The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission
The main biological threat to the western honeybee (Apis mellifera) is the parasitic mite Varroa destructor, largely because it vectors lethal epidemics of honeybee viruses that, in the absence of this mite, are relatively innocuous. The severe pathology is a direct consequence of excessive virus ti...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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Springer Nature
2020
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| Online Access: | https://repositorio.inia.gob.pe/handle/20.500.12955/1061 https://doi.org/10.1038/s41598-020-62673-w |
| _version_ | 1855028596578451456 |
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| author | Yañez, Orlando Chávez Galarza, Julio César Tellgren Roth, Christian Pinto, María Alice Neumann, Peter R. de Miranda, Joachim |
| author_browse | Chávez Galarza, Julio César Neumann, Peter Pinto, María Alice R. de Miranda, Joachim Tellgren Roth, Christian Yañez, Orlando |
| author_facet | Yañez, Orlando Chávez Galarza, Julio César Tellgren Roth, Christian Pinto, María Alice Neumann, Peter R. de Miranda, Joachim |
| author_sort | Yañez, Orlando |
| collection | Repositorio INIA |
| description | The main biological threat to the western honeybee (Apis mellifera) is the parasitic mite Varroa destructor, largely because it vectors lethal epidemics of honeybee viruses that, in the absence of this mite, are relatively innocuous. The severe pathology is a direct consequence of excessive virus titres caused by this novel transmission route. However, little is known about how the virus adapts genetically during transmission and whether this influences the pathology. Here, we show that upon injection into honeybee pupae, the deformed wing virus type-A (DWV-A) quasispecies undergoes a rapid, extensive expansion of its sequence space, followed by strong negative selection towards a uniform, common shape by the time the pupae have completed their development, with no difference between symptomatic and asymptomatic adults in either DWV titre or genetic composition. This suggests that the physiological and molecular environment during pupal development has a strong, conservative influence on shaping the DWV-A quasispecies in emerging adults. There was furthermore no evidence of any progressive adaptation of the DWV-A quasispecies to serial intra-abdominal injection, simulating mite transmission, despite the generation of ample variation immediately following each transmission, suggesting that the virus either had already adapted to transmission by injection, or was unaffected by it. |
| format | info:eu-repo/semantics/article |
| id | INIA1061 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INIA10612022-11-21T20:43:47Z The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission Yañez, Orlando Chávez Galarza, Julio César Tellgren Roth, Christian Pinto, María Alice Neumann, Peter R. de Miranda, Joachim Abeja Occidental (Apis Mellifera) Ácaro Virus Composición Genética Tecnología de modificación genética The main biological threat to the western honeybee (Apis mellifera) is the parasitic mite Varroa destructor, largely because it vectors lethal epidemics of honeybee viruses that, in the absence of this mite, are relatively innocuous. The severe pathology is a direct consequence of excessive virus titres caused by this novel transmission route. However, little is known about how the virus adapts genetically during transmission and whether this influences the pathology. Here, we show that upon injection into honeybee pupae, the deformed wing virus type-A (DWV-A) quasispecies undergoes a rapid, extensive expansion of its sequence space, followed by strong negative selection towards a uniform, common shape by the time the pupae have completed their development, with no difference between symptomatic and asymptomatic adults in either DWV titre or genetic composition. This suggests that the physiological and molecular environment during pupal development has a strong, conservative influence on shaping the DWV-A quasispecies in emerging adults. There was furthermore no evidence of any progressive adaptation of the DWV-A quasispecies to serial intra-abdominal injection, simulating mite transmission, despite the generation of ample variation immediately following each transmission, suggesting that the virus either had already adapted to transmission by injection, or was unaffected by it. 1.- Results. 2.- Discussion. 3.- Methods. 4.- Acknowledgements. 2020-05-05T20:39:15Z 2020-05-05T20:39:15Z 2020-04-06 info:eu-repo/semantics/article Yañez, O., Chávez-Galarza, J., Tellgren-Roth, C. et al. El estado de desarrollo de la abeja melífera (Apis mellifera) da forma a la composición genética de la especie deformada del virus del ala A durante la transmisión en serie. Sci Rep. 10, 5956 (2020). doi: 10.1038/s41598-020-62673-w https://repositorio.inia.gob.pe/handle/20.500.12955/1061 Scientific Reports - Nature https://doi.org/10.1038/s41598-020-62673-w eng Scientific Reports. 10, 5956 (2020) ;5956 (2020) https://doi.org/10.1038/s41598-020-62673-w info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ application/pdf application/pdf Suecia Springer Nature Reindo Unido Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Abeja Occidental (Apis Mellifera) Ácaro Virus Composición Genética Tecnología de modificación genética Yañez, Orlando Chávez Galarza, Julio César Tellgren Roth, Christian Pinto, María Alice Neumann, Peter R. de Miranda, Joachim The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission |
| title | The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission |
| title_full | The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission |
| title_fullStr | The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission |
| title_full_unstemmed | The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission |
| title_short | The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission |
| title_sort | honeybee apis mellifera developmental state shapes the genetic composition of the deformed wing virus a quasispecies during serial transmission |
| topic | Abeja Occidental (Apis Mellifera) Ácaro Virus Composición Genética Tecnología de modificación genética |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/1061 https://doi.org/10.1038/s41598-020-62673-w |
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