Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis
The bacterial leaf nodule symbiosis is a close interaction between endophytes and their plant hosts, mainly within the coffee family. The interaction between Rubiaceae species and Burkholderia bacteria is unique due to its obligate nature, high specificity, and predominantly vertical transmission of...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Public Library of Science
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/99406 |
| _version_ | 1855522589676404736 |
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| author | Sinnesael, A. Eeckhout, S. Janssens, S.B. Smets, E. Panis, Bartholomeus Leroux, O. Verstraete, B. |
| author_browse | Eeckhout, S. Janssens, S.B. Leroux, O. Panis, Bartholomeus Sinnesael, A. Smets, E. Verstraete, B. |
| author_facet | Sinnesael, A. Eeckhout, S. Janssens, S.B. Smets, E. Panis, Bartholomeus Leroux, O. Verstraete, B. |
| author_sort | Sinnesael, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The bacterial leaf nodule symbiosis is a close interaction between endophytes and their plant hosts, mainly within the coffee family. The interaction between Rubiaceae species and Burkholderia bacteria is unique due to its obligate nature, high specificity, and predominantly vertical transmission of the endophytes to the next generation of host plants. This vertical transmission is intriguing since it is the basis for the uniqueness of the symbiosis. However, unequivocal evidence of the location of the endophytes in the seeds is lacking. The aim of this paper is therefore to demonstrate the presence of the host specific endophyte in the seeds of Psychotria punctata and confirm its precise location. In addition, the suggested location of the endophyte in other parts of the host plant is investigated.
To identify and locate the endophyte in Psychotria punctata, a two-level approach was adopted using both a molecular screening method and fluorescent in situ hybridisation microscopy.
The endophytes, molecularly identified as Candidatus Burkholderia kirkii, were detected in the leaves, vegetative and flower buds, anthers, gynoecium, embryos, and young twigs. In addition, they were in situ localised in leaves, flowers and shoot apical meristems, and, for the first time, in between the cotyledons of the embryos.
Both independent techniques detected the host specific endophyte in close proximity to the shoot apical meristem of the embryo, which confirms for the first time the exact location of the endophytes in the seeds. This study provides reliable proof that the endophytes are maintained throughout the growth and development of the host plant and are transmitted vertically to the offspring. |
| format | Journal Article |
| id | CGSpace99406 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace994062025-11-12T05:41:00Z Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis Sinnesael, A. Eeckhout, S. Janssens, S.B. Smets, E. Panis, Bartholomeus Leroux, O. Verstraete, B. rubiaceae endophytes coffea vertical transmission The bacterial leaf nodule symbiosis is a close interaction between endophytes and their plant hosts, mainly within the coffee family. The interaction between Rubiaceae species and Burkholderia bacteria is unique due to its obligate nature, high specificity, and predominantly vertical transmission of the endophytes to the next generation of host plants. This vertical transmission is intriguing since it is the basis for the uniqueness of the symbiosis. However, unequivocal evidence of the location of the endophytes in the seeds is lacking. The aim of this paper is therefore to demonstrate the presence of the host specific endophyte in the seeds of Psychotria punctata and confirm its precise location. In addition, the suggested location of the endophyte in other parts of the host plant is investigated. To identify and locate the endophyte in Psychotria punctata, a two-level approach was adopted using both a molecular screening method and fluorescent in situ hybridisation microscopy. The endophytes, molecularly identified as Candidatus Burkholderia kirkii, were detected in the leaves, vegetative and flower buds, anthers, gynoecium, embryos, and young twigs. In addition, they were in situ localised in leaves, flowers and shoot apical meristems, and, for the first time, in between the cotyledons of the embryos. Both independent techniques detected the host specific endophyte in close proximity to the shoot apical meristem of the embryo, which confirms for the first time the exact location of the endophytes in the seeds. This study provides reliable proof that the endophytes are maintained throughout the growth and development of the host plant and are transmitted vertically to the offspring. 2018 2019-02-12T13:33:12Z 2019-02-12T13:33:12Z Journal Article https://hdl.handle.net/10568/99406 en Open Access application/pdf Public Library of Science Sinnesael, A.; Eeckhout, S.; Janssens, S.B.; Smets, E.; Panis, B.; Leroux, O.; Verstraete, B. (2018) Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis. PLoS ONE 13(12): e0209091. ISSN: 1932-6203 |
| spellingShingle | rubiaceae endophytes coffea vertical transmission Sinnesael, A. Eeckhout, S. Janssens, S.B. Smets, E. Panis, Bartholomeus Leroux, O. Verstraete, B. Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis |
| title | Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis |
| title_full | Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis |
| title_fullStr | Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis |
| title_full_unstemmed | Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis |
| title_short | Detection of Burkholderia in the seeds of Psychotria punctata (Rubiaceae) – Microscopic evidence for vertical transmission in the leaf nodule symbiosis |
| title_sort | detection of burkholderia in the seeds of psychotria punctata rubiaceae microscopic evidence for vertical transmission in the leaf nodule symbiosis |
| topic | rubiaceae endophytes coffea vertical transmission |
| url | https://hdl.handle.net/10568/99406 |
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