Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding
Yams (Dioscorea spp.) constitute a staple food crop for over 100 million people in the humid and subhumid tropics. They are polyploid and vegetatively propagated. The Guinea yams, Dioscorea rotundata and D. cayenensis, are the most important yams in West and Central Africa where they are indigenous,...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Academic Journals
2003
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/96375 |
| _version_ | 1855530090501242880 |
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| author | Mignouna, Hodeba D. Abang, Mathew M. Asiedu, Robert |
| author_browse | Abang, Mathew M. Asiedu, Robert Mignouna, Hodeba D. |
| author_facet | Mignouna, Hodeba D. Abang, Mathew M. Asiedu, Robert |
| author_sort | Mignouna, Hodeba D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Yams (Dioscorea spp.) constitute a staple food crop for over 100 million people in the humid and subhumid tropics. They are polyploid and vegetatively propagated. The Guinea yams, Dioscorea rotundata and D. cayenensis, are the most important yams in West and Central Africa where they are indigenous, while D. alata (referred to as water yam) is the most widely distributed species globally. The genetics of yams is least understood among the major staple food crops due to several biological constraints and research neglect. Research to unravel the apparent complexity of the yam genome will have far-reaching implications for genetic improvement of this important tuber crop. Some progress has been made in recent years in germplasm characterization and the development of molecular markers for genome analysis. A genetic linkage map based on amplified fragment length polymorphism (AFLP) markers has been constructed for Guinea and water yams. These linkage maps were used to scan the genome for quantitative trait loci (QTL) associated with genes conferring resistance to Yam Mosaic Virus (YMV) in D. rotundata and anthracnose (Colletotrichum gloeosporioides) in D. alata. In addition, candidate random amplified polymorphic DNA (RAPD) markers associated with major genes controlling resistance to YMV and anthracnose have been identified that could be used for selection and pyramiding of YMV and anthracnose resistance genes in yam improvement. Also, molecular markers such as RAPDs, AFLPs, and microsatellites or simple sequence repeats (SSRs) have been developed for yam genome analysis. An initial c-DNA library has been constructed in order to develop expressed sequence tags (ESTs) for gene discovery and as a source of additional molecular markers. This paper will review the advances made, discuss the implications for yam genetic improvement and germplasm conservation, and outline the direction for future research. |
| format | Journal Article |
| id | CGSpace96375 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace963752024-05-15T05:11:50Z Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding Mignouna, Hodeba D. Abang, Mathew M. Asiedu, Robert yams dioscorea rotundata crop improvement modern biotechnology molecular breeding Yams (Dioscorea spp.) constitute a staple food crop for over 100 million people in the humid and subhumid tropics. They are polyploid and vegetatively propagated. The Guinea yams, Dioscorea rotundata and D. cayenensis, are the most important yams in West and Central Africa where they are indigenous, while D. alata (referred to as water yam) is the most widely distributed species globally. The genetics of yams is least understood among the major staple food crops due to several biological constraints and research neglect. Research to unravel the apparent complexity of the yam genome will have far-reaching implications for genetic improvement of this important tuber crop. Some progress has been made in recent years in germplasm characterization and the development of molecular markers for genome analysis. A genetic linkage map based on amplified fragment length polymorphism (AFLP) markers has been constructed for Guinea and water yams. These linkage maps were used to scan the genome for quantitative trait loci (QTL) associated with genes conferring resistance to Yam Mosaic Virus (YMV) in D. rotundata and anthracnose (Colletotrichum gloeosporioides) in D. alata. In addition, candidate random amplified polymorphic DNA (RAPD) markers associated with major genes controlling resistance to YMV and anthracnose have been identified that could be used for selection and pyramiding of YMV and anthracnose resistance genes in yam improvement. Also, molecular markers such as RAPDs, AFLPs, and microsatellites or simple sequence repeats (SSRs) have been developed for yam genome analysis. An initial c-DNA library has been constructed in order to develop expressed sequence tags (ESTs) for gene discovery and as a source of additional molecular markers. This paper will review the advances made, discuss the implications for yam genetic improvement and germplasm conservation, and outline the direction for future research. 2003-12 2018-08-09T06:40:34Z 2018-08-09T06:40:34Z Journal Article https://hdl.handle.net/10568/96375 en Open Access Academic Journals Mignouna, H.D., Abang, M.M. & Asiedu, R. (2003). Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding. African Journal of Biotechnology, 2(12), 478-485. |
| spellingShingle | yams dioscorea rotundata crop improvement modern biotechnology molecular breeding Mignouna, Hodeba D. Abang, Mathew M. Asiedu, Robert Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding |
| title | Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding |
| title_full | Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding |
| title_fullStr | Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding |
| title_full_unstemmed | Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding |
| title_short | Harnessing modern biotechnology for tropical tuber crop improvement: yam (Dioscorea spp.) molecular breeding |
| title_sort | harnessing modern biotechnology for tropical tuber crop improvement yam dioscorea spp molecular breeding |
| topic | yams dioscorea rotundata crop improvement modern biotechnology molecular breeding |
| url | https://hdl.handle.net/10568/96375 |
| work_keys_str_mv | AT mignounahodebad harnessingmodernbiotechnologyfortropicaltubercropimprovementyamdioscoreasppmolecularbreeding AT abangmathewm harnessingmodernbiotechnologyfortropicaltubercropimprovementyamdioscoreasppmolecularbreeding AT asiedurobert harnessingmodernbiotechnologyfortropicaltubercropimprovementyamdioscoreasppmolecularbreeding |