Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula

The availability of powerful high-throughput genomic tools, combined with genome scans, has helped identifying genes and genetic changes responsible for environmental adaptation in many organisms, including the honeybee. Here, we resequenced 87 whole genomes of the honeybee native to Iberia and used...

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Autores principales: Henriques, Dora, Wallberg, Andreas, Chávez Galarza, Julio César, Johnston, J. Spencer, Webster, Matthew, Pinto, María Alice
Formato: info:eu-repo/semantics/article
Lenguaje:Inglés
Publicado: Springer Nature 2021
Materias:
Acceso en línea:https://repositorio.inia.gob.pe/handle/20.500.12955/1223
https://doi.org/10.1038/s41598-018-29469-5
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author Henriques, Dora
Wallberg, Andreas
Chávez Galarza, Julio César
Johnston, J. Spencer
Webster, Matthew
Pinto, María Alice
author_browse Chávez Galarza, Julio César
Henriques, Dora
Johnston, J. Spencer
Pinto, María Alice
Wallberg, Andreas
Webster, Matthew
author_facet Henriques, Dora
Wallberg, Andreas
Chávez Galarza, Julio César
Johnston, J. Spencer
Webster, Matthew
Pinto, María Alice
author_sort Henriques, Dora
collection Repositorio INIA
description The availability of powerful high-throughput genomic tools, combined with genome scans, has helped identifying genes and genetic changes responsible for environmental adaptation in many organisms, including the honeybee. Here, we resequenced 87 whole genomes of the honeybee native to Iberia and used conceptually different selection methods (Samβada, LFMM, PCAdapt, iHs) together with in sillico protein modelling to search for selection footprints along environmental gradients. We found 670 outlier SNPs, most of which associated with precipitation, longitude and latitude. Over 88.7% SNPs laid outside exons and there was a significant enrichment in regions adjacent to exons and UTRs. Enrichment was also detected in exonic regions. Furthermore, in silico protein modelling suggests that several non-synonymous SNPs are likely direct targets of selection, as they lead to amino acid replacements in functionally important sites of proteins. We identified genomic signatures of local adaptation in 140 genes, many of which are putatively implicated in fitness-related functions such as reproduction, immunity, olfaction, lipid biosynthesis and circadian clock. Our genome scan suggests that local adaptation in the Iberian honeybee involves variations in regions that might alter patterns of gene expression and in protein-coding genes, which are promising candidates to underpin adaptive change in the honeybee.
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spelling INIA12232022-11-21T20:26:24Z Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula Henriques, Dora Wallberg, Andreas Chávez Galarza, Julio César Johnston, J. Spencer Webster, Matthew Pinto, María Alice Honeybee Apis mellifera L. Iberian Peninsula Genome SNP-associated Genes Otras ciencias agrícolas The availability of powerful high-throughput genomic tools, combined with genome scans, has helped identifying genes and genetic changes responsible for environmental adaptation in many organisms, including the honeybee. Here, we resequenced 87 whole genomes of the honeybee native to Iberia and used conceptually different selection methods (Samβada, LFMM, PCAdapt, iHs) together with in sillico protein modelling to search for selection footprints along environmental gradients. We found 670 outlier SNPs, most of which associated with precipitation, longitude and latitude. Over 88.7% SNPs laid outside exons and there was a significant enrichment in regions adjacent to exons and UTRs. Enrichment was also detected in exonic regions. Furthermore, in silico protein modelling suggests that several non-synonymous SNPs are likely direct targets of selection, as they lead to amino acid replacements in functionally important sites of proteins. We identified genomic signatures of local adaptation in 140 genes, many of which are putatively implicated in fitness-related functions such as reproduction, immunity, olfaction, lipid biosynthesis and circadian clock. Our genome scan suggests that local adaptation in the Iberian honeybee involves variations in regions that might alter patterns of gene expression and in protein-coding genes, which are promising candidates to underpin adaptive change in the honeybee. Abstract. Introduction. Results. Discussion. Methods. References 2021-01-12T15:19:15Z 2021-01-12T15:19:15Z 2018-07-24 info:eu-repo/semantics/article Henriques, D., Wallberg, A., Chávez-Galarza, J. et al. Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula. Sci Rep 8, 11145 (2018). doi: 10.1038/s41598-018-29469-5 https://repositorio.inia.gob.pe/handle/20.500.12955/1223 Scientific Reports https://doi.org/10.1038/s41598-018-29469-5 eng Scientific Reports (2018) 8:11145 https://doi.org/10.1038/s41598-018-29469-5 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Península Ibérica Springer Nature Reino Unido Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA
spellingShingle Honeybee
Apis mellifera L.
Iberian Peninsula
Genome SNP-associated
Genes
Otras ciencias agrícolas
Henriques, Dora
Wallberg, Andreas
Chávez Galarza, Julio César
Johnston, J. Spencer
Webster, Matthew
Pinto, María Alice
Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula
title Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula
title_full Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula
title_fullStr Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula
title_full_unstemmed Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula
title_short Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula
title_sort whole genome snp associated signatures of local adaptation in honeybees of the iberian peninsula
topic Honeybee
Apis mellifera L.
Iberian Peninsula
Genome SNP-associated
Genes
Otras ciencias agrícolas
url https://repositorio.inia.gob.pe/handle/20.500.12955/1223
https://doi.org/10.1038/s41598-018-29469-5
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