feeding rate and locomotion velocity (pLMV) were determined to assess the effects induced by the laboratory exposure of adult planarians to water samples collected in the region of Tocantins-Araguaia, identifying the sampling points affected by contaminants. Furthermore, physicochemical and microbiological parameters, as well as the presence of inorganic compounds (dissolved aluminum, total barium, total chloride, dissolved iron, total fluoride, total manganese, nitrates, nitric nitrogen, total sulfate, total zinc) and surfactants, were determined on each specific sampling point. The behavioral biomarkers (feeding rate and pLMV) of the freshwater planarians were significantly decreased when organisms were exposed to water samples from four municipalities (Formoso do Araguaia, Lagoa da Confusão, Gurupi and Porto Nacional), sites of the Tocantins-Araguaia hydrographic region—TAHR. Both behavioral biomarkers decreased up to ~37–39% compared to organisms in ASTM medium only. Our results showed that these behavioral biomarkers can be used for fast screening monitoring of environmental samples of freshwater ecosystems, since a decrease in feeding rate and locomotor activity was observed in sites impacted by anthropogenic activities. However, the absence of effects observed in some sampling points does not represent the absence of contamination, since several other classes of contaminants were not determined. In these negative results, the absence of deleterious effects on behavioral biomarkers might only be indicative that the potential presence of contaminants on such sites does not significantly affect the performance of planarians. This fast screening approach seems to be useful to determine contaminated sites in freshwater ecosystems for biomonitoring purposes. This knowledge will help to develop biomonitoring programs and to decide appropriate sampling sites and analysis. Abstract. 1. Introduction. 2. Material and Methods. 3. Results. 4. Discussion. 5. Conclusions. References 2021-10-01T22:22:03Z 2021-10-01T22:22:03Z 2021-04-14 info:eu-repo/semantics/article López, A.M.C.; Saraiva, A.d.S.; Gravato, C.; Soares, A.M.V.M.; Sarmento, R.A. Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results. Water 2021, 13, 1077. doi: 10.3390/w13081077 https://hdl.handle.net/20.500.12955/1475 Water https://doi.org/10.3390/w13081077 eng Water 2021, 13(8), 1077 https://doi.org/10.3390/w13081077 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf application/pdf Perú MDPI Suiza Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Biomonitoring Planarians Locomotion Feeding rate https://purl.org/pe-repo/ocde/ford#4.05.00 Córdova López, Ana M. De Souza Saraiva, Althiéris Gravato, Carlos Soares, Amadeu M. V. M. Almeida Sarmento, Renato Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results |
| title | Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results |
| title_full | Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results |
| title_fullStr | Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results |
| title_full_unstemmed | Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results |
| title_short | Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results |
| title_sort | behavioral parameters of planarians girardia tigrina as fast screening integrative and cumulative biomarkers of environmental contamination preliminary results |
| topic | Biomonitoring Planarians Locomotion Feeding rate https://purl.org/pe-repo/ocde/ford#4.05.00 |
| url | https://hdl.handle.net/20.500.12955/1475 https://doi.org/10.3390/w13081077 |
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