Molecular and cytogenetic study of east African highland banana
East African highland bananas (EAHBs) are staple food crop in Uganda, Tanzania, Burundi, and other countries in the African Great Lakes region. Even though several morphologically different types exist, all EAHBs are triploid and display minimal genetic variation. To provide more insights into the g...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/98284 |
| _version_ | 1855523732864368640 |
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| author | Nemecková, A. Christelová, P. Cížková, J. Nyine, M. Houwe, Ines van den Svacina, R. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hribova, E. |
| author_browse | Christelová, P. Cížková, J. Doležel, Jaroslav Houwe, Ines van den Hribova, E. Nemecková, A. Nyine, M. Svacina, R. Swennen, Rony L. Uwimana, Brigitte |
| author_facet | Nemecková, A. Christelová, P. Cížková, J. Nyine, M. Houwe, Ines van den Svacina, R. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hribova, E. |
| author_sort | Nemecková, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | East African highland bananas (EAHBs) are staple food crop in Uganda, Tanzania, Burundi, and other countries in the African Great Lakes region. Even though several morphologically different types exist, all EAHBs are triploid and display minimal genetic variation. To provide more insights into the genetic variation within EAHBs, genotyping using simple sequence repeat (SSR) markers, molecular analysis of ITS1-5.8S-ITS2 region of ribosomal DNA locus, and the analysis of chromosomal distribution of ribosomal DNA sequences were done. A total of 38 triploid EAHB accessions available in the Musa germplasm collection (International Transit Centre, Leuven, Belgium) were characterized. Six diploid accessions of Musa acuminata ssp. zebrina, ssp. banksii, and ssp. malaccensis representing putative parents of EAHBs were included in the study. Flow cytometric estimation of 2C nuclear DNA content revealed small differences (max ~6.5%) in genome size among the EAHB clones. While no differences in the number of 45S and 5S rDNA loci were found, genotyping using 19 SSR markers resulted in grouping the EAHB accessions into four clusters. The DNA sequence analysis of the internal transcribed spacer region indicated a relation of EAHB clones with M. acuminata and, surprisingly, also with M. schizocarpa. The results suggest that EAHB cultivars originated from a single hybrid clone with M. acuminata ssp. zebrina and ssp. banksii being its most probable parents. Howeve |
| format | Journal Article |
| id | CGSpace98284 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace982842025-11-12T05:41:35Z Molecular and cytogenetic study of east African highland banana Nemecková, A. Christelová, P. Cížková, J. Nyine, M. Houwe, Ines van den Svacina, R. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hribova, E. bananas musa dna fluorescence genotypes East African highland bananas (EAHBs) are staple food crop in Uganda, Tanzania, Burundi, and other countries in the African Great Lakes region. Even though several morphologically different types exist, all EAHBs are triploid and display minimal genetic variation. To provide more insights into the genetic variation within EAHBs, genotyping using simple sequence repeat (SSR) markers, molecular analysis of ITS1-5.8S-ITS2 region of ribosomal DNA locus, and the analysis of chromosomal distribution of ribosomal DNA sequences were done. A total of 38 triploid EAHB accessions available in the Musa germplasm collection (International Transit Centre, Leuven, Belgium) were characterized. Six diploid accessions of Musa acuminata ssp. zebrina, ssp. banksii, and ssp. malaccensis representing putative parents of EAHBs were included in the study. Flow cytometric estimation of 2C nuclear DNA content revealed small differences (max ~6.5%) in genome size among the EAHB clones. While no differences in the number of 45S and 5S rDNA loci were found, genotyping using 19 SSR markers resulted in grouping the EAHB accessions into four clusters. The DNA sequence analysis of the internal transcribed spacer region indicated a relation of EAHB clones with M. acuminata and, surprisingly, also with M. schizocarpa. The results suggest that EAHB cultivars originated from a single hybrid clone with M. acuminata ssp. zebrina and ssp. banksii being its most probable parents. Howeve 2018-10-04 2018-11-22T14:04:19Z 2018-11-22T14:04:19Z Journal Article https://hdl.handle.net/10568/98284 en Open Access application/pdf Frontiers Media Němečková, A., Christelová, P., Čížková, J., Nyine, M., van den Houwe, I. Svačina, R., ... & Hribova, E. (2018). Molecular and cytogenetic study of East African Highland Banana. Frontiers in Plant Science, 9, 1371-1383. |
| spellingShingle | bananas musa dna fluorescence genotypes Nemecková, A. Christelová, P. Cížková, J. Nyine, M. Houwe, Ines van den Svacina, R. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hribova, E. Molecular and cytogenetic study of east African highland banana |
| title | Molecular and cytogenetic study of east African highland banana |
| title_full | Molecular and cytogenetic study of east African highland banana |
| title_fullStr | Molecular and cytogenetic study of east African highland banana |
| title_full_unstemmed | Molecular and cytogenetic study of east African highland banana |
| title_short | Molecular and cytogenetic study of east African highland banana |
| title_sort | molecular and cytogenetic study of east african highland banana |
| topic | bananas musa dna fluorescence genotypes |
| url | https://hdl.handle.net/10568/98284 |
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