Ploidy analysis in water yam, Dioscorea alata L., germplasm
Poor reproductive development in yams (Dioscorea spp.) has often been attributed to the polyploid nature of the crop. In this study, flow cytometry was used to determine the ploidy level of 53 accessions of Dioscorea alata, mostly from West African countries, Chad and Puerto Rico. Nuclei were isolat...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2002
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/99954 |
| _version_ | 1855530742389407744 |
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| author | Egesi, Chiedozie N. Pillay, M. Asiedu, A. Egunjobi, J.K. |
| author_browse | Asiedu, A. Egesi, Chiedozie N. Egunjobi, J.K. Pillay, M. |
| author_facet | Egesi, Chiedozie N. Pillay, M. Asiedu, A. Egunjobi, J.K. |
| author_sort | Egesi, Chiedozie N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Poor reproductive development in yams (Dioscorea spp.) has often been attributed to the polyploid nature of the crop. In this study, flow cytometry was used to determine the ploidy level of 53 accessions of Dioscorea alata, mostly from West African countries, Chad and Puerto Rico. Nuclei were isolated from young leaf material and stained with DAPI (4,6-diamidino-2-phenylindole). The nuclear genome size (2C) was measured as an indicator of the ploidy level. Dioscorea rotundata genotypes with known ploidy levels were used as standards. The results showed that the majority of plants were hexaploid (84.9%) with a smaller percentage of tetraploids (15.1%). A higher number of male plants were hexaploid than tetraploids. This is at variance with earlier findings, which reported that hexaploid male plants are rare. Higher ploidy levels were not directly related to sparse or erratic flowering as previously reported as profuse flowering occurred in some male hexaploid accessions. These findings have important implications for yam breeding in relation to yam genetic resources. |
| format | Journal Article |
| id | CGSpace99954 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| record_format | dspace |
| spelling | CGSpace999542023-06-12T17:05:00Z Ploidy analysis in water yam, Dioscorea alata L., germplasm Egesi, Chiedozie N. Pillay, M. Asiedu, A. Egunjobi, J.K. cytogenetics dioscorea alata flow cytometry yams Poor reproductive development in yams (Dioscorea spp.) has often been attributed to the polyploid nature of the crop. In this study, flow cytometry was used to determine the ploidy level of 53 accessions of Dioscorea alata, mostly from West African countries, Chad and Puerto Rico. Nuclei were isolated from young leaf material and stained with DAPI (4,6-diamidino-2-phenylindole). The nuclear genome size (2C) was measured as an indicator of the ploidy level. Dioscorea rotundata genotypes with known ploidy levels were used as standards. The results showed that the majority of plants were hexaploid (84.9%) with a smaller percentage of tetraploids (15.1%). A higher number of male plants were hexaploid than tetraploids. This is at variance with earlier findings, which reported that hexaploid male plants are rare. Higher ploidy levels were not directly related to sparse or erratic flowering as previously reported as profuse flowering occurred in some male hexaploid accessions. These findings have important implications for yam breeding in relation to yam genetic resources. 2002 2019-03-03T05:54:16Z 2019-03-03T05:54:16Z Journal Article https://hdl.handle.net/10568/99954 en Limited Access Egesi, C.N., Pillay, M., Asiedu, A. & Egunjobi, J.K. (2002). Ploidy analysis in water yam, Dioscorea alata L. germplasm. Euphytica 128(2), 225–230. |
| spellingShingle | cytogenetics dioscorea alata flow cytometry yams Egesi, Chiedozie N. Pillay, M. Asiedu, A. Egunjobi, J.K. Ploidy analysis in water yam, Dioscorea alata L., germplasm |
| title | Ploidy analysis in water yam, Dioscorea alata L., germplasm |
| title_full | Ploidy analysis in water yam, Dioscorea alata L., germplasm |
| title_fullStr | Ploidy analysis in water yam, Dioscorea alata L., germplasm |
| title_full_unstemmed | Ploidy analysis in water yam, Dioscorea alata L., germplasm |
| title_short | Ploidy analysis in water yam, Dioscorea alata L., germplasm |
| title_sort | ploidy analysis in water yam dioscorea alata l germplasm |
| topic | cytogenetics dioscorea alata flow cytometry yams |
| url | https://hdl.handle.net/10568/99954 |
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