A method for generating virus-free cassava plants to combat viral disease epidemics in Africa
Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture, c...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/98432 |
| _version_ | 1855536213210955776 |
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| author | Maruthi, M.N. Whitfield, E.C. Otti, G. Tumwegamire, Silver Kanju, E. Legg, James P. Mkamilo, G. Kawuki, R.S. Benesi, I.R.M. Zacarias, A. Munga, T. Mwatuni, F. Mbugua, E. |
| author_browse | Benesi, I.R.M. Kanju, E. Kawuki, R.S. Legg, James P. Maruthi, M.N. Mbugua, E. Mkamilo, G. Munga, T. Mwatuni, F. Otti, G. Tumwegamire, Silver Whitfield, E.C. Zacarias, A. |
| author_facet | Maruthi, M.N. Whitfield, E.C. Otti, G. Tumwegamire, Silver Kanju, E. Legg, James P. Mkamilo, G. Kawuki, R.S. Benesi, I.R.M. Zacarias, A. Munga, T. Mwatuni, F. Mbugua, E. |
| author_sort | Maruthi, M.N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture, chemotherapy and thermotherapy. In the first cycle of our virus-indexing procedure, we successfully cleaned 27 of the 31 varieties (87%), and after an additional three cleaning cycles, all plants were virus-free. Virus-free tissue-cultured plants were shipped back to Africa for distribution to farmers. This first cross-boundary effort provides important lessons for mitigating the two-major cassava viral diseases. |
| format | Journal Article |
| id | CGSpace98432 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace984322025-11-11T10:42:34Z A method for generating virus-free cassava plants to combat viral disease epidemics in Africa Maruthi, M.N. Whitfield, E.C. Otti, G. Tumwegamire, Silver Kanju, E. Legg, James P. Mkamilo, G. Kawuki, R.S. Benesi, I.R.M. Zacarias, A. Munga, T. Mwatuni, F. Mbugua, E. cassava manihot esculenta cassava mosaic virus virus indexing east africa southern africa Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture, chemotherapy and thermotherapy. In the first cycle of our virus-indexing procedure, we successfully cleaned 27 of the 31 varieties (87%), and after an additional three cleaning cycles, all plants were virus-free. Virus-free tissue-cultured plants were shipped back to Africa for distribution to farmers. This first cross-boundary effort provides important lessons for mitigating the two-major cassava viral diseases. 2019-01 2018-12-04T14:25:08Z 2018-12-04T14:25:08Z Journal Article https://hdl.handle.net/10568/98432 en Open Access application/pdf Elsevier Maruthi, M.N., Whitfield, E.C., Otti, G., Tumwegamire, S., Kanju, E., Legg, J.P., ... & Mbugua, E. (2019). A method for generating virus-free cassava plants to combat viral disease epidemics in Africa. Physiological and Molecular Plant Pathology. |
| spellingShingle | cassava manihot esculenta cassava mosaic virus virus indexing east africa southern africa Maruthi, M.N. Whitfield, E.C. Otti, G. Tumwegamire, Silver Kanju, E. Legg, James P. Mkamilo, G. Kawuki, R.S. Benesi, I.R.M. Zacarias, A. Munga, T. Mwatuni, F. Mbugua, E. A method for generating virus-free cassava plants to combat viral disease epidemics in Africa |
| title | A method for generating virus-free cassava plants to combat viral disease epidemics in Africa |
| title_full | A method for generating virus-free cassava plants to combat viral disease epidemics in Africa |
| title_fullStr | A method for generating virus-free cassava plants to combat viral disease epidemics in Africa |
| title_full_unstemmed | A method for generating virus-free cassava plants to combat viral disease epidemics in Africa |
| title_short | A method for generating virus-free cassava plants to combat viral disease epidemics in Africa |
| title_sort | method for generating virus free cassava plants to combat viral disease epidemics in africa |
| topic | cassava manihot esculenta cassava mosaic virus virus indexing east africa southern africa |
| url | https://hdl.handle.net/10568/98432 |
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