Transcriptional analyses of two soybean cultivars under salt stress
Soybean is an economically important plant, and its production is affected in soils with high salinity levels. It is important to understand the adaptive mechanisms through which plants overcome this kind of stress and to identify potential genes for improving abiotic stress tolerance. RNA-Seq data...
| Autores principales: | , , , , |
|---|---|
| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2020
|
| Materias: | |
| Acceso en línea: | https://repositorio.inia.gob.pe/handle/20.500.12955/1075 https://doi.org/10.1007/s11033-020-05398-3 |
| _version_ | 1855028468771717120 |
|---|---|
| author | Cadavid, Isabel Cristina Guzmán Escuredo, Frank Lino De Olivera Busatto, Luisa De Almeida, Rita M.C Margis, Rogerio |
| author_browse | Cadavid, Isabel Cristina De Almeida, Rita M.C De Olivera Busatto, Luisa Guzmán Escuredo, Frank Lino Margis, Rogerio |
| author_facet | Cadavid, Isabel Cristina Guzmán Escuredo, Frank Lino De Olivera Busatto, Luisa De Almeida, Rita M.C Margis, Rogerio |
| author_sort | Cadavid, Isabel Cristina |
| collection | Repositorio INIA |
| description | Soybean is an economically important plant, and its production is affected in soils with high salinity levels. It is important to understand the adaptive mechanisms through which plants overcome this kind of stress and to identify potential genes for improving abiotic stress tolerance. RNA-Seq data of two Glycine max cultivars, a drought-sensitive (C08) and a tolerant (Conquista), subjected to different periods of salt stress were analyzed. The transcript expression profile was obtained using a transcriptogram approach, comparing both cultivars and different times of treatment. After 4 h of salt stress, Conquista cultivar had 1400 differentially expressed genes, 647 induced and 753 repressed. Comparative expression revealed that 719 genes share the same pattern of induction or repression between both cultivars. Among them, 393 genes were up- and 326 down-regulated. Salt stress also modified the expression of 54 isoforms of miRNAs in Conquista, by the maturation of 39 different pre-miRNAs. The predicted targets for 12 of those mature miRNAs also have matches with 15 differentially expressed genes from our analyses. We found genes involved in important pathways related to stress adaptation. Genes from both ABA and BR signaling pathways were modulated, with possible crosstalk between them, and with a likely post-transcriptional regulation by miRNAs. Genes related to ethylene biosynthesis, DNA repair, and plastid translation process were those that could be regulated by miRNA. |
| format | info:eu-repo/semantics/article |
| id | INIA1075 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INIA10752023-12-21T13:53:18Z Transcriptional analyses of two soybean cultivars under salt stress Cadavid, Isabel Cristina Guzmán Escuredo, Frank Lino De Olivera Busatto, Luisa De Almeida, Rita M.C Margis, Rogerio Abiotic stress Salt Glycine max miRNA Transcript Transcriptogram Tecnología de modificación genética Soybean is an economically important plant, and its production is affected in soils with high salinity levels. It is important to understand the adaptive mechanisms through which plants overcome this kind of stress and to identify potential genes for improving abiotic stress tolerance. RNA-Seq data of two Glycine max cultivars, a drought-sensitive (C08) and a tolerant (Conquista), subjected to different periods of salt stress were analyzed. The transcript expression profile was obtained using a transcriptogram approach, comparing both cultivars and different times of treatment. After 4 h of salt stress, Conquista cultivar had 1400 differentially expressed genes, 647 induced and 753 repressed. Comparative expression revealed that 719 genes share the same pattern of induction or repression between both cultivars. Among them, 393 genes were up- and 326 down-regulated. Salt stress also modified the expression of 54 isoforms of miRNAs in Conquista, by the maturation of 39 different pre-miRNAs. The predicted targets for 12 of those mature miRNAs also have matches with 15 differentially expressed genes from our analyses. We found genes involved in important pathways related to stress adaptation. Genes from both ABA and BR signaling pathways were modulated, with possible crosstalk between them, and with a likely post-transcriptional regulation by miRNAs. Genes related to ethylene biosynthesis, DNA repair, and plastid translation process were those that could be regulated by miRNA. 2020-05-05T21:04:53Z 2020-05-05T21:04:53Z 2020-03-29 info:eu-repo/semantics/article Cadavid, I.C., Guzman, F., de Oliveira-Busatto, L. et al. Transcriptional analyses of two soybean cultivars under salt stress. Mol Biol Rep 47, 2871–2888 (2020). doi: 10.1007/s11033-020-05398-3 https://repositorio.inia.gob.pe/handle/20.500.12955/1075 Molecular Biology Reports https://doi.org/10.1007/s11033-020-05398-3 eng Molecular Biology Reports 47, 2871–2888 (2020). https://link.springer.com/article/10.1007/s11033-020-05398-3 info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ application/pdf Brasil Springer Nature Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Abiotic stress Salt Glycine max miRNA Transcript Transcriptogram Tecnología de modificación genética Cadavid, Isabel Cristina Guzmán Escuredo, Frank Lino De Olivera Busatto, Luisa De Almeida, Rita M.C Margis, Rogerio Transcriptional analyses of two soybean cultivars under salt stress |
| title | Transcriptional analyses of two soybean cultivars under salt stress |
| title_full | Transcriptional analyses of two soybean cultivars under salt stress |
| title_fullStr | Transcriptional analyses of two soybean cultivars under salt stress |
| title_full_unstemmed | Transcriptional analyses of two soybean cultivars under salt stress |
| title_short | Transcriptional analyses of two soybean cultivars under salt stress |
| title_sort | transcriptional analyses of two soybean cultivars under salt stress |
| topic | Abiotic stress Salt Glycine max miRNA Transcript Transcriptogram Tecnología de modificación genética |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1075 https://doi.org/10.1007/s11033-020-05398-3 |
| work_keys_str_mv | AT cadavidisabelcristina transcriptionalanalysesoftwosoybeancultivarsundersaltstress AT guzmanescuredofranklino transcriptionalanalysesoftwosoybeancultivarsundersaltstress AT deoliverabusattoluisa transcriptionalanalysesoftwosoybeancultivarsundersaltstress AT dealmeidaritamc transcriptionalanalysesoftwosoybeancultivarsundersaltstress AT margisrogerio transcriptionalanalysesoftwosoybeancultivarsundersaltstress |