Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa
Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli and its fuscans variant, X. axonopodis pv. phaseoli var. fuscans is a widespread disease of dry beans in South Africa. Variation within pathogen populations has been reported and in order to breed for resistance it is import...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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African Crop Science Society
2011
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| Acceso en línea: | https://hdl.handle.net/10568/97070 |
| _version_ | 1855527723919736832 |
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| author | Fourie, D. Herselman, L. |
| author_browse | Fourie, D. Herselman, L. |
| author_facet | Fourie, D. Herselman, L. |
| author_sort | Fourie, D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli and its fuscans variant, X. axonopodis pv. phaseoli var. fuscans is a widespread disease of dry beans in South Africa. Variation within pathogen populations has been reported and in order to breed for resistance it is important to investigate whether variation exists within the local pathogen population. One hundred and forty three common bacterial blight isolates from 44 localities in four countries, were inoculated onto eight Phaseolus acutifolius lines that differentiate between pathogenic races. This differential set was expanded to include resistant genotypes, XAN 159, GN #1 Nebr. sel 27, Wilk 2, Wilk 6, Vax 4, Vax 5 and Vax 6 and cv. Teebus as susceptible check. Genetic variation within nine selected Xap and Xapf isolates and a non-pathogenic Xanthomonas isolate, was studied using RAPD and AFLP analysis. Genotypes XAN 159, Wilk 2, Wilk 6, Vax 4, Vax 5 and Vax 6 were resistant to all isolates, while GN #1 Nebr. sel 27 and cv. Teebus were susceptible. Isolates varied in aggressiveness on cv. Teebus; however, the pathogenic reaction on the set of differentials indicated that all, but one isolate, grouped in what has been reported as race 2. Thus, results based on reaction of the majority isolates, suggest the absence of different races. However, the distinct differential reaction recorded for a single isolate, may prove to represent another, as yet unrecorded, race of this pathogen. Both RAPD and AFLP analyses revealed high frequency of DNA polymorphism among isolates and could distinguish between Xap, Xapf and a non-pathogenic isolate. Differences between Xap and Xapf isolates demonstrate that these are two distinct groups of bacteria. |
| format | Journal Article |
| id | CGSpace97070 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | African Crop Science Society |
| publisherStr | African Crop Science Society |
| record_format | dspace |
| spelling | CGSpace970702025-03-13T09:44:23Z Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa Fourie, D. Herselman, L. xanthomonas axonopodis common bacterial blight phaseolus vulgaris Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli and its fuscans variant, X. axonopodis pv. phaseoli var. fuscans is a widespread disease of dry beans in South Africa. Variation within pathogen populations has been reported and in order to breed for resistance it is important to investigate whether variation exists within the local pathogen population. One hundred and forty three common bacterial blight isolates from 44 localities in four countries, were inoculated onto eight Phaseolus acutifolius lines that differentiate between pathogenic races. This differential set was expanded to include resistant genotypes, XAN 159, GN #1 Nebr. sel 27, Wilk 2, Wilk 6, Vax 4, Vax 5 and Vax 6 and cv. Teebus as susceptible check. Genetic variation within nine selected Xap and Xapf isolates and a non-pathogenic Xanthomonas isolate, was studied using RAPD and AFLP analysis. Genotypes XAN 159, Wilk 2, Wilk 6, Vax 4, Vax 5 and Vax 6 were resistant to all isolates, while GN #1 Nebr. sel 27 and cv. Teebus were susceptible. Isolates varied in aggressiveness on cv. Teebus; however, the pathogenic reaction on the set of differentials indicated that all, but one isolate, grouped in what has been reported as race 2. Thus, results based on reaction of the majority isolates, suggest the absence of different races. However, the distinct differential reaction recorded for a single isolate, may prove to represent another, as yet unrecorded, race of this pathogen. Both RAPD and AFLP analyses revealed high frequency of DNA polymorphism among isolates and could distinguish between Xap, Xapf and a non-pathogenic isolate. Differences between Xap and Xapf isolates demonstrate that these are two distinct groups of bacteria. 2011 2018-09-04T16:27:22Z 2018-09-04T16:27:22Z Journal Article https://hdl.handle.net/10568/97070 en Open Access African Crop Science Society Fourie, D.; Herselman, L. (2011). Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa. African Crop Science Journal, 19(4): 393-407 |
| spellingShingle | xanthomonas axonopodis common bacterial blight phaseolus vulgaris Fourie, D. Herselman, L. Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa |
| title | Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa |
| title_full | Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa |
| title_fullStr | Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa |
| title_full_unstemmed | Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa |
| title_short | Pathogenic and genetic variation in Xanthomonas axonopodis pv. Phaseoli and its fuscans variant in Southern Africa |
| title_sort | pathogenic and genetic variation in xanthomonas axonopodis pv phaseoli and its fuscans variant in southern africa |
| topic | xanthomonas axonopodis common bacterial blight phaseolus vulgaris |
| url | https://hdl.handle.net/10568/97070 |
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