Rapid plant DNA and RNA extraction protocol using a bench drill
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and R...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
FUNPEC-RP
2020
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| Materias: | |
| Acceso en línea: | https://repositorio.inia.gob.pe/handle/20.500.12955/1076 https://doi.org/10.4238/gmr18394 |
| _version_ | 1855028414540414976 |
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| author | Ferreira, C.F Gutiérrez Reynoso, Dina Lida Kreuze, Jan Iskra Caruana, M.L Chabannes, M Barbosa, A.C.O Santos, T.A Silva, A.G.S Amorim, E.P De Oliveira, S.A.S Jesus, O.N |
| author_browse | Amorim, E.P Barbosa, A.C.O Chabannes, M De Oliveira, S.A.S Ferreira, C.F Gutiérrez Reynoso, Dina Lida Iskra Caruana, M.L Jesus, O.N Kreuze, Jan Santos, T.A Silva, A.G.S |
| author_facet | Ferreira, C.F Gutiérrez Reynoso, Dina Lida Kreuze, Jan Iskra Caruana, M.L Chabannes, M Barbosa, A.C.O Santos, T.A Silva, A.G.S Amorim, E.P De Oliveira, S.A.S Jesus, O.N |
| author_sort | Ferreira, C.F |
| collection | Repositorio INIA |
| description | Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
| format | info:eu-repo/semantics/article |
| id | INIA1076 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | FUNPEC-RP |
| publisherStr | FUNPEC-RP |
| record_format | dspace |
| spelling | INIA10762022-11-21T20:42:34Z Rapid plant DNA and RNA extraction protocol using a bench drill Ferreira, C.F Gutiérrez Reynoso, Dina Lida Kreuze, Jan Iskra Caruana, M.L Chabannes, M Barbosa, A.C.O Santos, T.A Silva, A.G.S Amorim, E.P De Oliveira, S.A.S Jesus, O.N Inexpensive Plant nucleic acids extraction RNA pellet viability Tecnología de modificación genética Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. INTRODUCTION. MATERIAL AND METHODS. RESULTS AND DISCUSSION. ACKNOWLEDGEMENT. CONFLICTS OF INTEREST. REFERENCES. 2020-05-05T21:05:46Z 2020-05-05T21:05:46Z 2019-08-14 info:eu-repo/semantics/article Ferreira, C.F., Gutierrez, D.L., Kreuze, J.F., Iskra-Caruana, M.L., Chabannes, M., Barbosa, A.C.O., Santos, T.A., Silva, A.G.S., Santos, R.M.F., Amorim, E.P., Oliveira, S.A.S. de, Jesus, O.N. Rapid plant DNA and RNA extraction protocol using a bench drill. Genetics and Molecular Research. 18(3): GMR18394. https://repositorio.inia.gob.pe/handle/20.500.12955/1076 Genetics and Molecular Research https://doi.org/10.4238/gmr18394 eng Genetics and Molecular Research. 18(3): GMR18394. GMR;18394 https://www.geneticsmr.com/articles/rapid-plant-dna-and-rna-extraction-protocol-using-bench-drill info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ application/pdf application/pdf Brasil FUNPEC-RP Brasil Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Inexpensive Plant nucleic acids extraction RNA pellet viability Tecnología de modificación genética Ferreira, C.F Gutiérrez Reynoso, Dina Lida Kreuze, Jan Iskra Caruana, M.L Chabannes, M Barbosa, A.C.O Santos, T.A Silva, A.G.S Amorim, E.P De Oliveira, S.A.S Jesus, O.N Rapid plant DNA and RNA extraction protocol using a bench drill |
| title | Rapid plant DNA and RNA extraction protocol using a bench drill |
| title_full | Rapid plant DNA and RNA extraction protocol using a bench drill |
| title_fullStr | Rapid plant DNA and RNA extraction protocol using a bench drill |
| title_full_unstemmed | Rapid plant DNA and RNA extraction protocol using a bench drill |
| title_short | Rapid plant DNA and RNA extraction protocol using a bench drill |
| title_sort | rapid plant dna and rna extraction protocol using a bench drill |
| topic | Inexpensive Plant nucleic acids extraction RNA pellet viability Tecnología de modificación genética |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1076 https://doi.org/10.4238/gmr18394 |
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