Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level
An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
International Society for Horticultural Science
2000
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/99904 |
| _version_ | 1855520573228056576 |
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| author | Bosque Pérez, N.A. May, G. Artzen, C. |
| author_browse | Artzen, C. Bosque Pérez, N.A. May, G. |
| author_facet | Bosque Pérez, N.A. May, G. Artzen, C. |
| author_sort | Bosque Pérez, N.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains. |
| format | Journal Article |
| id | CGSpace99904 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | International Society for Horticultural Science |
| publisherStr | International Society for Horticultural Science |
| record_format | dspace |
| spelling | CGSpace999042024-08-27T10:35:25Z Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level Bosque Pérez, N.A. May, G. Artzen, C. genomics bananas clones musa agrobacterium An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains. 2000-10 2019-03-03T05:53:58Z 2019-03-03T05:53:58Z Journal Article https://hdl.handle.net/10568/99904 en Limited Access International Society for Horticultural Science Bosque-Perez, N., May, G. & Artzen, C. (2000). Applicability of an Agribacterium-based system for the transformation of Musa species with diverse genomic constitution of ploidy level. Acta Horticulturae, 540, 193-210. |
| spellingShingle | genomics bananas clones musa agrobacterium Bosque Pérez, N.A. May, G. Artzen, C. Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level |
| title | Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level |
| title_full | Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level |
| title_fullStr | Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level |
| title_full_unstemmed | Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level |
| title_short | Applicability of an Agribacteriumbased system for the transformation of Musa species with diverse genomic constitution of ploidy level |
| title_sort | applicability of an agribacteriumbased system for the transformation of musa species with diverse genomic constitution of ploidy level |
| topic | genomics bananas clones musa agrobacterium |
| url | https://hdl.handle.net/10568/99904 |
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