An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)
We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/98372 |
| _version_ | 1855541292745883648 |
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| author | Behnam, Babak Bohórquez Cháux, Adriana Castañeda Méndez, Oscar F. Tsuji, Hiroyuki Ishitani, Manabu Becerra López Lavelle, Luis Augusto |
| author_browse | Becerra López Lavelle, Luis Augusto Behnam, Babak Bohórquez Cháux, Adriana Castañeda Méndez, Oscar F. Ishitani, Manabu Tsuji, Hiroyuki |
| author_facet | Behnam, Babak Bohórquez Cháux, Adriana Castañeda Méndez, Oscar F. Tsuji, Hiroyuki Ishitani, Manabu Becerra López Lavelle, Luis Augusto |
| author_sort | Behnam, Babak |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (170–600 μg/g) using low‐cost chemicals and consumables and with minimal contamination from polysaccharides, polyphenols, proteins, and other secondary metabolites. In particular, A260:A280 ratios were >2.0 for RNA from various tissues, and all of the present RNA samples yielded ribosomal integrity number values of greater than six. The resulting high purity and quality of isolated RNAs will facilitate downstream applications (quantitative reverse transcriptase‐polymerase chain reaction or RNA sequencing) in cassava molecular breeding. |
| format | Journal Article |
| id | CGSpace98372 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace983722025-03-13T09:45:41Z An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz) Behnam, Babak Bohórquez Cháux, Adriana Castañeda Méndez, Oscar F. Tsuji, Hiroyuki Ishitani, Manabu Becerra López Lavelle, Luis Augusto manihot esculenta rna cassava plant breeding yield We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (170–600 μg/g) using low‐cost chemicals and consumables and with minimal contamination from polysaccharides, polyphenols, proteins, and other secondary metabolites. In particular, A260:A280 ratios were >2.0 for RNA from various tissues, and all of the present RNA samples yielded ribosomal integrity number values of greater than six. The resulting high purity and quality of isolated RNAs will facilitate downstream applications (quantitative reverse transcriptase‐polymerase chain reaction or RNA sequencing) in cassava molecular breeding. 2018-11-24 2018-11-28T19:07:24Z 2018-11-28T19:07:24Z Journal Article https://hdl.handle.net/10568/98372 en Open Access Wiley Behnam, Babak; Bohorquez-Chaux, Adriana; Castañeda-mendez, Oscar Fernando; Tsuji, Hiroyuki; Ishitani, Manabu & Becerra Lopez-Lavalle, Luis Augusto (2018). An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz), FEBS Open Bio. In press |
| spellingShingle | manihot esculenta rna cassava plant breeding yield Behnam, Babak Bohórquez Cháux, Adriana Castañeda Méndez, Oscar F. Tsuji, Hiroyuki Ishitani, Manabu Becerra López Lavelle, Luis Augusto An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz) |
| title | An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz) |
| title_full | An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz) |
| title_fullStr | An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz) |
| title_full_unstemmed | An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz) |
| title_short | An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz) |
| title_sort | optimized isolation protocol yields high quality rna from cassava tissues manihot esculenta crantz |
| topic | manihot esculenta rna cassava plant breeding yield |
| url | https://hdl.handle.net/10568/98372 |
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