Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association
Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on...
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| Format: | Artículo |
| Language: | Inglés |
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Universidad Politécnica de Valencia
2020
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| Online Access: | https://repositorio.inia.gob.pe/handle/20.500.12955/1193 https://doi.org/10.3389/fpls.2015.00402 |
| _version_ | 1855490384204922880 |
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| author | Castillo, Ana M. Sánchez Díaz, Rosa Angélica Vallés, María P. |
| author_browse | Castillo, Ana M. Sánchez Díaz, Rosa Angélica Vallés, María P. |
| author_facet | Castillo, Ana M. Sánchez Díaz, Rosa Angélica Vallés, María P. |
| author_sort | Castillo, Ana M. |
| collection | Repositorio INIA |
| description | Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected microspore embryogenesis. By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism. Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25–46% as compared to immature ovaries (excised from flowers with microspores at a mid-late uninucleate stage). The highest numbers of embryos and green plants were produced when using mature Caramba ovaries. Ovaries from Galeón, Tigre, and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries. Moreover, Tigre ovaries raised the percentage of spontaneous chromosome doubling up to 71%. Attempts were made to identify molecular mechanisms associated to the inductive effect of the ovaries on microspore embryogenesis. The genes TAA1b, FLA26, and WALI6 associated to wheat microspore embryogenesis, the CGL1 gene involved in glycan biosynthesis or degradation, and the FER gene involved in the ovary signaling process were expressed and/or induced at different rates during ovary culture. The expression pattern of FLA26 and FER could be related to the differences between genotypes and developmental stages in the inductive effect of the ovary. Our results open opportunities for new approaches to increase bread wheat doubled haploid production by anther culture, and to identify the functional components of the ovary inductive effect on microspore embryogenesis. |
| format | Artículo |
| id | INIA1193 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Universidad Politécnica de Valencia |
| publisherStr | Universidad Politécnica de Valencia |
| record_format | dspace |
| spelling | INIA11932023-12-22T13:39:21Z Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association Castillo, Ana M. Sánchez Díaz, Rosa Angélica Vallés, María P. Wheat Antherculture Ovaryco-culture Ovary developmental stage Ovarygenotype Fasciclin-like Arabinogalactan FERONIA Tecnología de modificación genética Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected microspore embryogenesis. By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism. Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25–46% as compared to immature ovaries (excised from flowers with microspores at a mid-late uninucleate stage). The highest numbers of embryos and green plants were produced when using mature Caramba ovaries. Ovaries from Galeón, Tigre, and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries. Moreover, Tigre ovaries raised the percentage of spontaneous chromosome doubling up to 71%. Attempts were made to identify molecular mechanisms associated to the inductive effect of the ovaries on microspore embryogenesis. The genes TAA1b, FLA26, and WALI6 associated to wheat microspore embryogenesis, the CGL1 gene involved in glycan biosynthesis or degradation, and the FER gene involved in the ovary signaling process were expressed and/or induced at different rates during ovary culture. The expression pattern of FLA26 and FER could be related to the differences between genotypes and developmental stages in the inductive effect of the ovary. Our results open opportunities for new approaches to increase bread wheat doubled haploid production by anther culture, and to identify the functional components of the ovary inductive effect on microspore embryogenesis. Introduction. Materials and Methods. Results. Discussion. References 2020-12-01T20:27:21Z 2020-12-01T20:27:21Z 2015-06-18 info:eu-repo/semantics/article Castillo, A.; Sánchez, R. & Vallés, M. (2015). Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association. Front. PlantSci.6:402. doi: 10.3389/fpls.2015.00402 https://repositorio.inia.gob.pe/handle/20.500.12955/1193 Fontiers in Plant Science https://doi.org/10.3389/fpls.2015.00402 eng Front. PlantSci. 18 June 2015, 6:402 https://doi.org/10.3389/fpls.2015.00402 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf España Universidad Politécnica de Valencia España Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Wheat Antherculture Ovaryco-culture Ovary developmental stage Ovarygenotype Fasciclin-like Arabinogalactan FERONIA Tecnología de modificación genética Castillo, Ana M. Sánchez Díaz, Rosa Angélica Vallés, María P. Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association |
| title | Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association |
| title_full | Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association |
| title_fullStr | Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association |
| title_full_unstemmed | Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association |
| title_short | Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association |
| title_sort | effect of ovary induction on bread wheat anther culture ovary genotype and developmental stage and candidate gene association |
| topic | Wheat Antherculture Ovaryco-culture Ovary developmental stage Ovarygenotype Fasciclin-like Arabinogalactan FERONIA Tecnología de modificación genética |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1193 https://doi.org/10.3389/fpls.2015.00402 |
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