Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Universidad Nacional de Trujillo
2020
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| Acceso en línea: | https://repositorio.inia.gob.pe/handle/20.500.12955/1108 http://dx.doi.org/10.17268/sci.agropecu.2020.02.09 |
| _version_ | 1855490384009887744 |
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| author | Chumpitaz Bermejo, Astrid Caro Castro, Junior Cruz Hilacondo, Wilbert Eddy León Quispe, Jorge |
| author_browse | Caro Castro, Junior Chumpitaz Bermejo, Astrid Cruz Hilacondo, Wilbert Eddy León Quispe, Jorge |
| author_facet | Chumpitaz Bermejo, Astrid Caro Castro, Junior Cruz Hilacondo, Wilbert Eddy León Quispe, Jorge |
| author_sort | Chumpitaz Bermejo, Astrid |
| collection | Repositorio INIA |
| description | Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight". |
| format | Artículo |
| id | INIA1108 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Universidad Nacional de Trujillo |
| publisherStr | Universidad Nacional de Trujillo |
| record_format | dspace |
| spelling | INIA11082022-11-21T20:37:39Z Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary Chumpitaz Bermejo, Astrid Caro Castro, Junior Cruz Hilacondo, Wilbert Eddy León Quispe, Jorge Actinobacteria Phytophthora infestans Native potato Biocontrol of pests Antagonism Biotecnología agrícola Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight". 1.- Introduction. 2.- Materials and methods. 3.- Results and discussion. 4.- Conclusiones. References. 2020-06-12T06:05:24Z 2020-06-12T06:05:24Z 2020-05-31 info:eu-repo/semantics/article Chumpitaz, A.; Caro, J.; Cruz, W.; León, J. 2020. Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary. Scientia Agropecuaria 11(2): 223-231. https://repositorio.inia.gob.pe/handle/20.500.12955/1108 Scientia Agropecuaria http://dx.doi.org/10.17268/sci.agropecu.2020.02.09 eng Scientia Agropecuaria 11(2): 223 – 231 (2020) http://dx.doi.org/10.17268/sci.agropecu.2020.02.09 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/us/ application/pdf application/pdf Perú Universidad Nacional de Trujillo Perú Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Actinobacteria Phytophthora infestans Native potato Biocontrol of pests Antagonism Biotecnología agrícola Chumpitaz Bermejo, Astrid Caro Castro, Junior Cruz Hilacondo, Wilbert Eddy León Quispe, Jorge Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
| title | Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
| title_full | Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
| title_fullStr | Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
| title_full_unstemmed | Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
| title_short | Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
| title_sort | rhizospheric actinomycetes from organic crops of native potato solanum tuberosum isolation phenotypic characterization molecular identification and impact on biocontrol of phytophthora infestans mont de bary |
| topic | Actinobacteria Phytophthora infestans Native potato Biocontrol of pests Antagonism Biotecnología agrícola |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1108 http://dx.doi.org/10.17268/sci.agropecu.2020.02.09 |
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