Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas
Brucellosis caused by the bacteria of the genus Brucella is an important zoonosis and constitutes a serious public health hazard. Brucellosis is diagnosed mainly by the Rose Bengal plate test and indirect ELISA, both of which have poor specificity because false positive serological reactions occur w...
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2018
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/98912 |
| _version_ | 1855534826588733440 |
|---|---|
| author | Kalleshamurthy, T. Shekar, R. Niranjanamurthy, H.H. Natesan, K. Shome, B.R. Bambal, R.G. Sairiwal, L. Barbuddhe, S.B. Sahare, A. Kilari, S. Rahman, Habibur Shome, R. |
| author_browse | Bambal, R.G. Barbuddhe, S.B. Kalleshamurthy, T. Kilari, S. Natesan, K. Niranjanamurthy, H.H. Rahman, Habibur Sahare, A. Sairiwal, L. Shekar, R. Shome, B.R. Shome, R. |
| author_facet | Kalleshamurthy, T. Shekar, R. Niranjanamurthy, H.H. Natesan, K. Shome, B.R. Bambal, R.G. Sairiwal, L. Barbuddhe, S.B. Sahare, A. Kilari, S. Rahman, Habibur Shome, R. |
| author_sort | Kalleshamurthy, T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Brucellosis caused by the bacteria of the genus Brucella is an important zoonosis and constitutes a serious public health hazard. Brucellosis is diagnosed mainly by the Rose Bengal plate test and indirect ELISA, both of which have poor specificity because false positive serological reactions occur when screening animals that have been vaccinated with B. abortus S19. Fluorescence polarization assay (FPA) was evaluated for screening samples from cattle and buffalo calves with persistent antibody titers induced by vaccination. The standardized FPA exhibited relative sensitivity and specificity of 0.94 and 0.95, respectively, and the area under the curve, kappa and accuracy were 0.98, 0.87 and 0.95, respectively. Comparison of competitive ELISA and FPA revealed that, FPA is more specific than competitive ELISA. The high specificity, sensitivity and 95% accuracy of FPA indicate that, it is suitable for testing vaccinated animals because it can distinguish between infected from vaccinated animals. |
| format | Journal Article |
| id | CGSpace98912 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace989122024-05-01T08:18:50Z Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas Kalleshamurthy, T. Shekar, R. Niranjanamurthy, H.H. Natesan, K. Shome, B.R. Bambal, R.G. Sairiwal, L. Barbuddhe, S.B. Sahare, A. Kilari, S. Rahman, Habibur Shome, R. animal diseases cattle health vaccines zoonoses Brucellosis caused by the bacteria of the genus Brucella is an important zoonosis and constitutes a serious public health hazard. Brucellosis is diagnosed mainly by the Rose Bengal plate test and indirect ELISA, both of which have poor specificity because false positive serological reactions occur when screening animals that have been vaccinated with B. abortus S19. Fluorescence polarization assay (FPA) was evaluated for screening samples from cattle and buffalo calves with persistent antibody titers induced by vaccination. The standardized FPA exhibited relative sensitivity and specificity of 0.94 and 0.95, respectively, and the area under the curve, kappa and accuracy were 0.98, 0.87 and 0.95, respectively. Comparison of competitive ELISA and FPA revealed that, FPA is more specific than competitive ELISA. The high specificity, sensitivity and 95% accuracy of FPA indicate that, it is suitable for testing vaccinated animals because it can distinguish between infected from vaccinated animals. 2018-11 2019-01-02T10:52:22Z 2019-01-02T10:52:22Z Journal Article https://hdl.handle.net/10568/98912 en Open Access Wiley Kalleshamurthy, T., Shekar, R., Niranjanamurthy, H.H., Natesan, K., Shome, B.R., Bambal, R.G., Sairiwal, L., Barbuddhe, S.B., Sahare, A., Kilari, S., Rahman, H. and Shome, R. 2018. Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas. Microbiology and Immunology 62(11):694-701. |
| spellingShingle | animal diseases cattle health vaccines zoonoses Kalleshamurthy, T. Shekar, R. Niranjanamurthy, H.H. Natesan, K. Shome, B.R. Bambal, R.G. Sairiwal, L. Barbuddhe, S.B. Sahare, A. Kilari, S. Rahman, Habibur Shome, R. Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas |
| title | Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas |
| title_full | Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas |
| title_fullStr | Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas |
| title_full_unstemmed | Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas |
| title_short | Assessment of fluorescence polarization assay: A candid diagnostic tool in Brucella abortus strain 19 vaccinated areas |
| title_sort | assessment of fluorescence polarization assay a candid diagnostic tool in brucella abortus strain 19 vaccinated areas |
| topic | animal diseases cattle health vaccines zoonoses |
| url | https://hdl.handle.net/10568/98912 |
| work_keys_str_mv | AT kalleshamurthyt assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT shekarr assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT niranjanamurthyhh assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT natesank assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT shomebr assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT bambalrg assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT sairiwall assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT barbuddhesb assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT saharea assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT kilaris assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT rahmanhabibur assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas AT shomer assessmentoffluorescencepolarizationassayacandiddiagnostictoolinbrucellaabortusstrain19vaccinatedareas |