Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages
In addition to assessing rate and extent of gas production from fermenting forages in vitro with rumen micro-organisms, gas production methods (e.g. Theodorou et at, 1994) may be used also to study the degradation kinetics of forage dry matter and its fractions. As the substrate dry matter can be lo...
| Autores principales: | , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
1998
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/98590 |
| _version_ | 1855524109090291712 |
|---|---|
| author | Dhanoa, M.S. Tanner, J.C. Owen, E. Theodorou, M.K. Winugroho, H.M. |
| author_browse | Dhanoa, M.S. Owen, E. Tanner, J.C. Theodorou, M.K. Winugroho, H.M. |
| author_facet | Dhanoa, M.S. Tanner, J.C. Owen, E. Theodorou, M.K. Winugroho, H.M. |
| author_sort | Dhanoa, M.S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In addition to assessing rate and extent of gas production from fermenting forages in vitro with rumen micro-organisms, gas production methods (e.g. Theodorou et at, 1994) may be used also to study the degradation kinetics of forage dry matter and its fractions. As the substrate dry matter can be lost only through fermentation or solution, this removes the error inherent in the polyester bag method caused by fine particle losses from bags being deemed part of the ‘soluble’ fraction. The pressure transducer technique (PTT) of Theodorou et al. (1994) was used to measure gas production from nine tropical forage samples (Table 1). Nine bottles were prepared from each of the forage samples. Two bottles of each forage were harvested after 8, 24 and 48 h of incubation and a further three bottles at 72 h, to determine dry matter (DM) and organic matter (OM) losses. The same nine forages were assessed using the polyester bag method (Mehrez and Ørskov, 1977) to obtain DM and OM disappearance after rumen incubations of 4, 8, 24, 48 and 72 h. The soluble fraction was determined by hand washing. The reproducibility of measured losses, at given times, was examined using concordance (rc ) correlation (Lin, 1989) and mean square prediction error (MSPE, Bibby and Toutenberg, 1977). Also the simple exponential model was used to estimate the fractional rate of DM degradation (kd ) and asymptote A (%) for each forage and the values obtained using PTT (kd,ptt ) and in situ (kd,bag) compared using rc and MSPE. |
| format | Journal Article |
| id | CGSpace98590 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace985902025-03-04T13:57:42Z Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages Dhanoa, M.S. Tanner, J.C. Owen, E. Theodorou, M.K. Winugroho, H.M. animal feeding forage mixed farming In addition to assessing rate and extent of gas production from fermenting forages in vitro with rumen micro-organisms, gas production methods (e.g. Theodorou et at, 1994) may be used also to study the degradation kinetics of forage dry matter and its fractions. As the substrate dry matter can be lost only through fermentation or solution, this removes the error inherent in the polyester bag method caused by fine particle losses from bags being deemed part of the ‘soluble’ fraction. The pressure transducer technique (PTT) of Theodorou et al. (1994) was used to measure gas production from nine tropical forage samples (Table 1). Nine bottles were prepared from each of the forage samples. Two bottles of each forage were harvested after 8, 24 and 48 h of incubation and a further three bottles at 72 h, to determine dry matter (DM) and organic matter (OM) losses. The same nine forages were assessed using the polyester bag method (Mehrez and Ørskov, 1977) to obtain DM and OM disappearance after rumen incubations of 4, 8, 24, 48 and 72 h. The soluble fraction was determined by hand washing. The reproducibility of measured losses, at given times, was examined using concordance (rc ) correlation (Lin, 1989) and mean square prediction error (MSPE, Bibby and Toutenberg, 1977). Also the simple exponential model was used to estimate the fractional rate of DM degradation (kd ) and asymptote A (%) for each forage and the values obtained using PTT (kd,ptt ) and in situ (kd,bag) compared using rc and MSPE. 1998 2018-12-14T17:05:15Z 2018-12-14T17:05:15Z Journal Article https://hdl.handle.net/10568/98590 en Limited Access Cambridge University Press Dhanoa, M.S., Tanner, J.C., Owen, E., Theodorou, M.K. and Winugroho, H.M. 1998. Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages. IN: In vitro techniques for measuring nutrient supply to ruminants. BSAP Occasional Publication 22: 249-251. |
| spellingShingle | animal feeding forage mixed farming Dhanoa, M.S. Tanner, J.C. Owen, E. Theodorou, M.K. Winugroho, H.M. Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages |
| title | Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages |
| title_full | Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages |
| title_fullStr | Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages |
| title_full_unstemmed | Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages |
| title_short | Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages |
| title_sort | use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages |
| topic | animal feeding forage mixed farming |
| url | https://hdl.handle.net/10568/98590 |
| work_keys_str_mv | AT dhanoams useofaninvitrogasproductionmethodtostudykineticsofrumendrymatterdegradationofforages AT tannerjc useofaninvitrogasproductionmethodtostudykineticsofrumendrymatterdegradationofforages AT owene useofaninvitrogasproductionmethodtostudykineticsofrumendrymatterdegradationofforages AT theodoroumk useofaninvitrogasproductionmethodtostudykineticsofrumendrymatterdegradationofforages AT winugrohohm useofaninvitrogasproductionmethodtostudykineticsofrumendrymatterdegradationofforages |