Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination
The evolutionary divergence of Potato mop-top virus (PMTV), a tri-partite, single-stranded RNA virus, is exceptionally low, based on the analysis of sequences obtained from isolates from Europe, Asia and North America. In general, RNA viruses exist as Dynamic populations of closely related and recom...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
British Society for Plant Pathology
2020
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| Materias: | |
| Acceso en línea: | https://repositorio.inia.gob.pe/handle/20.500.12955/1185 https://doi.org/10.1111/mpp.12552 |
| _version_ | 1855490285505609728 |
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| author | Kaltandurg, Pruthvi Gil, José Fernando Lukhovitskaya, Nina I. Flores, Betty Müller, Giovanna Chuquillanqui, Carlos Palomino Flores, Ladislao Monjane, Aderito Barker, Ian Kreuze, Jan Savenkov, Eugene I. |
| author_browse | Barker, Ian Chuquillanqui, Carlos Flores, Betty Gil, José Fernando Kaltandurg, Pruthvi Kreuze, Jan Lukhovitskaya, Nina I. Monjane, Aderito Müller, Giovanna Palomino Flores, Ladislao Savenkov, Eugene I. |
| author_facet | Kaltandurg, Pruthvi Gil, José Fernando Lukhovitskaya, Nina I. Flores, Betty Müller, Giovanna Chuquillanqui, Carlos Palomino Flores, Ladislao Monjane, Aderito Barker, Ian Kreuze, Jan Savenkov, Eugene I. |
| author_sort | Kaltandurg, Pruthvi |
| collection | Repositorio INIA |
| description | The evolutionary divergence of Potato mop-top virus (PMTV), a tri-partite, single-stranded RNA virus, is exceptionally low, based on the analysis of sequences obtained from isolates from Europe, Asia and North America. In general, RNA viruses exist as Dynamic populations of closely related and recombinant genomes that are subjected to continuous genetic variation. The reason behind the low genetic variation of PMTV remains unclear. The question remains as to whether the low variability is a shared property of all PMTV isolates or is a result of the limited number of isolates characterized so far. We hypothesized that higher divergence of the virus might exist in the Andean regions of South America, the centre of potato domestication. Here, we report high variability of PMTV isolates collected from 12 fields in three locations in the Andean region of Peru. To evaluate PMTV genetic variation in Peru, we generated full-length cDNA clones, which allowed reliable comparative molecular and pathobiological characterization of individual isolates. We found significant divergence of the CP-RT and 8K sequences. The 8K cistron, which encodes a viral suppressor of RNA silencing, was found to be under diversifying selection. Phylogenetic analysis determined that, based on the CP-RT sequence, all PMTV isolates could be categorized into three separate lineages (clades). Moreover, we found evidence for recombination between two clades. Using infectious cDNA clones of the representatives of these two clades, as well as reassortants for the RNA-CP genomic component, we determined the pathobiological differences between the lineages, which we coined as S (for severe) and M (for mild) types. Interestingly, all isolates characterized previously (from Europe, Asia and North America) fall into the S-type clade, whereas most of the Peruvian isolates belong to the M-type. Taken together, our results support the notion of the single introduction of PMTV from the centre of potato origin to Europe, and subsequent spread of the S-type into Asia and USA. This is also supported by the suggested novel classification of isolates based on genetic constellations. |
| format | Artículo |
| id | INIA1185 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | British Society for Plant Pathology |
| publisherStr | British Society for Plant Pathology |
| record_format | dspace |
| spelling | INIA11852022-11-21T20:31:28Z Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination Kaltandurg, Pruthvi Gil, José Fernando Lukhovitskaya, Nina I. Flores, Betty Müller, Giovanna Chuquillanqui, Carlos Palomino Flores, Ladislao Monjane, Aderito Barker, Ian Kreuze, Jan Savenkov, Eugene I. Diversifying selection Evolutionary divergence Genotype constellation Infectious full-length cDNA clone Multipartite virus Potato mop-top virus Reassortment Tecnología de modificación genética The evolutionary divergence of Potato mop-top virus (PMTV), a tri-partite, single-stranded RNA virus, is exceptionally low, based on the analysis of sequences obtained from isolates from Europe, Asia and North America. In general, RNA viruses exist as Dynamic populations of closely related and recombinant genomes that are subjected to continuous genetic variation. The reason behind the low genetic variation of PMTV remains unclear. The question remains as to whether the low variability is a shared property of all PMTV isolates or is a result of the limited number of isolates characterized so far. We hypothesized that higher divergence of the virus might exist in the Andean regions of South America, the centre of potato domestication. Here, we report high variability of PMTV isolates collected from 12 fields in three locations in the Andean region of Peru. To evaluate PMTV genetic variation in Peru, we generated full-length cDNA clones, which allowed reliable comparative molecular and pathobiological characterization of individual isolates. We found significant divergence of the CP-RT and 8K sequences. The 8K cistron, which encodes a viral suppressor of RNA silencing, was found to be under diversifying selection. Phylogenetic analysis determined that, based on the CP-RT sequence, all PMTV isolates could be categorized into three separate lineages (clades). Moreover, we found evidence for recombination between two clades. Using infectious cDNA clones of the representatives of these two clades, as well as reassortants for the RNA-CP genomic component, we determined the pathobiological differences between the lineages, which we coined as S (for severe) and M (for mild) types. Interestingly, all isolates characterized previously (from Europe, Asia and North America) fall into the S-type clade, whereas most of the Peruvian isolates belong to the M-type. Taken together, our results support the notion of the single introduction of PMTV from the centre of potato origin to Europe, and subsequent spread of the S-type into Asia and USA. This is also supported by the suggested novel classification of isolates based on genetic constellations. SUMMARY. INTRODUCTION. RESULTS. DISCUSSION. EXPERIMENTAL PROCEDURES. REFERENCES 2020-11-18T21:34:47Z 2020-11-18T21:34:47Z 2017-04-08 info:eu-repo/semantics/article Kalyandurg, P., Gil, J.F., Lukhovitskaya, N.I., Flores, B., Müller, G., Chuquillanqui, C., Palomino, L., Monjane, A., Barker, I., Kreuze, J. and Savenkov, E.I. (2017), Molecular and pathobiological characterization of 61 Potato mop‐top virus full‐length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination. Molecular Plant Pathology, 18: 864-877. doi:10.1111/mpp.12552 https://repositorio.inia.gob.pe/handle/20.500.12955/1185 MOLECULAR PLANT PATHOLOGY https://doi.org/10.1111/mpp.12552 eng MOLECULAR PLANT PATHOLOGY (2017) 18(6) , 864–877 https://doi.org/10.1111/mpp.12552 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Perú British Society for Plant Pathology Reino Unido Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Diversifying selection Evolutionary divergence Genotype constellation Infectious full-length cDNA clone Multipartite virus Potato mop-top virus Reassortment Tecnología de modificación genética Kaltandurg, Pruthvi Gil, José Fernando Lukhovitskaya, Nina I. Flores, Betty Müller, Giovanna Chuquillanqui, Carlos Palomino Flores, Ladislao Monjane, Aderito Barker, Ian Kreuze, Jan Savenkov, Eugene I. Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination |
| title | Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination |
| title_full | Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination |
| title_fullStr | Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination |
| title_full_unstemmed | Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination |
| title_short | Molecular and pathobiological characterization of 61 Potato mop-top virus full-length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination |
| title_sort | molecular and pathobiological characterization of 61 potato mop top virus full length cdnas reveals great variability of the virus in the centre of potato domestication novel genotypes and evidence for recombination |
| topic | Diversifying selection Evolutionary divergence Genotype constellation Infectious full-length cDNA clone Multipartite virus Potato mop-top virus Reassortment Tecnología de modificación genética |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1185 https://doi.org/10.1111/mpp.12552 |
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