Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis

DNA fingerprinting with synthetic simple repetitive oligonucleotides such as (CA)8 or (CAA)5 detected polymorphisms between various isolates of the ascomycete Mycosphaerella fijiensis, the causal agent of the black Sigatoka disease of Musa. These microsatellite motifs are present at multiple chromos...

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Main Authors: Muller, R., Pasberg-Gauhl, C., Gauhl, F., Ramser, J., Kahl, G.
Format: Journal Article
Language:Inglés
Published: Wiley 1997
Subjects:
Online Access:https://hdl.handle.net/10568/98645
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author Muller, R.
Pasberg-Gauhl, C.
Gauhl, F.
Ramser, J.
Kahl, G.
author_browse Gauhl, F.
Kahl, G.
Muller, R.
Pasberg-Gauhl, C.
Ramser, J.
author_facet Muller, R.
Pasberg-Gauhl, C.
Gauhl, F.
Ramser, J.
Kahl, G.
author_sort Muller, R.
collection Repository of Agricultural Research Outputs (CGSpace)
description DNA fingerprinting with synthetic simple repetitive oligonucleotides such as (CA)8 or (CAA)5 detected polymorphisms between various isolates of the ascomycete Mycosphaerella fijiensis, the causal agent of the black Sigatoka disease of Musa. These microsatellite motifs are present at multiple chromosomal locations and in high copy numbers in the Mycosphaerella genome, generating informative fingerprints with low background. Variability exists on a macro‐ as well as a microgeographical scale: it occurred within one lesion, between lesions of one plant, between plants, cultivars, and geographic locations. Mathematical analysis of the data produced dendrograms that demonstrated the presence of different genetically related groups of Mycosphaerella fijiensis in Nigeria.
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spelling CGSpace986452024-05-15T05:11:52Z Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis Muller, R. Pasberg-Gauhl, C. Gauhl, F. Ramser, J. Kahl, G. mycosphaerella fijiensis genetic variability DNA fingerprinting with synthetic simple repetitive oligonucleotides such as (CA)8 or (CAA)5 detected polymorphisms between various isolates of the ascomycete Mycosphaerella fijiensis, the causal agent of the black Sigatoka disease of Musa. These microsatellite motifs are present at multiple chromosomal locations and in high copy numbers in the Mycosphaerella genome, generating informative fingerprints with low background. Variability exists on a macro‐ as well as a microgeographical scale: it occurred within one lesion, between lesions of one plant, between plants, cultivars, and geographic locations. Mathematical analysis of the data produced dendrograms that demonstrated the presence of different genetically related groups of Mycosphaerella fijiensis in Nigeria. 1997-01 2018-12-19T07:01:05Z 2018-12-19T07:01:05Z Journal Article https://hdl.handle.net/10568/98645 en Limited Access Wiley Müller, R., Pasberg‐Gauhl, C., Gauhl, F., Ramser, J. & Kahl, G. (1997). Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis. Journal of Phytopathology, 145(1), 25-30.
spellingShingle mycosphaerella fijiensis
genetic variability
Muller, R.
Pasberg-Gauhl, C.
Gauhl, F.
Ramser, J.
Kahl, G.
Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis
title Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis
title_full Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis
title_fullStr Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis
title_full_unstemmed Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis
title_short Oligonucleotide fingerprinting detects genetic variability at different levels in Nigerian Mycosphaerella fijiensis
title_sort oligonucleotide fingerprinting detects genetic variability at different levels in nigerian mycosphaerella fijiensis
topic mycosphaerella fijiensis
genetic variability
url https://hdl.handle.net/10568/98645
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