Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
9 Páginas
| Autores principales: | , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Nacional de Trujillo
2021
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| Materias: | |
| Acceso en línea: | https://repositorio.inia.gob.pe/handle/20.500.12955/1301 https://doi.org/10.17268/sci.agropecu.2021.011 |
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| author | Gutiérrez Calle, Savina Alejandra Sánchez Díaz, Rosa A. Delgado Silva, Yolanda Bedsabé Montenegro, Juan D. Gutiérrez Reynoso, Dina Lida Maicelo Quintana, Jorge Luis Guerrero Abad, Juan Carlos |
| author_browse | Delgado Silva, Yolanda Bedsabé Guerrero Abad, Juan Carlos Gutiérrez Calle, Savina Alejandra Gutiérrez Reynoso, Dina Lida Maicelo Quintana, Jorge Luis Montenegro, Juan D. Sánchez Díaz, Rosa A. |
| author_facet | Gutiérrez Calle, Savina Alejandra Sánchez Díaz, Rosa A. Delgado Silva, Yolanda Bedsabé Montenegro, Juan D. Gutiérrez Reynoso, Dina Lida Maicelo Quintana, Jorge Luis Guerrero Abad, Juan Carlos |
| author_sort | Gutiérrez Calle, Savina Alejandra |
| collection | Repositorio INIA |
| description | 9 Páginas |
| format | info:eu-repo/semantics/article |
| id | INIA1301 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Universidad Nacional de Trujillo |
| publisherStr | Universidad Nacional de Trujillo |
| record_format | dspace |
| spelling | INIA13012022-11-18T16:46:05Z Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance Gutiérrez Calle, Savina Alejandra Sánchez Díaz, Rosa A. Delgado Silva, Yolanda Bedsabé Montenegro, Juan D. Gutiérrez Reynoso, Dina Lida Maicelo Quintana, Jorge Luis Guerrero Abad, Juan Carlos Coffee Capillary Electrophoresis Hemileia vastatrix SH3 Biotecnología agrícola, Biotecnología alimentaria 9 Páginas Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The SH3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the SH3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of SH3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the SH3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to SH3 was demonstrated. 1. Introduction. 2. Materials and methods. 3. Results and discussion. 4. Conclusions. References. 2021-03-15T18:30:46Z 2021-03-15T18:30:46Z 2021-03-10 info:eu-repo/semantics/article Gutiérrez-Calle, S. A. ., Sánchez-Díaz, R. A. ., Delgado-Silva, Y. B. ., Montenegro, J. D. ., Gutiérrez, D. L. ., Maicelo-Quintana, J. L. ., & Guerrero-Abad, J. C. . (2021). Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance. Scientia Agropecuaria, 12(1), 91-99. doi: 10.17268/sci.agropecu.2021.011 https://repositorio.inia.gob.pe/handle/20.500.12955/1301 Scientia Agropecuaria https://doi.org/10.17268/sci.agropecu.2021.011 eng Scientia Agropecuaria. Vol. 12 Núm. 1 (2021). 91-99 https://doi.org/10.17268/sci.agropecu.2021.011 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Perú Universidad Nacional de Trujillo Perú Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Coffee Capillary Electrophoresis Hemileia vastatrix SH3 Biotecnología agrícola, Biotecnología alimentaria Gutiérrez Calle, Savina Alejandra Sánchez Díaz, Rosa A. Delgado Silva, Yolanda Bedsabé Montenegro, Juan D. Gutiérrez Reynoso, Dina Lida Maicelo Quintana, Jorge Luis Guerrero Abad, Juan Carlos Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_full | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_fullStr | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_full_unstemmed | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_short | Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance |
| title_sort | capillary electrophoresis as a tool for genotyping sh3 mediated coffee leaf rust resistance |
| topic | Coffee Capillary Electrophoresis Hemileia vastatrix SH3 Biotecnología agrícola, Biotecnología alimentaria |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1301 https://doi.org/10.17268/sci.agropecu.2021.011 |
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