Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers
Twenty four arbitrarily selected cassava (Manihot esculenta Crantz) landraces, supplied by the germplasm collections in Kibaha and Ukiriguru research stations of Tanzania, were analysed by two different molecular marker techniques. Genetic distances on the basis of RAPD (random amplified polymorphic...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
African Journals Online
2004
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/96410 |
| _version_ | 1855531019431575552 |
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| author | Herzberg, F. Mahungu, N.M. Mignouna, J. Kullaya, A. |
| author_browse | Herzberg, F. Kullaya, A. Mahungu, N.M. Mignouna, J. |
| author_facet | Herzberg, F. Mahungu, N.M. Mignouna, J. Kullaya, A. |
| author_sort | Herzberg, F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Twenty four arbitrarily selected cassava (Manihot esculenta Crantz) landraces, supplied by the germplasm collections in Kibaha and Ukiriguru research stations of Tanzania, were analysed by two different molecular marker techniques. Genetic distances on the basis of RAPD (random amplified polymorphic DNA) revealed separate clustering of almost all coast region-derived varieties. Inter-sequence-tagged repeat (ISTR) analysis, the second method, reproduced the geographical segregation, though the effect was less pronounced. Two genotypes were found to be very similar with either method, indicating a possible double accession. There is high overall genetic variability of the cassava germplasm. However, the variability is non- homogenous; rather it represents varieties of the same geographical origin grouped together. These findings are useful in the rationalisation of sampling and management of germplasm collections. |
| format | Journal Article |
| id | CGSpace96410 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| publisher | African Journals Online |
| publisherStr | African Journals Online |
| record_format | dspace |
| spelling | CGSpace964102025-11-11T10:35:46Z Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers Herzberg, F. Mahungu, N.M. Mignouna, J. Kullaya, A. dna genetics manihot esculenta Twenty four arbitrarily selected cassava (Manihot esculenta Crantz) landraces, supplied by the germplasm collections in Kibaha and Ukiriguru research stations of Tanzania, were analysed by two different molecular marker techniques. Genetic distances on the basis of RAPD (random amplified polymorphic DNA) revealed separate clustering of almost all coast region-derived varieties. Inter-sequence-tagged repeat (ISTR) analysis, the second method, reproduced the geographical segregation, though the effect was less pronounced. Two genotypes were found to be very similar with either method, indicating a possible double accession. There is high overall genetic variability of the cassava germplasm. However, the variability is non- homogenous; rather it represents varieties of the same geographical origin grouped together. These findings are useful in the rationalisation of sampling and management of germplasm collections. 2004 2018-08-09T06:40:39Z 2018-08-09T06:40:39Z Journal Article https://hdl.handle.net/10568/96410 en Open Access application/pdf African Journals Online Herzberg, F., Mahungu, N.M., Mignouna, J. & Kullaya, A. (2004). Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers. African Crop Science Journal, 12(3), 171-187. |
| spellingShingle | dna genetics manihot esculenta Herzberg, F. Mahungu, N.M. Mignouna, J. Kullaya, A. Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers |
| title | Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers |
| title_full | Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers |
| title_fullStr | Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers |
| title_full_unstemmed | Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers |
| title_short | Assessment of genetic diversity of local varieties of cassava in Tanzania using molecular markers |
| title_sort | assessment of genetic diversity of local varieties of cassava in tanzania using molecular markers |
| topic | dna genetics manihot esculenta |
| url | https://hdl.handle.net/10568/96410 |
| work_keys_str_mv | AT herzbergf assessmentofgeneticdiversityoflocalvarietiesofcassavaintanzaniausingmolecularmarkers AT mahungunm assessmentofgeneticdiversityoflocalvarietiesofcassavaintanzaniausingmolecularmarkers AT mignounaj assessmentofgeneticdiversityoflocalvarietiesofcassavaintanzaniausingmolecularmarkers AT kullayaa assessmentofgeneticdiversityoflocalvarietiesofcassavaintanzaniausingmolecularmarkers |