Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts
The use of non-commercial and commercial extenders for cooling alpaca sperm has already been reported, the latter showing certain advantages over the first. The Andromed® (AM) extender was created for use in ruminants and has also been tested in ejaculated and epididymal alpaca sperm. According to t...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Austral de Chile
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12955/1455 https://doi.org/10.3389/fvets.2020.597954 |
| _version_ | 1855490506679648256 |
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| author | Bertuzzi, Mariana Lucía Torres, Edita Yola Huanca Mamani, Teodosio Neild, Deborah Carretero, María Ignacia |
| author_browse | Bertuzzi, Mariana Lucía Carretero, María Ignacia Huanca Mamani, Teodosio Neild, Deborah Torres, Edita Yola |
| author_facet | Bertuzzi, Mariana Lucía Torres, Edita Yola Huanca Mamani, Teodosio Neild, Deborah Carretero, María Ignacia |
| author_sort | Bertuzzi, Mariana Lucía |
| collection | Repositorio INIA |
| description | The use of non-commercial and commercial extenders for cooling alpaca sperm has already been reported, the latter showing certain advantages over the first. The Andromed® (AM) extender was created for use in ruminants and has also been tested in ejaculated and epididymal alpaca sperm. According to the manufacturer, this extender does not need the addition of egg yolk (EY); however, it is known that the addition of EY to some extenders improves the preservation of cooled sperm. The objective of this study therefore was to compare a non-commercial extender (Tris) with the addition of EY vs. the commercial extender AM with and without the addition of EY, for cooling alpaca sperm obtained from diverted deferent ducts. Fifteen pools of deferent duct sperm were formed using samples from two or three different males for each. Each sperm pool was evaluated and then divided into three aliquots that were diluted to a final concentration of 30 × 106 sperm ml-1 (0 h) with either: (1) Tris with 20% EY (T-EY), (2) AM, or (3) AM with 20% EY (AM-EY). Samples were cooled to 5°C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, acrosome integrity, morphology, and chromatin condensation. Motility was also evaluated after 72 h of storage. The samples that best preserved progressive and total sperm motility at the 24 and 48 h evaluation periods were the ones diluted with AM-EY, observing that with this extender these motility patterns decreased significantly after 72 h of storage compared to time 0 h (p < 0.05). A significant decrease (p < 0.05) in total and progressive motility was observed at 48 h for the T-EY and AM extender compared to 0 h. AM was the only extender in which the percentages of viable sperm decreased significantly (p < 0.05) after 48 h of conservation. For the rest of sperm parameters evaluated, no significant differences were observed between any of the extenders at any evaluation time. The Andromed® extender with the addition of 20% EY could be an alternative option for cooling alpaca sperm obtained from deferent ducts. |
| format | Artículo |
| id | INIA1455 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Universidad Austral de Chile |
| publisherStr | Universidad Austral de Chile |
| record_format | dspace |
| spelling | INIA14552022-11-21T20:15:51Z Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts Bertuzzi, Mariana Lucía Torres, Edita Yola Huanca Mamani, Teodosio Neild, Deborah Carretero, María Ignacia Extenders Cooling Egg Yolk Sperm Alpaca Deferent ducts https://purl.org/pe-repo/ocde/ford#4.03.01 The use of non-commercial and commercial extenders for cooling alpaca sperm has already been reported, the latter showing certain advantages over the first. The Andromed® (AM) extender was created for use in ruminants and has also been tested in ejaculated and epididymal alpaca sperm. According to the manufacturer, this extender does not need the addition of egg yolk (EY); however, it is known that the addition of EY to some extenders improves the preservation of cooled sperm. The objective of this study therefore was to compare a non-commercial extender (Tris) with the addition of EY vs. the commercial extender AM with and without the addition of EY, for cooling alpaca sperm obtained from diverted deferent ducts. Fifteen pools of deferent duct sperm were formed using samples from two or three different males for each. Each sperm pool was evaluated and then divided into three aliquots that were diluted to a final concentration of 30 × 106 sperm ml-1 (0 h) with either: (1) Tris with 20% EY (T-EY), (2) AM, or (3) AM with 20% EY (AM-EY). Samples were cooled to 5°C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, acrosome integrity, morphology, and chromatin condensation. Motility was also evaluated after 72 h of storage. The samples that best preserved progressive and total sperm motility at the 24 and 48 h evaluation periods were the ones diluted with AM-EY, observing that with this extender these motility patterns decreased significantly after 72 h of storage compared to time 0 h (p < 0.05). A significant decrease (p < 0.05) in total and progressive motility was observed at 48 h for the T-EY and AM extender compared to 0 h. AM was the only extender in which the percentages of viable sperm decreased significantly (p < 0.05) after 48 h of conservation. For the rest of sperm parameters evaluated, no significant differences were observed between any of the extenders at any evaluation time. The Andromed® extender with the addition of 20% EY could be an alternative option for cooling alpaca sperm obtained from deferent ducts. INTRODUCTION. MATERIALES AND METHODS. RESULTS. DISCUSSION. CONCLUSIONS. REFERENCES 2021-08-31T20:18:59Z 2021-08-31T20:18:59Z 2020-11-30 info:eu-repo/semantics/article Bertuzzi ML, Torres EY, Huanca T, Neild D and Carretero MI (2020) Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts. Front. Vet. Sci. 7:597954. doi: 10.3389/fvets.2020.597954 https://hdl.handle.net/20.500.12955/1455 Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2020.597954 eng Frontiers in Veterinary Science 7:59794 https://doi.org/10.3389/fvets.2020.597954 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf application/pdf Perú Universidad Austral de Chile Suiza Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Extenders Cooling Egg Yolk Sperm Alpaca Deferent ducts https://purl.org/pe-repo/ocde/ford#4.03.01 Bertuzzi, Mariana Lucía Torres, Edita Yola Huanca Mamani, Teodosio Neild, Deborah Carretero, María Ignacia Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts |
| title | Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts |
| title_full | Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts |
| title_fullStr | Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts |
| title_full_unstemmed | Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts |
| title_short | Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts |
| title_sort | comparison of extenders with the addition of egg yolk for cooling alpaca sperm obtained from deferent ducts |
| topic | Extenders Cooling Egg Yolk Sperm Alpaca Deferent ducts https://purl.org/pe-repo/ocde/ford#4.03.01 |
| url | https://hdl.handle.net/20.500.12955/1455 https://doi.org/10.3389/fvets.2020.597954 |
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