Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia
The aim of this work was to elucidate the molecular and biochemical mechanisms that control L-ascorbic acid (AsA) content variation in Myrciaria dubia. The AsA was quantified by high-performance liquid chromatography, gene expression by real-time quantitative PCR, and enzyme activities by spectropho...
| Autores principales: | , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2021
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| Materias: | |
| Acceso en línea: | https://repositorio.inia.gob.pe/handle/20.500.12955/1218 https://doi.org/10.1007/s10535-015-0540-z |
| _version_ | 1855028707070050304 |
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| author | Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Egoavil Reátegui, Alina del Carmen Torres, Juan Gutierrez, F. |
| author_browse | Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Egoavil Reátegui, Alina del Carmen Gutierrez, F. Imán Correa, Sixto Alfredo Maddox, J. Dylan Torres, Juan |
| author_facet | Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Egoavil Reátegui, Alina del Carmen Torres, Juan Gutierrez, F. |
| author_sort | Castro Gómez, Juan Carlos |
| collection | Repositorio INIA |
| description | The aim of this work was to elucidate the molecular and biochemical mechanisms that control L-ascorbic acid (AsA) content variation in Myrciaria dubia. The AsA was quantified by high-performance liquid chromatography, gene expression by real-time quantitative PCR, and enzyme activities by spectrophotometric methods from leaves and immature fruits of two genotypes (Md-60,06 and Md-02,04) with pronounced (about 2 times) differences in the AsA content. In either genotype, the fruit peel had ∼ 1.5 times more AsA than the fruit pulp and ∼ 15.0 times more than the leaf. All tissues examined demonstrated the capability for AsA biosynthesis through the D-mannose/L-galactose pathway because mRNAs of the six key genes [GDP-D-mannose pyrophosphorylase (GMP), GDP-D-mannose-3′,5′-epimerase (GME), GDP-L-galactose phosphorylase (GGP), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH), and L-galactono-1-4-lactone dehydrogenase (GLDH)] and catalytic activities of the corresponding enzymes (GMP, GDH, and GLDH) were detected. The differential expressions of genes and enzyme activities mostly correlated with the respective AsA content. Thus, the expression of several genes of the D-mannose/L-galactose pathway determined the AsA content variation in tissues of M. dubia. |
| format | info:eu-repo/semantics/article |
| id | INIA1218 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INIA12182023-10-11T15:17:48Z Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Egoavil Reátegui, Alina del Carmen Torres, Juan Gutierrez, F. GDP-D-mannose-3′,5′-epimerase GDP-D-mannose pyrophosphorylase GDP-L-galactose phosphorylase L-galactono-1-4-lactone dehydrogenase L-galactose dehydrogenase L-galactose-1-phosphate phosphatase Biotecnología agrícola, Biotecnología alimentaria The aim of this work was to elucidate the molecular and biochemical mechanisms that control L-ascorbic acid (AsA) content variation in Myrciaria dubia. The AsA was quantified by high-performance liquid chromatography, gene expression by real-time quantitative PCR, and enzyme activities by spectrophotometric methods from leaves and immature fruits of two genotypes (Md-60,06 and Md-02,04) with pronounced (about 2 times) differences in the AsA content. In either genotype, the fruit peel had ∼ 1.5 times more AsA than the fruit pulp and ∼ 15.0 times more than the leaf. All tissues examined demonstrated the capability for AsA biosynthesis through the D-mannose/L-galactose pathway because mRNAs of the six key genes [GDP-D-mannose pyrophosphorylase (GMP), GDP-D-mannose-3′,5′-epimerase (GME), GDP-L-galactose phosphorylase (GGP), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH), and L-galactono-1-4-lactone dehydrogenase (GLDH)] and catalytic activities of the corresponding enzymes (GMP, GDH, and GLDH) were detected. The differential expressions of genes and enzyme activities mostly correlated with the respective AsA content. Thus, the expression of several genes of the D-mannose/L-galactose pathway determined the AsA content variation in tissues of M. dubia. 2021-01-12T15:14:01Z 2021-01-12T15:14:01Z 2015-12-01 info:eu-repo/semantics/article Castro, J.C., Cobos, M., Maddox, J.D. et al. Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia . Biol Plant 59, 783–787 (2015). doi: 10.1007/s10535-015-0540-z https://repositorio.inia.gob.pe/handle/20.500.12955/1218 BIOLOGIA PLANTARUM https://doi.org/10.1007/s10535-015-0540-z eng BIOLOGIA PLANTARUM 59 (4): 783-787, 2015 https://doi.org/10.1007/s10535-015-0540-z info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Perú Springer Nature Chequia Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | GDP-D-mannose-3′,5′-epimerase GDP-D-mannose pyrophosphorylase GDP-L-galactose phosphorylase L-galactono-1-4-lactone dehydrogenase L-galactose dehydrogenase L-galactose-1-phosphate phosphatase Biotecnología agrícola, Biotecnología alimentaria Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Egoavil Reátegui, Alina del Carmen Torres, Juan Gutierrez, F. Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title | Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_full | Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_fullStr | Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_full_unstemmed | Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_short | Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia |
| title_sort | gene expression and enzyme activities of the d mannose l galactose pathway influence l ascorbic acid content in myrciaria dubia |
| topic | GDP-D-mannose-3′,5′-epimerase GDP-D-mannose pyrophosphorylase GDP-L-galactose phosphorylase L-galactono-1-4-lactone dehydrogenase L-galactose dehydrogenase L-galactose-1-phosphate phosphatase Biotecnología agrícola, Biotecnología alimentaria |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1218 https://doi.org/10.1007/s10535-015-0540-z |
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