Cover Image
QR Code

Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene

Cassava torrado-like virus (CsTLV, Family Secoviridae) is found in mixed virus infections associated with severe disease symptoms in leaves and roots of cassava (Manihot esculenta Crantz, Euphorbiaceae), which is an important staple food crop for Africa, Asia and Latin America. The virus has been re...

Full description

Main Author: Jiménez Polo, Jenyfer
Format: Conference Proceedings
Language:Inglés
Published: 2018
Subjects:
cassava
manihot esculenta
viruses
plant diseases
Online Access:https://hdl.handle.net/10568/98288
_version_ 1855541635400597504
author Jiménez Polo, Jenyfer
author_browse Jiménez Polo, Jenyfer
author_facet Jiménez Polo, Jenyfer
author_sort Jiménez Polo, Jenyfer
collection Repository of Agricultural Research Outputs (CGSpace)
description Cassava torrado-like virus (CsTLV, Family Secoviridae) is found in mixed virus infections associated with severe disease symptoms in leaves and roots of cassava (Manihot esculenta Crantz, Euphorbiaceae), which is an important staple food crop for Africa, Asia and Latin America. The virus has been reported in Colombia, Peru and Argentina, where it has a significant effect on the accumulation of total sugars in cassava storage roots. CsTLV encodes an atypical Maf/HAM1 domain not found in other torradovirus genomes, but is present in heterologous viruses of the family Potyviridae also infecting euphorbiaceous hosts. Most viruses encode RNA silencing suppressors (RSS) to counteract the RNA silencing-based plant defense system. However no RSS has been yet identified in torradoviruses. To advance the characterization of CsTLV, agroinfiltration in Nicotiana benthamiana leaves of different viral genes was carried out using GFP as a reporter. Like the Maf/HAM1 domain found in Cassava brown streak virus (CBSV; Genus: Ipomovirus), the one encoded by CsTLV did not show RSS activity. On the other hand, we found that agroinfiltration of RNA2-ORF1 had a significant effect in the establishment of systemic infection by Cassava virus X (CsVX; Genus: Potexvirus), a virus lacking one of the Triple Gene Block genes (TGB3), which is involved in potexvirus movement. Although we observed that single infection of CsTLV increased the expression of GUS in of GUS-transgenic cassava lines, none of the CsTLV genes in this study showed RSS activity.
format Conference Proceedings
id CGSpace98288
institution CGIAR Consortium
language Inglés
publishDate 2018
publishDateRange 2018
publishDateSort 2018
record_format dspace
spelling CGSpace982882025-03-13T09:45:45Z Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene Jiménez Polo, Jenyfer cassava manihot esculenta viruses plant diseases Cassava torrado-like virus (CsTLV, Family Secoviridae) is found in mixed virus infections associated with severe disease symptoms in leaves and roots of cassava (Manihot esculenta Crantz, Euphorbiaceae), which is an important staple food crop for Africa, Asia and Latin America. The virus has been reported in Colombia, Peru and Argentina, where it has a significant effect on the accumulation of total sugars in cassava storage roots. CsTLV encodes an atypical Maf/HAM1 domain not found in other torradovirus genomes, but is present in heterologous viruses of the family Potyviridae also infecting euphorbiaceous hosts. Most viruses encode RNA silencing suppressors (RSS) to counteract the RNA silencing-based plant defense system. However no RSS has been yet identified in torradoviruses. To advance the characterization of CsTLV, agroinfiltration in Nicotiana benthamiana leaves of different viral genes was carried out using GFP as a reporter. Like the Maf/HAM1 domain found in Cassava brown streak virus (CBSV; Genus: Ipomovirus), the one encoded by CsTLV did not show RSS activity. On the other hand, we found that agroinfiltration of RNA2-ORF1 had a significant effect in the establishment of systemic infection by Cassava virus X (CsVX; Genus: Potexvirus), a virus lacking one of the Triple Gene Block genes (TGB3), which is involved in potexvirus movement. Although we observed that single infection of CsTLV increased the expression of GUS in of GUS-transgenic cassava lines, none of the CsTLV genes in this study showed RSS activity. 2018-07 2018-11-22T18:40:46Z 2018-11-22T18:40:46Z Conference Proceedings https://hdl.handle.net/10568/98288 en Open Access Jiménez Polo, Jenyfer. (2018). Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene. In: International Congress of Plant Pathology (ICPP) 2018: Plant Health in a Global Economy July 29- August 3-2018. 1 p.
spellingShingle cassava
manihot esculenta
viruses
plant diseases
Jiménez Polo, Jenyfer
Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene
title Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene
title_full Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene
title_fullStr Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene
title_full_unstemmed Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene
title_short Identification of a torradovirus-encoded protein that complements the systemic movement of a potexvirus lacking the TGB3 gene
title_sort identification of a torradovirus encoded protein that complements the systemic movement of a potexvirus lacking the tgb3 gene
topic cassava
manihot esculenta
viruses
plant diseases
url https://hdl.handle.net/10568/98288
work_keys_str_mv AT jimenezpolojenyfer identificationofatorradovirusencodedproteinthatcomplementsthesystemicmovementofapotexviruslackingthetgb3gene

Similar Items