From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa

The tRNAleu-COX2 intergenic region of the mitochondrial DNA has been used for assessing diversity in honey bee (Apis mellifera L.) populations worldwide. However, differential mutation rates in different partitions of the mitogenome may produce incongruent results. In this study, we sequenced 123 mi...

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Autores principales: Henriques, Dora, Chávez Galarza, Julio César, Quaresma, Andreia, Neves, Cátia José, Lopes, Ana Rita, Costa, Cecília, Costa, Filipe O., Rufino, José, Pinto, María Alice
Formato: info:eu-repo/semantics/article
Lenguaje:Inglés
Publicado: Springer Nature 2021
Materias:
Acceso en línea:https://repositorio.inia.gob.pe/handle/20.500.12955/1228
https://doi.org/10.1007/s13592-019-00632-9
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author Henriques, Dora
Chávez Galarza, Julio César
Quaresma, Andreia
Neves, Cátia José
Lopes, Ana Rita
Costa, Cecília
Costa, Filipe O.
Rufino, José
Pinto, María Alice
author_browse Chávez Galarza, Julio César
Costa, Cecília
Costa, Filipe O.
Henriques, Dora
Lopes, Ana Rita
Neves, Cátia José
Pinto, María Alice
Quaresma, Andreia
Rufino, José
author_facet Henriques, Dora
Chávez Galarza, Julio César
Quaresma, Andreia
Neves, Cátia José
Lopes, Ana Rita
Costa, Cecília
Costa, Filipe O.
Rufino, José
Pinto, María Alice
author_sort Henriques, Dora
collection Repositorio INIA
description The tRNAleu-COX2 intergenic region of the mitochondrial DNA has been used for assessing diversity in honey bee (Apis mellifera L.) populations worldwide. However, differential mutation rates in different partitions of the mitogenome may produce incongruent results. In this study, we sequenced 123 mitogenomes of 7 subspecies from lineages A, M, and C. This allowed generating a comprehensive dataset to investigate the phylogenetic and phylogeographic congruence among the mitogenome, individual genes, and the tRNAleu-COX2 region. We showed that the diversity patterns inferred from the tRNAleu-COX2 marker are not fully paralleled by those obtained with the mitogenome and the individual genes; while the three lineages are supported by these, the African sub-lineages and the haplotypes are not. Thus, conclusions drawn from the tRNAleu-COX2 region need to be taken with caution and this marker may not be appropriate to infer phylogenetic relationships between honey bee colonies.
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spelling INIA12282022-11-21T20:25:37Z From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa Henriques, Dora Chávez Galarza, Julio César Quaresma, Andreia Neves, Cátia José Lopes, Ana Rita Costa, Cecília Costa, Filipe O. Rufino, José Pinto, María Alice Iberian honey bee tRNA(leu)-COX2 Intergenic region Mitogenome Tecnología de modificación genética The tRNAleu-COX2 intergenic region of the mitochondrial DNA has been used for assessing diversity in honey bee (Apis mellifera L.) populations worldwide. However, differential mutation rates in different partitions of the mitogenome may produce incongruent results. In this study, we sequenced 123 mitogenomes of 7 subspecies from lineages A, M, and C. This allowed generating a comprehensive dataset to investigate the phylogenetic and phylogeographic congruence among the mitogenome, individual genes, and the tRNAleu-COX2 region. We showed that the diversity patterns inferred from the tRNAleu-COX2 marker are not fully paralleled by those obtained with the mitogenome and the individual genes; while the three lineages are supported by these, the African sub-lineages and the haplotypes are not. Thus, conclusions drawn from the tRNAleu-COX2 region need to be taken with caution and this marker may not be appropriate to infer phylogenetic relationships between honey bee colonies. 1. INTRODUCTION. 2. METHODS. 3. RESULTS. 4. DISCUSSION. REFERENCES 2021-01-19T13:45:23Z 2021-01-19T13:45:23Z 2019-03-01 info:eu-repo/semantics/article Henriques, D., Chávez-Galarza, J., Quaresma, A. et al. From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa Apidologie 50, 215–229 (2019). doi: 10.1007/s13592-019-00632-9 https://repositorio.inia.gob.pe/handle/20.500.12955/1228 Apidologie https://doi.org/10.1007/s13592-019-00632-9 eng Apidologie (2019) 50:215–229 https://doi.org/10.1007/s13592-019-00632-9 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Europa y North Africa Springer Nature Reino Unido Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA
spellingShingle Iberian honey bee
tRNA(leu)-COX2
Intergenic region
Mitogenome
Tecnología de modificación genética
Henriques, Dora
Chávez Galarza, Julio César
Quaresma, Andreia
Neves, Cátia José
Lopes, Ana Rita
Costa, Cecília
Costa, Filipe O.
Rufino, José
Pinto, María Alice
From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa
title From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa
title_full From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa
title_fullStr From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa
title_full_unstemmed From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa
title_short From the popular tRNAleu-COX2 intergenic region to the mitogenome: insights from diverse honey bee populations of Europe and North Africa
title_sort from the popular trnaleu cox2 intergenic region to the mitogenome insights from diverse honey bee populations of europe and north africa
topic Iberian honey bee
tRNA(leu)-COX2
Intergenic region
Mitogenome
Tecnología de modificación genética
url https://repositorio.inia.gob.pe/handle/20.500.12955/1228
https://doi.org/10.1007/s13592-019-00632-9
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