Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos
Factors responsible for successful rescue of immature embryos of cowpea [Vigna unguiculata (L.) Walp.] and V. vexillata (L.) and for in vitro embryo development were studied. A new basal medium for embryo development in vitro was formulated on the basis of the mineral composition of embryos. Sucrose...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
1997
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/98622 |
| _version_ | 1855522215370424320 |
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| author | Pellegrineschi, A. Fatokun, C.A. Thottappilly, G. Adepoju, A.A. |
| author_browse | Adepoju, A.A. Fatokun, C.A. Pellegrineschi, A. Thottappilly, G. |
| author_facet | Pellegrineschi, A. Fatokun, C.A. Thottappilly, G. Adepoju, A.A. |
| author_sort | Pellegrineschi, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Factors responsible for successful rescue of immature embryos of cowpea [Vigna unguiculata (L.) Walp.] and V. vexillata (L.) and for in vitro embryo development were studied. A new basal medium for embryo development in vitro was formulated on the basis of the mineral composition of embryos. Sucrose, fructose and glucose were compared as carbohydrate sources. The highest frequency of embryos developing into plants was obtained with sucrose. Adding casein hydrolysate to the medium increased plant recovery by 30%. Among the plant growth factors used, cytokinins, zeatin, 6-benzylaminopurine and kinetin were the most effective in promoting embryo maturation and development. A method that can routinely ensure high plant recovery from cultured immature cowpea embryos is proposed. |
| format | Journal Article |
| id | CGSpace98622 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace986222024-08-27T10:35:37Z Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos Pellegrineschi, A. Fatokun, C.A. Thottappilly, G. Adepoju, A.A. vigna unguiculata cowpeas mineral salts Factors responsible for successful rescue of immature embryos of cowpea [Vigna unguiculata (L.) Walp.] and V. vexillata (L.) and for in vitro embryo development were studied. A new basal medium for embryo development in vitro was formulated on the basis of the mineral composition of embryos. Sucrose, fructose and glucose were compared as carbohydrate sources. The highest frequency of embryos developing into plants was obtained with sucrose. Adding casein hydrolysate to the medium increased plant recovery by 30%. Among the plant growth factors used, cytokinins, zeatin, 6-benzylaminopurine and kinetin were the most effective in promoting embryo maturation and development. A method that can routinely ensure high plant recovery from cultured immature cowpea embryos is proposed. 1997-12-01 2018-12-19T07:00:45Z 2018-12-19T07:00:45Z Journal Article https://hdl.handle.net/10568/98622 en Limited Access Springer Pellegrineschi, A., Fatokun, C.A., Thottappilly, G. & Adepoju, A.A. (1997). Cowpea embryo rescue. 1. Influence of culture media composition on plant recovery from isolated immature embryos. Plant Cell Reports, 17(2), 133-138. |
| spellingShingle | vigna unguiculata cowpeas mineral salts Pellegrineschi, A. Fatokun, C.A. Thottappilly, G. Adepoju, A.A. Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos |
| title | Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos |
| title_full | Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos |
| title_fullStr | Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos |
| title_full_unstemmed | Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos |
| title_short | Cowpea embryo rescue: influence of culture media composition on plant recovery from isolated immature embryos |
| title_sort | cowpea embryo rescue influence of culture media composition on plant recovery from isolated immature embryos |
| topic | vigna unguiculata cowpeas mineral salts |
| url | https://hdl.handle.net/10568/98622 |
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