Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly)
Trialeurodes vaporariorum (Westwood, 1856) (Greenhouse whitefly) is an agricultural pest of global importance. It is associated with damage to plants during feeding and subsequent virus transmission. Yet, global phylogenetic relationships, population structure, and estimation of the rates of gene fl...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Cambridge University Press
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/98858 |
| _version_ | 1855540176419291136 |
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| author | Wainaina, J.M. Barro, P. de Kubatko, L. Kehoe, M.A. Harvey, Jagger J.W. Karanja, D. Boykin, L.M. |
| author_browse | Barro, P. de Boykin, L.M. Harvey, Jagger J.W. Karanja, D. Kehoe, M.A. Kubatko, L. Wainaina, J.M. |
| author_facet | Wainaina, J.M. Barro, P. de Kubatko, L. Kehoe, M.A. Harvey, Jagger J.W. Karanja, D. Boykin, L.M. |
| author_sort | Wainaina, J.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Trialeurodes vaporariorum (Westwood, 1856) (Greenhouse whitefly) is an agricultural pest of global importance. It is associated with damage to plants during feeding and subsequent virus transmission. Yet, global phylogenetic relationships, population structure, and estimation of the rates of gene flow within this whitefly species remain largely unexplored. In this study, we obtained and filtered 227 GenBank records of mitochondrial cytochrome c oxidase I (mtCOI) sequences of T. vaporariorum, across various global locations to obtain a final set of 217 GenBank records. We further amplified and sequenced a ~750 bp fragment of mtCOI from an additional 31 samples collected from Kenya in 2014. Based on a total of 248 mtCOI sequences, we identified 16 haplotypes, with extensive overlap across all countries. Population structure analysis did not suggest population differentiation. Phylogenetic analysis indicated the 2014 Kenyan collection of samples clustered with a single sequence from the Netherlands to form a well-supported clade (denoted clade 1a) nested within the total set of sequences (denoted clade 1). Pairwise distances between sequences show greater sequence divergence between clades than within clades. In addition, analysis using migrate-n gave evidence for recent gene flow between the two groups. Overall, we find that T. vaporariorum forms a single large group, with evidence of further diversification consisting primarily of Kenyan sequences and one sequence from the Netherlands forming a well-supported clade. |
| format | Journal Article |
| id | CGSpace98858 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace988582024-04-25T06:01:49Z Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly) Wainaina, J.M. Barro, P. de Kubatko, L. Kehoe, M.A. Harvey, Jagger J.W. Karanja, D. Boykin, L.M. genetics trialeurodes vaporariorum Trialeurodes vaporariorum (Westwood, 1856) (Greenhouse whitefly) is an agricultural pest of global importance. It is associated with damage to plants during feeding and subsequent virus transmission. Yet, global phylogenetic relationships, population structure, and estimation of the rates of gene flow within this whitefly species remain largely unexplored. In this study, we obtained and filtered 227 GenBank records of mitochondrial cytochrome c oxidase I (mtCOI) sequences of T. vaporariorum, across various global locations to obtain a final set of 217 GenBank records. We further amplified and sequenced a ~750 bp fragment of mtCOI from an additional 31 samples collected from Kenya in 2014. Based on a total of 248 mtCOI sequences, we identified 16 haplotypes, with extensive overlap across all countries. Population structure analysis did not suggest population differentiation. Phylogenetic analysis indicated the 2014 Kenyan collection of samples clustered with a single sequence from the Netherlands to form a well-supported clade (denoted clade 1a) nested within the total set of sequences (denoted clade 1). Pairwise distances between sequences show greater sequence divergence between clades than within clades. In addition, analysis using migrate-n gave evidence for recent gene flow between the two groups. Overall, we find that T. vaporariorum forms a single large group, with evidence of further diversification consisting primarily of Kenyan sequences and one sequence from the Netherlands forming a well-supported clade. 2018-02 2018-12-20T13:32:35Z 2018-12-20T13:32:35Z Journal Article https://hdl.handle.net/10568/98858 en Open Access Cambridge University Press Wainaina, J.M., Barro, P. De, Kubatko, L., Kehoe, M.A., Harvey, J., Karanja, D. and Boykin, L.M. 2018. Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly). Bulletin of Entomological Research 108(1):5-13. |
| spellingShingle | genetics trialeurodes vaporariorum Wainaina, J.M. Barro, P. de Kubatko, L. Kehoe, M.A. Harvey, Jagger J.W. Karanja, D. Boykin, L.M. Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly) |
| title | Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly) |
| title_full | Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly) |
| title_fullStr | Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly) |
| title_full_unstemmed | Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly) |
| title_short | Global phylogenetic relationships, population structure and gene flow estimation of Trialeurodes vaporariorum (Greenhouse whitefly) |
| title_sort | global phylogenetic relationships population structure and gene flow estimation of trialeurodes vaporariorum greenhouse whitefly |
| topic | genetics trialeurodes vaporariorum |
| url | https://hdl.handle.net/10568/98858 |
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